RHIZOBIAL INOCULATION AND P FERTILIZATION RESPONSE IN COMMON BEAN (PHASEOLUS VULGARIS) UNDER GLASSHOUSE AND FIELD CONDITIONS

2007 ◽  
Vol 43 (1) ◽  
pp. 67-77 ◽  
Author(s):  
M. ZAMAN-ALLAH ◽  
B. SIFI ◽  
B. L'TAIEF ◽  
M. H. EL AOUNI ◽  
J. J. DREVON
Keyword(s):  
Nitrogen Fixation ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Symbiotic Nitrogen Fixation ◽  
Field Conditions ◽  
Nutrient Deficiencies ◽  
P Fertilization ◽  
Rhizobium Tropici ◽  
Symbiotic Efficiency ◽  
Rhizobial Inoculation

Osmotic constraints, nutrient deficiencies, especially phosphorus, and the lack of efficient strains of rhizobia in Mediterranean soils are major factors limiting symbiotic nitrogen fixation and yield in common bean (Phaseolus vulgaris). In order to improve yields, we investigated responses to rhizobial inoculation and P fertilization under glasshouse and field conditions with two bean cultivars, Coco blanc and BAT477. The nodulation test, using a hydroponic system in a glasshouse, revealed strong variability among 22 rhizobium strains of various origins, in their symbiotic efficiency, compared with Rhizobium tropici CIAT899. The introduced strains proved to be more efficient although some local rhizobia revealed high potential efficiencies. Glasshouse trials showed a significant effect of inoculation and P supply on nodulation, N content and shoot dry weight that varied with rhizobial strain. Field trials substantiated the glasshouse observations and emphasized the effects of cultivar-rhizobia interactions on symbiotic parameters and yields. It is concluded that inoculation with suitable rhizobia with supply of additional P is a technology that may improve symbiotic nitrogen fixation and yield in common bean in some soils under a Mediterranean climate.

Effect of phosphorus deficiency on acid phosphatase and phytase activities in common bean (Phaseolus vulgaris L.) under symbiotic nitrogen fixation

Symbiosis ◽  
2009 ◽  
Vol 47 (3) ◽  
pp. 141-149 ◽  
Author(s):  
Saber Kouas ◽  
Julien Louche ◽  
Ahmed Debez ◽  
Claude Plassard ◽  
Jean Jacques Drevon ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Acid Phosphatase ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Symbiotic Nitrogen Fixation ◽  
Phosphorus Deficiency ◽  
Phaseolus Vulgaris L

scholarly journals Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Evdoxia Efstathiadou ◽  
Georgia Ntatsi ◽  
Dimitrios Savvas ◽  
Anastasia P. Tampakaki
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Phylogenetic Affiliation ◽  
Rhizobial Inoculation ◽  
Rhizobial Species ◽  
The Common ◽  
First Time ◽  
Of Greece ◽  
Rhizobial Isolates

AbstractPhaseolus vulgaris (L.), commonly known as bean or common bean, is considered a promiscuous legume host since it forms nodules with diverse rhizobial species and symbiovars. Most of the common bean nodulating rhizobia are mainly affiliated to the genus Rhizobium, though strains belonging to Ensifer, Pararhizobium, Mesorhizobium, Bradyrhizobium, and Burkholderia have also been reported. This is the first report on the characterization of bean-nodulating rhizobia at the species and symbiovar level in Greece. The goals of this research were to isolate and characterize rhizobia nodulating local common bean genotypes grown in five different edaphoclimatic regions of Greece with no rhizobial inoculation history. The genetic diversity of the rhizobial isolates was assessed by BOX-PCR and the phylogenetic affiliation was assessed by multilocus sequence analysis (MLSA) of housekeeping and symbiosis-related genes. A total of fifty fast-growing rhizobial strains were isolated and representative isolates with distinct BOX-PCR fingerpriniting patterns were subjected to phylogenetic analysis. The strains were closely related to R. anhuiense, R. azibense, R. hidalgonense, R. sophoriradicis, and to a putative new genospecies which is provisionally named as Rhizobium sp. I. Most strains belonged to symbiovar phaseoli carrying the α-, γ-a and γ-b alleles of nodC gene, while some of them belonged to symbiovar gallicum. To the best of our knowledge, it is the first time that strains assigned to R. sophoriradicis and harbored the γ-b allele were found in European soils. All strains were able to re-nodulate their original host, indicating that they are true microsymbionts of common bean.

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