Evaluation of polyethylene glycol test of measuring cell membrane stability as a drought tolerance test in wheat

1988 ◽  
Vol 110 (3) ◽  
pp. 429-433 ◽  
Author(s):  
G. S. Premachandra ◽  
T. Shimada
Keyword(s):  
Polyethylene Glycol ◽  
Cell Membrane ◽  
Drought Tolerance ◽  
Tolerance Test ◽  
Membrane Stability ◽  
Measuring Cell ◽  
Cell Membrane Stability ◽  
Drought Tolerant ◽  
Leaf Tissues ◽  
Wheat Lines

SummaryEffectiveness of the polyethylene glycol (PEG) test for measuring cell membrane stability (CMS) to select drought-tolerant genotypes in wheat was investigated. PEG test was compared with two other tests. Genetic variability of CMS was also investigated.Drought was induced artificially in pot-grown plants and in excised leaves, and percentage injury in leaf tissues by drought stress as measured by CMS was compared with that by PEG test. Percentage injury in leaf tissues of pot-grown plants was not correlated significantly with that by PEG test. However, percentage injury in excised leaves was well correlated with that in PEG test. The results suggest that the PEG test has merit in measuring drought tolerance in wheat. Frequency distribution of CMS in winter wheat lines was closely related to the characteristic pattern of quantitative inheritance and therefore CMS of wheat seems to be controlled by polygene action.

Drought tolerance assessment of African cowpea accessions based on stomatal behaviour and cell membrane stability

2008 ◽  
Vol 146 (6) ◽  
pp. 689-694 ◽  
Author(s):  
M. T. LABUSCHAGNE ◽  
R. VERHOEVEN ◽  
M. NKOUANESSI
Keyword(s):  
Cell Membrane ◽  
Drought Tolerance ◽  
Membrane Stability ◽  
Sub Saharan Africa ◽  
Cell Membrane Stability ◽  
Drought Tolerant ◽  
Stomatal Behaviour ◽  
Wide Range ◽  
Drought Conditions ◽  
Sub Saharan

SUMMARYIn Sub-Saharan Africa, cowpea is well known for its ability to survive under conditions of water stress and it plays an important role in regions where drought is the factor most limiting to crop yield. In the present study, the drought tolerance levels of 20 African cowpea accessions from three countries were evaluated. A number of the genotypes showed drought tolerance, the merits of stomatal behaviour and cell membrane stability to assess drought tolerance was demonstrated. Damage to the cell membranes caused by drought was less in tolerant accessions. Stomatal opening was also better regulated; the opening was smaller under drought conditions, thus reducing transpiration (T). The wide range of drought tolerance observed among the accessions suggests the possibility of breeding drought-tolerant cultivars in cowpea. Drought-tolerant accessions included Bafoussam 1, M.66, Bafoussam 3, Hluhluwa, Bafoussam 4, Balen, Makueni, Bafoussam 2 and Okhalweni and these could be recommended to breeders as valuable material for drought tolerance improvement in cowpea.

scholarly journals Evaluation of polyethylene glycol test for measurement of cell membrane stability in maize

1989 ◽  
Vol 35 (4) ◽  
pp. 565-573 ◽  
Author(s):  
Gnanasiri S. Premachandra ◽  
Hirohumi Saneoka ◽  
Shoitsu Ogata
Keyword(s):  
Polyethylene Glycol ◽  
Cell Membrane ◽  
Membrane Stability ◽  
Cell Membrane Stability

Cell membrane stability, an indicator of drought tolerance, as affected by applied nitrogen in soyabean

1990 ◽  
Vol 115 (1) ◽  
pp. 63-66 ◽  
Author(s):  
G. S. Premachandra ◽  
H. Saneoka ◽  
S. Ogata
Keyword(s):  
Cell Membrane ◽  
Osmotic Potential ◽  
Membrane Stability ◽  
Leaf Water ◽  
Nutrient Concentrations ◽  
Leaf Water Relations ◽  
N Application ◽  
Cell Membrane Stability ◽  
Application Rates ◽  
Leaf Tissues

SUMMARYFour soyabean cultivars were grown with two N application rates (50 and 300 kg N/ha) in the field at Hiroshima University, Japan, from June to August 1988. Cell membrane stability (CMS) by the polyethylene glycol (PEG) test, leaf water relations and nutrient concentrations in cell sap and leaf tissues were measured when the plants were 50 days old, in the uppermost fully expanded leaves.Cell membrane stability was higher at the higher N rate, the increase over the lower rate being greater in the cultivars Lee+ and Lee–than in Tamahomare and T201. Leaf water potential was not affected by the higher rate of N application. Osmotic adjustment, which was independent of water stress, was observed with the higher rate of N and it was higher in Lee + and Lee–than in Tamahomare and T201. It is suggested that osmotic potential in leaf tissues may influence CMS measured by the PEG test. Solute concentrations in cell sap and leaf tissues were higher at the higher N rate. Sugar and K were the major contributors to osmotic potential.

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