Male sterility in field beans (Vicia faba L.). IV. Use of cytoplasmic male sterility in the production of F1 hybrids, and their performance in trials

1966 ◽  
Vol 66 (3) ◽  
pp. 369-377 ◽  
Author(s):  
D. A. Bond ◽  
J. L. Fyfe ◽  
Gillian Toynbee-Clarke
Keyword(s):  
Male Sterility ◽  
Large Scale ◽  
Female Parent ◽  
F1 Hybrids ◽  
Scale Production ◽  
Male Sterile ◽  
Large Scale Production ◽  
High Level ◽  
Tolerable Level ◽  
Field Beans

1. A method of producing hybrid beans with the aid of a cytoplasmic male-sterile is described.2. Most of the requirements of large scale production of hybrid seed can be met: use of genetic markers showed that the male sterility allowed 100% cross fertilization; there was no evidence of a deficiency of insect pollinators near Cambridge; and fertility restoring lines are available.3. The importance of being able to maintain a high level of sterility in the female parent line is emphasized. Methods of improving the proportion of sterile plants in the sterile phase of an inbred are discussed. The tolerable level of fertility in progenies of sterile parents depends on the level of sterility necessary in the female parent of the final cross. Five inbreds are being handled with less than 2% of fertile plants in their sterile phase.

Male sterility in field beans (Vicia faba L.). I. A male-sterile bean inherited as a Mendelian recessive

1964 ◽  
Vol 63 (2) ◽  
pp. 229-234 ◽  
Author(s):  
D. A. Bond ◽  
Jean M. Drayner ◽  
J. L. Fyfe ◽  
Gillian Toynbee-Clarke
Keyword(s):  
Male Sterility ◽  
Vicia Faba ◽  
Large Scale ◽  
Marker Gene ◽  
Scale Production ◽  
Male Sterile ◽  
Large Scale Production ◽  
Vicia Faba L ◽  
Suitable Marker ◽  
Field Beans

1. A male-sterile winter bean, inherited as a simple Mendelian recessive, is described.2. The sterility gene was shown to be expressed in the cytoplasm of eleven inbred lines of English winter beans, two varieties of English spring beans, six varieties of foreign spring beans and three varieties of broad beans (Vicia faba major). It is concluded that these varieties are unlikely to provide a fertility-inducing cytoplasm which would allow the development of a non-restoring pollinator.3. Evidence of linkage between three chlorophyll-deficient mutants and the male sterility locus is given and circumstances are outlined by which a suitable marker gene could be employed to assist in large-scale production of hybrid seed.

scholarly journals Male Sterility and its Commercial Exploitation in Hybrid Seed Production of Vegetable Crops: A Review

2019 ◽  
Vol 40 (04) ◽  
Author(s):  
Pramod Sharma ◽  
Sunil A Nair ◽  
Payal Sharma
Keyword(s):  
Male Sterility ◽  
Seed Production ◽  
Large Scale ◽  
Pollen Grains ◽  
Hybrid Seed ◽  
Scale Production ◽  
Hybrid Seed Production ◽  
Vegetable Crops ◽  
Male Sterile ◽  
Large Scale Production

Male sterility is described as absence of functional pollen grains in hermaphrodite flowers facilitating large scale production of hybrid seeds in vegetable crops. It eases hybrid seed production at commercial level in crops like tomato, chilli, capsicum, carrot, onion, cabbage, cauliflower and cucurbits. Male sterility would reduce the cost of hybrid seed production by limiting the labour making it efficient and economical. Incorporation of biotechnological tools in conventional plant breeding techniques would aid the breeders in limiting the drawbacks surrounding exploitation of male sterility for development of new hybrids. The present review is an attempt to summarize and to know the commercial utilization of male sterile line in hybrid seed production of vegetables.

scholarly journals Development of male sterile broccoli lines with raphanus sativus cytoplasm and assessment of their value for breeding purposes

2013 ◽  
Vol 21 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Piotr Kamiński
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Raphanus Sativus ◽  
Morphological Characters ◽  
F1 Hybrids ◽  
Vegetable Crops ◽  
Male Sterile ◽  
Self Compatibility ◽  
High Level ◽  
Research Institute

