Cryphodera sinensis n. sp. (Nematoda: Heteroderidae), a non-cyst-forming parasitic nematode from the root of ramie Boehmeria nivea in China

2013 ◽  
Vol 88 (4) ◽  
pp. 468-480 ◽  
Author(s):  
K. Zhuo ◽  
H.H. Wang ◽  
W. Ye ◽  
D.L. Peng ◽  
J.L. Liao
Keyword(s):  
Internal Transcribed Spacer ◽  
Parasitic Nematode ◽  
Large Subunit ◽  
Small Subunit ◽  
Cyst Nematodes ◽  
Lateral Field ◽  
Second Stage ◽  
Boehmeria Nivea ◽  
Rdna Sequences ◽  
Rrna Sequences

AbstractCryphodera sinensis n. sp. is described from ramie (Boehmeria nivea) based on the morphology and molecular analyses of rRNA small subunit (SSU), D2D3 expansion domains of large subunit (LSU D2D3) and internal transcribed spacer (ITS). This new species is characterized by oval females with a distinct subcrystalline layer and pronounced and protruding vulval lip, distinctly concave vulva–anus profile and a vulva–anus distance of 29.5–35.8 μm. Males possess two annuli in the lip region, a stylet 27–32.5 μm in length with round knobs sloping slightly posteriorly, lateral fields with three lines, spicules 20–28 μm long and the presence of a short cloacal tube. Second-stage juveniles possess three lip annuli, a stylet 28–31 μm in length with well-developed knobs projected anteriorly and three lines along the lateral field. The pointed tail, 52–65 μm long, possesses a mucro-like tip and a hyaline region, 24.5–35 μm long. Large phasmids with a lens-like structure are located 2–6 annuli posterior to the anus. Phylogenetic analysis shows that the species has unique SSU, LSU D2D3 and ITS rRNA sequences. Phylogenetic relationships of the three rDNA sequences of C. sinensis n. sp. and other cystoid/cyst nematodes are analysed together with a comparison of other species within the genus Cryphodera.

On the phylogeny of Ophiostoma, Ceratocystis s.s., and Microascus, and relationships within Ophiostoma based on partial ribosomal DNA sequences

1993 ◽  
Vol 71 (9) ◽  
pp. 1249-1265 ◽  
Author(s):  
G. Hausner ◽  
J. Reid ◽  
G. R. Klassen
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Large Subunit ◽  
Small Subunit ◽  
Data Set ◽  
Rdna Sequences ◽  
Morphological Criteria ◽  
Small Subunit Rdna ◽  
Rdna Sequence Data ◽  
Large Subunit Rdna

Phylogenetic analysis of partial rDNA sequences suggests that Ophiostoma should remain the sole genus of the Ophiostomataceae, and this should be the sole family within the Ophiostomatales, whereas Ceratocystis s.s. would be best disposed within the Microascales. Although morphological criteria suggest that the genus Ophiostoma is heterogeneous, analysis of partial small subunit rDNA sequence data shows that Ophiostoma (excluding O. roraimense) represents a monophyletic taxon. Analysis of a partial large subunit rDNA data set, which included sequences from 55 species assignable to Ophiostoma, failed to support the strict subdivision of the genus based on either ascospore characters or the nature of the anamorph. Key words: Ceratocystis, Microascus, Ophiostoma, partial rDNA sequences, phylogeny.

Observations on Malenchus geraerti n. sp. (Rhabditida: Tylenchidae), a morphological and molecular phylogenetic study

Zootaxa ◽  
2018 ◽  
Vol 4369 (3) ◽  
pp. 406 ◽  
Author(s):  
MAJID PEDRAM ◽  
MAHYA SOLEYMANZADEH ◽  
EBRAHIM POURJAM ◽  
MAHYAR MOBASSERI
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Phylogenetic Study ◽  
Molecular Characteristics ◽  
Lsu Rdna ◽  
Northern Iran ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Short Body

