Mean nuclear area and chromosomal DNA content of squamous cell carcinomas of the oral cavity using computerized image analysis

1997 ◽  
Vol 111 (2) ◽  
pp. 141-144 ◽  
Author(s):  
S. K. Sarker ◽  
K. S. Patel
Keyword(s):  
Image Analysis ◽  
Oral Cavity ◽  
Dna Content ◽  
Squamous Cell Carcinomas ◽  
Nuclear Area ◽  
Chromosomal Dna ◽  
Node Negative ◽  
Computerized Image Analysis ◽  
Node Positive ◽  
Chromosomal Dna Content

AbstractMorphometric measurements of nuclei may be of prognostic value in some cancers. In this present study we have evaluated the mean nuclear area (MNA) of 50 squamous cell carcinomas of the oral cavity (SCCOC) using computerized image analysis. Since chromosomal DNA content is a reflection of the DNA content in the nucleus, we have evaluated the relationship between MNA and chromosomal DNA. Thirteen tumours had a MNA greater than 49.9 μm2 and 37 had a MNA less than this. Six tumours were classified as hypodiploid, 29 as diploid and 15 as aneuploid. There were 44 node-negative patients and six node-positive. When comparing MNA in these groups, 50 per cent of node-positive patients had a larger MNA whilst only 20 per cent of the node-negative group had a large MNA. The correlation coefficient between MNA and DNA indices was r = 0.75. The greater nuclear size is possibly a reflection of a more aggressive tumour biology in the node-positive patients. We conclude that a large MNA may be a marker of aggressive tumour biology in this group. In the future, we aim to evaluate the prognostic significance of MNA in patients with SCCOC.

scholarly journals Proposing prognostic thresholds for lymph node yield in clinically lymph node-negative and lymph node-positive cancers of the oral cavity

Cancer ◽  
2016 ◽  
Vol 122 (23) ◽  
pp. 3624-3631 ◽  
Author(s):  
Phoebe Kuo ◽  
Saral Mehra ◽  
Julie A. Sosa ◽  
Sanziana A. Roman ◽  
Zain A. Husain ◽  
...  
Keyword(s):  
Lymph Node ◽  
Oral Cavity ◽  
Lymph Node Yield ◽  
Node Negative ◽  
Node Positive ◽  
Lymph Node Positive ◽  
Node Yield

scholarly journals Comparison of the Coffea canephora and C. arabica karyotype based on chromosomal DNA content

2008 ◽  
Vol 28 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Wellington Ronildo Clarindo ◽  
Carlos Roberto Carvalho
Keyword(s):  
Dna Content ◽  
Coffea Canephora ◽  
Chromosomal Dna ◽  
Chromosomal Dna Content

scholarly journals Regional Failures after Selective Neck Dissection in Previously Untreated Squamous Cell Carcinoma of Oral Cavity

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Hassan Iqbal ◽  
Abu Bakar Hafeez Bhatti ◽  
Raza Hussain ◽  
Arif Jamshed
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cavity ◽  
Cell Carcinoma ◽  
Neck Dissection ◽  
Squamous Cell ◽  
Treatment Modalities ◽  
Selective Neck Dissection ◽  
Node Negative ◽  
Node Positive ◽  
Regional Control

Aim. To share experience with regional failures after selective neck dissection in both node negative and positive previously untreated patients diagnosed with squamous cell carcinoma of the oral cavity.Patients and Methods. Data of 219 patients who underwent SND at Shaukat Khanum Cancer Hospital from 2003 to 2010 were retrospectively reviewed. Patient characteristics, treatment modalities, and regional failures were assessed. Expected 5-year regional control was calculated and prognostic factors were determined.Results. Median follow-up was 29 (9–109) months. Common sites were anterior tongue in 159 and buccal mucosa in 22 patients. Pathological nodal stage was N0 in 114, N1 in 32, N2b in 67, and N2c in 5 patients. Fourteen (6%) patients failed in clinically node negative neck while 8 (4%) failed in clinically node positive patients. Out of 22 total regional failures, primary tumor origin was from tongue in 16 (73%) patients. Expected 5-year regional control was 95% and 81% for N0 and N+ disease, respectively (P<0.0001). Only 13% patients with well differentiated, T1 tumors in cN0 neck were pathologically node positive.Conclusions. Selective neck dissection yields acceptable results for regional management of oral squamous cell carcinoma. Wait and see policy may be effective in a selected subgroup of patients.

scholarly journals Chromosomal DNA content of sweet pepper determined by association of cytogenetic and cytometric tools

2008 ◽  
Vol 27 (7) ◽  
pp. 1227-1233 ◽  
Author(s):  
Isabella Santiago de Abreu ◽  
Carlos Roberto Carvalho ◽  
Wellington Ronildo Clarindo
Keyword(s):  
Dna Content ◽  
Sweet Pepper ◽  
Chromosomal Dna ◽  
Chromosomal Dna Content

scholarly journals Genotoxicity Assessment of Potassium Bromate by Means of DNA Image Analysis on the Root Tip Nuclei of Allium sativum L.

2020 ◽  
pp. 9-14
Author(s):  
Hoda, A. Khatab ◽  
Nagat, S. Elhaddad ◽  
Samia Eissa
Keyword(s):  
Image Analysis ◽  
Dna Content ◽  
Allium Sativum ◽  
Food Additives ◽  
Genotoxic Effect ◽  
Root Tip ◽  
Dependent Manner ◽  
Chromosomal Dna ◽  
Efficient Test ◽  
Dna Image Analysis

Allium sativum assay was widely used to assess the compound's cytotoxicity and genotoxicity on plants and animals. Only few studies analyzed the genotoxic effect of potassium bromated (KBrO3) on the DNA content of plant cells. DNA content assay is an efficient test for the measure of chromosomal DNA damages. Based on this approach the effect of KBrO3 on DNA content change was investigated in root tip nuclei of A. sativum. Different concentrations of food additives KBrO3. 3 g/l, 5 g/l 7 g/l and 9 g/l % were prepared and treatments were given. A. sativum root were incubated for 2, 6 and 24 hours and DNA image analysis of root tip nuclei was performed. The analysis was based on the measurement of the Mean Optical Density (MOD) which represents the cellular DNA content. The results showed that the KBrO3 significantly decreased of DNA content compared to the control at all concentrations and treatment periods in dose-dependent manner. The present study suggests that extensive use of food additives should be banned due to genotoxic effect on living cells. Therefore, there is an urgent need to evaluate potential mutagenic effects of KBrO3 on human.

scholarly journals An improved, non-isotopic method of screening cells from patients with abnormalities of sexual differentiation for Y chromosomal DNA content.

1993 ◽  
Vol 30 (4) ◽  
pp. 304-307 ◽  
Author(s):  
M Witt ◽  
K Michalczak ◽  
A Latos-Bielenska ◽  
J Jaruzelska ◽  
I Kuczora ◽  
...  
Keyword(s):  
Dna Content ◽  
Sexual Differentiation ◽  
Chromosomal Dna ◽  
Isotopic Method ◽  
Chromosomal Dna Content
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