ABSTRACT The aim of this study was to obtain new broccoli lines with cytoplasmic male sterility trait for the development of the modern F1 hybrids. CDT70 cauliflower line obtained in the Research Institute of Horticulture, formerly Research Institute of Vegetable Crops, Skierniewice, Poland, with the reliable cytoplasmic male sterility from Raphanus sativus (Ogu-INRA) was selected as a source of this trait. Three broccoli lines: BMi, BCr1 and BCr2 were used as donors of commercial characters in all cross combinations with sterile components. Selected fertile broccoli genotypes were characterized by good quality, uniformity and high level of self-compatibility. The breeding procedure included three consecutive back crosses of male sterile genotypes with fertile broccoli lines that lasted from 2008 to 2012. In each generation, self-compatibility level, the stability of the male sterility trait and ability for the generative propagation of back-crossed genotypes were tested in comparison with donor broccoli lines in the greenhouse. The agronomical and morphological characters of the back-crossed progeny were also evaluated in the field. As a result, three CMS broccoli lines of Bc3 generation with good quality and high seeding index, suitable for the breeding purposes, were obtained.

scholarly journals High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris

2019 ◽  
Vol 21 (1) ◽  
pp. 279
Author(s):  
Qinghua Zhou ◽  
Zhixin Su ◽  
Liangcheng Jiao ◽  
Yao Wang ◽  
Kaixin Yang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Yarrowia Lipolytica ◽  
Large Scale ◽  
Gene Dosage ◽  
Life Time ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Scale Production ◽  
Large Scale Production ◽  
High Level

As a promising biocatalyst, Yarrowia lipolytica lipase 2 (YlLip2) is limited in its industrial applications due to its low thermostability. In this study, a thermostable YlLip2 mutant was overexpressed in Pichia pastoris and its half-life time was over 30 min at 80 °C. To obtain a higher protein secretion level, the gene dosage of the mutated lip2 gene was optimized and the lipase activity was improved by about 89%. Then, the YlLip2 activity of the obtained strain further increased from 482 to 1465 U/mL via optimizing the shaking flask culture conditions. Subsequently, Hac1p and Vitreoscilla hemoglobin (VHb) were coexpressed with the YlLip2 mutant to reduce the endoplasmic reticulum stress and enhance the oxygen uptake efficiency in the recombinant strains, respectively. Furthermore, high-density fermentations were performed in a 3 L bioreactor and the production of the YlLip2 mutant reached 9080 U/mL. The results demonstrated that the expression level of the thermostable YlLip2 mutant was predominantly enhanced via the combination of these strategies in P. pastoris, which forms a consolidated basis for its large-scale production and future industrial applications.

Interspecific introgression of male sterility from tetraploid oilseed Brassica napus to diploid vegetable B. rapa through hybridisation and backcrossing

2013 ◽  
Vol 64 (7) ◽  
pp. 652 ◽  
Author(s):  
Zhengjie Wan ◽  
Yuanbao Tan ◽  
Minhui Shi ◽  
Yuejin Xu ◽  
Nader Aryamanesh ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Morphological Characteristics ◽  
Female Parent ◽  
F1 Hybrids ◽  
Recurrent Parent ◽  
Male Sterile ◽  
Interspecific Introgression ◽  
C Genome ◽  
Inbreeding Line

Interspecific F1 hybrids were obtained from a cross between a male sterile Brassica napus (2n = 4x = 38, AA (20) and CC (18) genomes) and an inbreeding line B. rapa (Purple Cai-Tai inbred line 9418, 2n = 2x = 20, AA (20) genome) to introgress male sterility from a tetraploid into a diploid through backcrossing. The morphological characteristics of F1 plants were more like the female parent B. napus and segregated considerably in BC1 when backcrossed to the recurrent parent Purple Cai-Tai. The progeny became stable and more similar to Purple Cai-Tai by BC4. Most C genome chromosomes were found to be eliminated, based on cytogenetic analysis. The majority of chromosomes were eliminated at very early backcross stages, with only 20–26 chromosomes in BC1 plants, and some chromosomes were eliminated gradually with increased backcross generations. The BC4 plants were generally stable with exactly 20 chromosomes. Analysis by AFLP indicated that 49.5–68.7% of the total bands eliminated from F1 to BC4 were female parent specific, and ~12% of B. napus bands were retained with increased backcrossing. The genetic materials controlling sterility from the female parent B. napus were introgressed successfully into the BC4 plants even though most B. napus chromosomes/genetic materials were eliminated during the backcross process.