Malenchus geraerti n. sp., recovered from natural regions of northern Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having females with a short body, an anteriorly wide S-shaped amphidial opening narrowing posteriorly, cuticle with prominent annuli, lateral field a plain band with smooth margins, muscular metacorpus with well-developed valve and corresponding plates, spermatheca filled with small spheroid sperm cells, vulva sunken in body with large epiptygma and no flap, and conical tail tapering gradually to a more or less pointed tip. Males of the new species are characterized by having a short body, tylenchoid spicules, adcloacal bursa with smooth margin and tail similar to that of the female. Morphologically, the new species is similar to five known species of the genus: M. fusiformis, M. machadoi, M. pachycephalus, M. solovjovae and M. undulatus. It most closely resembles M. pachycephalus, but as a cryptic species it can be differentiated using morphological and molecular characteristics. Comparisons with the four other aforementioned species are also discussed. Molecular phylogenetic studies using partial sequences of small and large subunit ribosomal DNA fragments reveal that the new species forms a clade with the species M. neosulcus in the small subunit (SSU) rDNA, and two species of Lelenchus in the large subunit (LSU) rDNA tree. 

On the morphology and phylogeny of Robustodorus Andrássy, 2007 and two ‘Aphelenchoides’ species (Nematoda: Aphelenchoidinae)

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 601-615
Author(s):  
Natsumi Kanzaki ◽  
Ebrahim Shokoohi ◽  
Hendrika Fourie ◽  
Antoinette Swart ◽  
Loureine Muller ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
Irrigation Scheme ◽  
Large Numbers ◽  
Molecular Studies ◽  
Garlic Bulb ◽  
Northern Cape ◽  
Production Areas

Summary In a survey of nematode damage in groundnut (Arachis hypogaea) (Fabaceae) production areas in the Vaalharts Irrigation Scheme in the Northern Cape Province of South Africa, a nematode was recovered and initially believed to be a member of the genus Robustodorus, i.e., the species has a characteristic robust stylet with very well-developed teardrop-shaped (garlic bulb-like) basal swellings. The specimens were recovered in large numbers from damaged hulls and kernels of field-collected groundnuts and, after thorough morphological and molecular studies, were subsequently identified as Aphelenchoides arachidis. Molecular analysis based on near-full-length small subunit (SSU), internal transcribed spacer (ITS) and D2-D3 expansion segments of the large subunit (LSU) rRNA genes supported the monophyly of A. arachidis, A. subtenuis and R. megadorus within the subfamily Aphelenchoidinae. Based on the typological characters observed in the present study compared with descriptions in the literature, these three species are considered as congeneric, i.e., they share very well-developed basal swellings of the stylet. Accordingly, these two Aphelenchoides species are transferred to Robustodorus as R. arachidis n. comb. and R. subtenuis n. comb. In addition, the male tail characters of Robustodorus and Aphelenchoides are discussed.

Molecular Phylogenetics of Ramaria and Related Genera: Evidence from Nuclear Large Subunit and Mitochondrial Small Subunit rDNA Sequences

Mycologia ◽  
2001 ◽  
Vol 93 (3) ◽  
pp. 465 ◽  
Author(s):  
Andrea J. Humpert ◽  
Eric L. Muench ◽  
Admir J. Giachini ◽  
Michael A. Castellano ◽  
Joseph W. Spatafora
Keyword(s):  
Molecular Phylogenetics ◽  
Large Subunit ◽  
Small Subunit ◽  
Rdna Sequences ◽  
Small Subunit Rdna

Molecular and morphological characterisation of Paurodontella persica n. sp. (Hexatylina: Sphaerulariidae) from soil in Iran

Nematology ◽  
2017 ◽  
Vol 19 (1) ◽  
pp. 57-68 ◽  
Author(s):  
Mehrab Esmaeili ◽  
Ramin Heydari ◽  
Weimin Ye
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Small Subunit ◽  
Body Contour ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Rdna Gene ◽  
Posterior Extension ◽  
Large Subunit Rdna ◽  
A New Species

