Genotypic and technological characterization of enterococci isolated from artisanal Fiore Sardo cheese

2004 ◽  
Vol 71 (4) ◽  
pp. 444-450 ◽  
Author(s):  
Sofia Cosentino ◽  
M Barbara Pisano ◽  
Arianna Corda ◽  
M Elisabetta Fadda ◽  
Carla Piras
Keyword(s):  
Wide Spectrum ◽  
Health Concern ◽  
Pcr Analysis ◽  
Gelatinase Activity ◽  
Rapd Pcr ◽  
Vancomycin Resistant ◽  
Low Incidence ◽  
Almost All ◽  
Hydrolyse Casein

A total of 118 enterococcal strains isolated from artisanal Fiore Sardo cheese were characterized technologically and genetically. The presence of potential virulence factors was also investigated. Strains were classified as Ec. faecium (84 strains), Ec. durans (24 strains) and Ec. faecalis (10 strains). RAPD-PCR analysis with two different primers (M13 and XD9) confirmed species identification and proved useful for the detection of interstrain variations, especially among Ec. faecium isolates. Most strains could hydrolyse casein and had weak acidifying activity in milk. None of the isolates produced lipolytic reactions. Gelatinase activity was observed in two strains of Ec. faecalis and one strain of Ec. durans. β-Haemolysis on horses' blood was never detected in any of the strains, independently of species. Most strains produced tyramine from tyrosine but none decarboxylated lysine, histidine or ornithine. Overall, a wide spectrum of resistance was observed. Almost all strains were resistant to the aminoglycosides, gentamycin, kanamycin, streptomycin, neomycin, and to the semisynthetic penicillin, oxacillin, but resistance to vancomycin was not widespread among our strains: only one Ec. faecium and one Ec. durans strain were found to be vancomycin resistant. Our results show a certain diversity in technological traits of the enterococcal strains isolated from artisanal Fiore Sardo, together with a low incidence of some potentially pathogenic traits of health concern.

scholarly journals Application of molecular methods for characterization of Botrytis fabae and Botrytis cinerea of faba bean

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 314-318
Author(s):  
N.M. Abou-Zeid ◽  
I.H. Dorriah ◽  
A.A. Marwa
Keyword(s):  
Botrytis Cinerea ◽  
Faba Bean ◽  
Morphological Characters ◽  
Molecular Methods ◽  
Pcr Analysis ◽  
Conidial Morphology ◽  
Rapd Pcr ◽  
Botrytis Fabae

Application of the RAPD methods allowed to clearly characterization of isolates of Botrytis fabae and isolates of B. cinerea. Results from RAPD-PCR analysis indicate different groups. Clusters were related with groups based on conidial morphology, morphological characters of the isolates of Botrytis spp.

scholarly journals Strain Improvement and Genetic Characterization of Indigenous Aspergillus Flavus for Amylolytic Potential

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400
Author(s):  
Sobiya Shafique ◽  
Rukhsana Bajwa ◽  
Shazia Shafique
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Flavus ◽  
Production Control ◽  
Strain Improvement ◽  
Chemical Mutagenesis ◽  
Pcr Analysis ◽  
Native Strain ◽  
Rapd Pcr ◽  
Mutant Strains

Aspergillus flavus FCBP–231, a filamentous fungus, was genetically modified for its ability to reveal extra cellular α-amylase activity. For strain improvement, the selected strains were subjected to UV irradiation (5-40 min exposure) and EMS treatment (50–300 μg mL−1) for hyper activity of an α-amylase enzyme. The mutants were quantitatively compared with the parental strain. UV and chemical mutagenesis brought about a dramatic enhancement in enzymatic activity. The mutant strains Af-UV-5.3 and Af-Ch-5.7 exhibited 79 and 110% more enzyme activity than the native strain A. flavus FCBP-231. This improvement in enzyme activity of the mutants suggests that they are suitable strains to be used in biotechnology. RAPD–PCR analysis revealed different patterns of amplicons of native as well as mutant derivatives, which suggested that the mutation imparted changes in the genetic make up of the mutants probably involved enzyme production control.

Characterization of a unique population of Fusarium oxysporum f.sp. cubense causing Fusarium wilt in Cavendish bananas at Carnarvon, Western Australia

1995 ◽  
Vol 46 (1) ◽  
pp. 167 ◽  
Author(s):  
KG Pegg ◽  
RG Shivas ◽  
NY Moore ◽  
S Bentley
Keyword(s):  
Fusarium Oxysporum ◽  
Vegetative Compatibility ◽  
Pcr Analysis ◽  
Banding Patterns ◽  
Vegetative Compatibility Groups ◽  
Rapd Pcr ◽  
Race 1 ◽  
Race 4 ◽  
Volatile Production

A unique population of Fusarium oxysporum f. sp. cubense affecting Cavendish cv. Williams banana plants was characterized using vegetative compatibility, volatile production, RAPD-PCR analysis, pectic enzyme production and pathogenicity. The isolates were more like race 1 isolates than race 4 isolates, although they were capable of attacking Cavendish clones. The Carnarvon isolates did not belong to any of the vegetative compatibility groups (VCGs) known to occur in Australia or overseas; they belonged in the 'inodoraturn' volatile group; they had 29% genetic similarity to race 4 isolates and 76% similarity to race 1 isolates based on RAPD-PCR banding patterns; they belonged in the same pectic zymogram group as race 1 isolates and were virulent on 3-month-old Cavendish cv. Williams, Gros Michel and Pisang Gajih Merah plants in glasshouse tests.