scholarly journals Differential Resistance to Botryosphaeria ribis Among Cercis Taxa

Plant Disease ◽  
2002 ◽  
Vol 86 (8) ◽  
pp. 880-882 ◽  
Author(s):  
M. R. Pooler ◽  
K. A. Jacobs ◽  
M. Kramer
Keyword(s):  
Large Scale ◽  
Spring Bloom ◽  
Differential Resistance ◽  
Early Spring ◽  
Scale Production ◽  
Small Tree ◽  
Independent Variables ◽  
Large Scale Production ◽  
High Level ◽  
Unexplained Variation

The redbud (Cercis sp.) is a popular ornamental small tree or shrub, valued commercially for its early spring bloom and adaptability to diverse environmental conditions. Despite these characteristics, large-scale production of redbud has been limited, due in part to their susceptibility to a fungal canker caused by Botryosphaeria ribis. We screened 711 plants in 11 Cercis taxa for response to inoculation with B. ribis. The taxa native to North America, C. canadensis and C. occidentalis, were more susceptible than Asian species. A logistic regression of the number of symptomatic plants 10 weeks postinoculation with taxa and size (stem diameter) as independent variables explained 41% of the variation. Sixteen percent was attributable to taxon effects and 36% was attributable to taxon-independent size effects. Size and taxon effects were not completely orthogonal, and taxa with larger mean stem diameters generally had higher percentages of symptomless plants. A high level of unexplained variation (59%) was found, and is likely due to intraspecific variation among seed lots. Comparisons of 11 seed lots of C. canadensis revealed significantly different proportions of diseased plants ranging from 52 to 92% after 10 weeks, but all plants eventually became diseased.

scholarly journals CLONAL MICROPROPAGATION OF MALE-STERILE ZINNIA ELEGANS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1124F-1124
Author(s):  
R.B. Rogers ◽  
M.A.L. Smith ◽  
R. Cowen
Keyword(s):  
Large Scale ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Zinnia Elegans ◽  
Scale Production ◽  
Male Sterile ◽  
Clonal Micropropagation ◽  
Large Scale Production ◽  
High Humidity Environment

The only method for large scale production of pure hybrid seed in Zinnia elegans involves the use of male sterile individuals. The male sterile trait, however, is a three gene recessive which at best produces only 50% male sterile progeny from seed. Since no method of clonal propagation is available, seed-produced female lines require labor intensive field roguing to insure removal of all normal flowered individuals. Clonal micropropagation was investigated as a means of mass producing male steriles for use as female lines. Sterilization procedures were developed for seed and axillary bud explants. Shoot proliferation media containing various levels of BAP, 2ip, and kinetin were screened using in vitro germinated seedling explants of the inbred line `Orange Starlight'. Microshoots demonstrated a high rooting percentage after 2 weeks on basal medium without growth regulators. Plantlets were easily acclimated in 1 to 2 weeks in a high humidity environment. In vitro derived plants of identified male sterile plants were phenotypically evaluated as to their suitability for use in field production.

Male sterility in field beans (Vicia faba L.). II. Yield trials of F1 hybrid winter beans produced with the aid of male sterility

1964 ◽  
Vol 63 (2) ◽  
pp. 235-243 ◽  
Author(s):  
D. A. Bond ◽  
J. L. Fyfe ◽  
Gillian Toynbee-Clarke
Keyword(s):  
Male Sterility ◽  
Inbred Lines ◽  
F1 Hybrids ◽  
Male Sterile ◽  
Male Sterile Line ◽  
F1 Hybrid ◽  
Vicia Faba L ◽  
The Mean ◽  
Yield Trials ◽  
Field Beans

1. F1 hybrids between inbred lines of winter beans were obtained by roguing male-fertile plants from segregating progenies of a genetic male-sterile line which had been interplanted with a pollinator.2. In a series of trials the mean yields of many hybrids were significantly higher than those of open-pollinated varieties. Over the 4 years, 1959–62, with trials at three centres in each year, the mean advantages of all hybrids over the two control varieties was 6·4 cwt./acre over Gartons S.Q., 4·7 cwt./acre over Throws M.S.

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