A new species ofPaurodontella,P. persican. sp., collected from the Alborz rangelands of Iran, is described and illustrated. The new species is characterised by its body length of 506 (416-599) μm and 424 (388-461) μm in female and male, respectively, four incisures in lateral field, lip region annulated and continuous with body contour, total stylet 6.2 (5.5-7.0) μm long. Basal pharyngeal bulb with small posterior extension projecting reaching to the intestine. Excretory pore situated at the level of basal pharyngeal bulb region. Post-uterine sac short, 4.5 (3.0-6.0) μm long and uterus with an offset diverticulum. Tail of both sexes similar, conoid pointed, terminus tapering into a long pointed mucron. Male with adanal bursa and spicules 16.5 (16.0-17.0) μm long. The new species comes close in morphology and morphometrics to four known species of the genus, namelyP. auriculata,P. myceliophaga,P. minutaandP. niger. Molecular analyses of the partial small subunit rDNA gene (SSU), D2-D3 expansion segments of the large subunit rDNA gene (LSU) and internal transcribed spacer (ITS) revealed this to be a new species.

Discovery of a free-living nematode phylogenetically related to vertebrate parasites of the genus Strongyloides (Nematoda : Strongyloidoidea): morphological, anatomical and molecular characterisation

2016 ◽  
Vol 30 (4) ◽  
pp. 387
Author(s):  
Ren-E Huang ◽  
Runsheng Li ◽  
Zhongying Zhao
Keyword(s):  
Culture Media ◽  
Large Subunit ◽  
Molecular Genetic ◽  
Small Subunit ◽  
Land Snails ◽  
Rrna Genes ◽  
Lsu Rdna ◽  
Free Living ◽  
Rdna Sequences ◽  
Free Living Nematode

Nematodes are important, largely because they frequently act as parasites and threaten the health of plants, animals and even humans. Here, we describe an interesting free-living nematode from land snails on Luofu Mountain, Guangdong, China. Alloionema luofuensis, sp. nov. is phylogenetically related to slug-parasite A. appendiculatum and the well-known vertebrate parasites Strongyloides spp. based on small subunit (SSU) and the D2-D3 domain of large subunit (LSU) rDNA sequences. The new species possesses an extremely transparent body and is easily maintained using C. elegans culture media, suggesting a possible application prospect of this free-living nematode as a comparative model system for its related parasites. Morphology and anatomy of the gonochoristic A. luofuensis, sp. nov. adult were described and illustrated. The species is characterised by a filiform tail bisexually, ‘rhabditiform’ oesophagus and ‘rhabditid-like’ female anatomy, but its male caudal region is completely different from that of typical rhabditid nematodes, being absent from an enveloping bursa. It is the first marker taxon characterised morphologically as well as molecularly from the family Alloionematidae, a group of nematodes with hyperdiverse molecular genetic variations underlying highly conserved anatomy. Further molecular and genetic studies on A. luofuensis, sp. nov. populations hold promise to provide insight into evolution of the clade consisting of vertebrate parasites of the heterogonic nematode genus Strongyloides. This is because of its unusual high levels of heterozygosity maintained by the conserved rRNA genes of partial SSU and the D2-D3 domain of LSU for the type isolate of this species.

Phylogenetic analysis of Rhizoclonium (Cladophoraceae, Cladophorales), and the description of Rhizoclonium subtile sp. nov. from China

Phytotaxa ◽  
2018 ◽  
Vol 383 (2) ◽  
pp. 147 ◽  
Author(s):  
ZHI-JUAN ZHAO ◽  
HUAN ZHU ◽  
GUO-XIANG LIU ◽  
ZHENG-YU HU
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Nuclear Gene ◽  
Morphological Characters ◽  
Small Subunit ◽  
Molecular Evidence ◽  
Gene Markers ◽  
Long Time ◽  
Small Subunit Ribosomal Dna