scholarly journals Identification and Characterization of circRNAs Responsive to Methyl Jasmonate in Arabidopsis thaliana

2020 ◽  
Vol 21 (3) ◽  
pp. 792 ◽  
Author(s):  
Jingjing Zhang ◽  
Ruiqi Liu ◽  
Yanfeng Zhu ◽  
Jiaxin Gong ◽  
Shuwei Yin ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
High Throughput Sequencing ◽  
Enrichment Analysis ◽  
Pcr Analysis ◽  
Circular Rnas ◽  
Go Enrichment ◽  
Meja Treatment ◽  
Regulation Network ◽  
Almost All

Circular RNAs (circRNAs) are endogenous noncoding RNAs with covalently closed continuous loop structures that are formed by 3′–5′ ligation during splicing. These molecules are involved in diverse physiological and developmental processes in eukaryotic cells. Jasmonic acid (JA) is a critical hormonal regulator of plant growth and defense. However, the roles of circRNAs in the JA regulatory network are unclear. In this study, we performed high-throughput sequencing of Arabidopsis thaliana at 24 h, 48 h, and 96 h after methyl JA (MeJA) treatment. A total of 8588 circRNAs, which were distributed on almost all chromosomes, were identified, and the majority of circRNAs had lengths between 200 and 800 bp. We identified 385 differentially expressed circRNAs (DEcircRNAs) by comparing data between MeJA-treated and untreated samples. Gene Ontology (GO) enrichment analysis of the host genes that produced the DEcircRNAs showed that the DEcircRNAs are mainly involved in response to stimulation and metabolism. Additionally, some DEcircRNAs were predicted to act as miRNA decoys. Eight DEcircRNAs were validated by qRT-PCR with divergent primers, and the junction sites of five DEcircRNAs were validated by PCR analysis and Sanger sequencing. Our results provide insight into the potential roles of circRNAs in the MeJA regulation network.

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

Characterization of Partial Gene Deletions in Type III von Willebrand Disease with Alloantibody Inhibitors

1994 ◽  
Vol 72 (02) ◽  
pp. 180-185 ◽  
Author(s):  
David J Mancuso ◽  
Elodee A Tuley ◽  
Ricardo Castillo ◽  
Norma de Bosch ◽  
Pler M Mannucci ◽  
...  
Keyword(s):  
Von Willebrand Factor ◽  
Von Willebrand Disease ◽  
Pcr Analysis ◽  
Gene Deletions ◽  
Factor Gene ◽  
Type Iii ◽  
Large Deletions ◽  
Von Willebrand ◽  
Willebrand Factor

Summaryvon Willebrand factor gene deletions were characterized in four patients with severe type III von Willebrand disease and alloantibodies to von Willebrand factor. A PCR-based strategy was used to characterize the boundaries of the deletions. Identical 30 kb von Willebrand factor gene deletions which include exons 33 through 38 were identified in two siblings of one family by this method. A small 5 base pair insertion (CCTGG) was sequenced at the deletion breakpoint. PCR analysis was used to detect the deletion in three generations of the family, including two family members who are heterozygous for the deletion. In a second family, two type III vWD patients, who are distant cousins, share an -56 kb deletion of exons 22 through 43. The identification and characterization of large vWF gene deletions in these type III vWD patients provides further support for the association between large deletions in both von Willebrand factor alleles and the development of inhibitory alloantibodies.

Acanthamoeba Species in Tap Water, Egypt

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Ahmad Z Al-Herrawy ◽  
Mohamed A Marouf ◽  
Mahmoud A. Gad
Keyword(s):  
Water Samples ◽  
Tap Water ◽  
Pcr Analysis ◽  
Pulmonary Infections ◽  
Clinical Syndromes ◽  
Acanthamoeba Species ◽  
Amebic Encephalitis ◽  
Acanthamoeba Culbertsoni ◽  
Autumn And Winter

Genus Acanthamoeba causes 3 clinical syndromes amebic keratitis, granulomatous amebic encephalitis and disseminated granulomatous amebic disease (eg, sinus, skin and pulmonary infections). A total of 144 tap water samples were collected from Giza governorate, Egypt. Samples were processed for detection of Acanthamoeba species using non-nutrient agar (NNA) and were incubated at 30oC. The isolates of Acanthamoeba were identified to species level based on the morphologic criteria. Molecular characterization of the Acanthamoeba isolates to genus level was performed by using PCR. The obtained results showed that the highest occurrence percentage of Acanthamoeba species in water samples was observed in summer season (38.9%), then it decreased to be 30.6% in spring and 25% in each of autumn and winter. PCR analysis showed that 100% of 43 Acanthamoeba morphologically positive samples were positive by genus specific primer. In the present study eight species of Acanthamoeba can be morphologically recognized namely Acanthamoeba triangularis, Acanthamoeba echinulata, Acanthamoeba astronyxis, Acanthamoeba comandoni, Acanthamoeba griffini, Acanthamoeba culbertsoni, Acanthamoeba quina and Acanthamoeba lenticulata. In conclusion, the most common Acanthamoeba species in tap water was Acanthamoeba comandoni

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