Utilization and interconversions of purine derivatives in the fission yeastSchizosaccharomyces pombe

SUMMARYIn the fission yeastSchizosaccharomyces pombe, growth responses of mutants strains in the de novo purine synthesis pathway, in the purine interconversion system, and of various double mutants, have been studied upon different purine-supplemented media. The results show that exogenous purine utilization as nucleotide source is based exclusively upon pyrophosphorylation of purine bases, and they make it possible to identify most of the enzymic steps acting, in this organism, upon a purine ring to give another purine ring.A scheme of the interconversion system inSchizosaccharomyces pombeis given.

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De Novo Biosynthesis of Vanillin in Fission Yeast (Schizosaccharomyces pombe) and Baker's Yeast (Saccharomyces cerevisiae)

Applied and Environmental Microbiology ◽

10.1128/aem.02681-08 ◽

2009 ◽

Vol 75 (9) ◽

pp. 2765-2774 ◽

Cited By ~ 212

Author(s):

Esben H. Hansen ◽

Birger Lindberg Møller ◽

Gertrud R. Kock ◽

Camilla M. Bünner ◽

Charlotte Kristensen ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Schizosaccharomyces Pombe ◽

Fission Yeast ◽

De Novo ◽

Global Market ◽

Baker’S Yeast ◽

Yeast Cells ◽

Vanillyl Alcohol ◽

Baker's Yeast ◽

Yeast Saccharomyces Cerevisiae

ABSTRACT Vanillin is one of the world's most important flavor compounds, with a global market of 180 million dollars. Natural vanillin is derived from the cured seed pods of the vanilla orchid (Vanilla planifolia), but most of the world's vanillin is synthesized from petrochemicals or wood pulp lignins. We have established a true de novo biosynthetic pathway for vanillin production from glucose in Schizosaccharomyces pombe, also known as fission yeast or African beer yeast, as well as in baker's yeast, Saccharomyces cerevisiae. Productivities were 65 and 45 mg/liter, after introduction of three and four heterologous genes, respectively. The engineered pathways involve incorporation of 3-dehydroshikimate dehydratase from the dung mold Podospora pauciseta, an aromatic carboxylic acid reductase (ACAR) from a bacterium of the Nocardia genus, and an O-methyltransferase from Homo sapiens. In S. cerevisiae, the ACAR enzyme required activation by phosphopantetheinylation, and this was achieved by coexpression of a Corynebacterium glutamicum phosphopantetheinyl transferase. Prevention of reduction of vanillin to vanillyl alcohol was achieved by knockout of the host alcohol dehydrogenase ADH6. In S. pombe, the biosynthesis was further improved by introduction of an Arabidopsis thaliana family 1 UDP-glycosyltransferase, converting vanillin into vanillin β-d-glucoside, which is not toxic to the yeast cells and thus may be accumulated in larger amounts. These de novo pathways represent the first examples of one-cell microbial generation of these valuable compounds from glucose. S. pombe yeast has not previously been metabolically engineered to produce any valuable, industrially scalable, white biotech commodity.

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Ubiquitination regulates cytoophidium assembly in Schizosaccharomyces pombe

10.1101/2021.12.06.470909 ◽

2021 ◽

Author(s):

Christos Andreadis ◽

Tianhao Li ◽

Ji-Long Liu

Keyword(s):

Schizosaccharomyces Pombe ◽

Fission Yeast ◽

De Novo ◽

Metabolic Enzyme ◽

Filamentous Structure ◽

Deubiquitinating Enzymes ◽

Evolutionarily Conserved ◽

Ctp Synthase

AbstractCTP synthase (CTPS), a metabolic enzyme responsible for the de novo synthesis of CTP, can form filamentous structures termed cytoophidia, which are evolutionarily conserved from bacteria to humans. Here we used Schizosaccharomyces pombe to study the cytoophidium assembly regulation by ubiquitination. We tested the CTP synthase’s capacity to be epigenetically modified by ubiquitin or be affected by the ubiquitination state of the cell, showed that CTPS is immunoprecipitated with ubiquitin, and that ubiquitination is important for the maintenance of the CTPS filamentous structure in fission yeast. We have identified proteins which are in complex with CTPS, including specific ubiquitination regulators which significantly affect CTPS filamentation, and mapped probable ubiquitination targets on CTPS. Furthermore, we discovered that a cohort of deubiquitinating enzymes is significant for the regulation of cytoophidium morphology. Our study provides a framework for the analysis of the effects that ubiquitination and deubiquitination have on the formation of CTPS filaments.

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Effect of adenine on the synthesis of purine derivatives in wheat seedlings

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1972.021 ◽

2015 ◽

Vol 41 (2) ◽

pp. 283-288 ◽

Cited By ~ 3

Author(s):

Hanna Rybacka

Keyword(s):

De Novo ◽

Purine Derivatives ◽

Wheat Seedlings ◽

Purine Synthesis

The effect of adenine and other purine derivatives on the "de novo" purine synthesis was investigated in young wheat schoots. Adenine, adenosine and AMP inhibited strongly this biosynthesis, whereas hypoxanthine had no effect. The incorporation of each of the used precursors; [l-14C] glycine, [14C] formate and [14C] carbonate was inhibited by adenine in the same extent.

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Inhibition of de novo purine synthesis by deazatetrahydrofolates

Zurich, Switzerland, September 3–8, 1989 ◽

10.1515/9783110889000-209 ◽

1990 ◽

pp. 1080-1086

Keyword(s):

De Novo ◽

Purine Synthesis

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A new folate antimetabolite, 5,10-dideaza-5,6,7,8-tetrahydrofolate is a potent inhibitor of de novo purine synthesis

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)31261-9 ◽

1989 ◽

Vol 264 (1) ◽

pp. 328-333 ◽

Cited By ~ 2

Author(s):

G P Beardsley ◽

B A Moroson ◽

E C Taylor ◽

R G Moran

Keyword(s):

De Novo ◽

Potent Inhibitor ◽

Purine Synthesis

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Upstream – News in Genomics

Comparative and Functional Genomics ◽

10.1002/cfg.172 ◽

2002 ◽

Vol 3 (3) ◽

pp. 221-225

Keyword(s):

Saccharomyces Cerevisiae ◽

Ovarian Cancer ◽

Oryza Sativa ◽

Schizosaccharomyces Pombe ◽

Fission Yeast ◽

Proteomic Analysis ◽

Large Scale ◽

Protein Complexes ◽

The Other ◽

Blood Samples

In recent months a bumper crop of genomes has been completed, including the fission yeast (Schizosaccharomyces pombe) and rice (Oryza sativa). Two large-scale studies ofSaccharomyces cerevisiaeprotein complexes provided a picture of the eukaryotic proteome as a network of complexes. Amongst the other stories of interest was a demonstration that proteomic analysis of blood samples can be used to detect ovarian cancer, perhaps even as early as stage I.

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