The genus Rhizoclonium (Cladophoraceae, Cladophorales) accommodates uniserial, unbranched filamentous algae, closely related to Cladophora and Chaetomorpha. Its taxonomy has been problematic for a long time due to the lack of diagnostic morphological characters. To clarify the species diversity and taxonomic relationships of this genus, we collected and analyzed thirteen freshwater Rhizoclonium specimens from China. The morphological traits of these specimens were observed and described in detail. Three nuclear gene markers small subunit ribosomal DNA (SSU), large subunit ribosomal DNA (LSU) and internal transcribed spacer 2 (ITS2) sequences were analyzed to elucidate their phylogenetic relationships. The results revealed that there were at least fifteen molecular species assignable to Rhizoclonium and our thirteen specimens were distributed in four clades. On the basis of morphological and molecular evidence we propose the new species, R. subtile sp. nov.

scholarly journals Isolation and Characterization of a Novel Facultative Anaerobic Filamentous Fungus from Japanese Rice Field Soil

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Akio Tonouchi
Keyword(s):  
Filamentous Fungus ◽  
Rice Field ◽  
Large Subunit ◽  
Small Subunit ◽  
Lsu Rdna ◽  
Field Soil ◽  
Rice Field Soil ◽  
Rdna Sequences ◽  
Isolation And Characterization

A novel filamentous fungus strain designated RB-1 was isolated into pure culture from Japanese rice field soil through an anaerobic role tube technique. The strain is a mitosporic fungus that grows in both aerobic and strict anaerobic conditions using various mono-, di-, tri-, and polysaccharides with acetate and ethanol productions. The amount of acetate produced was higher than that of ethanol in both aerobic and anaerobic cultures. The characteristic verrucose or punctuate conidia of RB-1 closely resembled those of some strains of the genusThermomyces, a thermophilic or mesophilic anamorphic ascomycete. However, based on phylogenetic analysis with the small subunit (SSU) and large subunit (LSU) rDNA sequences, RB-1 was characterized as a member of the class Lecanoromycetes of the phylum Ascomycota. Currently, RB-1 is designated as an anamorphic ascomycete and is phylogenetically considered anincertae sediswithin the class Lecanoromycetes.

Heterodera circeae sp. n. and H. scutellariae sp. n. (Tylenchida: Heteroderidae) from Germany, with notes on the goettingiana group

Nematology ◽  
2004 ◽  
Vol 6 (3) ◽  
pp. 343-355 ◽  
Author(s):  
Sergei Subbotin ◽  
Dieter Sturhan
Keyword(s):  
New Species ◽  
Tail Length ◽  
Molecular Data ◽  
The Other ◽  
Phylogenetic Position ◽  
Lateral Field ◽  
Second Stage ◽  
Rdna Sequences ◽  
Dicotyledonous Plants ◽  
Stylet Length

Abstract Two species of cyst-forming nematodes belonging to the goettingiana group are described from woodland in Germany. Heterodera circeae sp. n., parasitising Circaea lutetiana (Onagraceae), most closely resembles H. carotae and H. cruciferae, from which it differs by larger cysts (430-720 μm) and longer fenestral length (27-54 μm) and rounded stylet knobs of the second-stage juveniles. Heterodera scutellariae sp. n., found on the labiate Scutellaria galericulata (Lamiaceae), is distinguished from the other species of this group by shorter body (358-437 μm) and tail (41-53 μm) of the second-stage juveniles. The ITS-rDNA sequences of the new species are very similar and differ from each other by only a few nucleotides. The phylogenetic position of the new species within the group is presented based on analyses of molecular data. A revised diagnosis of the goettingiana group is proposed. The species of the group have lemon-shaped, ambifenestrate and abullate (small bullae occasionally present) cysts with egg sac, long vulval slit (> 35 μm), thin vulval bridge, low semifenestrae (average fenestral length 30-45 μm), and weak underbridge. Second-stage juveniles are characterised by body length > 400 μm, tail length > 45 μ m, hyaline tail portion > 20 μm, stylet length > 20 μm, lateral field with four incisures and small phasmids. All known species of the goettingiana group exclusively parasitise dicotyledonous plants.

Sign in / Sign up

Export Citation Format

Share Document