Autologous fat injection therapy including a high concentration of adipose-derived regenerative cells in a vocal fold paralysis model: animal pilot study

2016 ◽  
Vol 130 (10) ◽  
pp. 914-922 ◽  
Author(s):  
N Nishio ◽  
Y Fujimoto ◽  
K Suga ◽  
Y Iwata ◽  
K Toriyama ◽  
...  
Keyword(s):  
Vocal Fold ◽  
Vocal Fold Paralysis ◽  
Injection Therapy ◽  
Unilateral Vocal Fold Paralysis ◽  
Fat Injection ◽  
High Concentration ◽  
Autologous Fat ◽  
Regenerative Cells ◽  
The Right ◽  
Thyroarytenoid Muscle

AbstractObjectives:To verify the effectiveness and safety of the addition of adipose-derived regenerative cells to autologous fat injection therapy.Methods:Unilateral vocal fold paralysis models were made by cutting the right recurrent laryngeal nerve in two pigs. At day 30, 0.5 ml adipose-derived regenerative cells mixed with 1 ml autologous fat was injected into the right vocal fold of one pig, with the other receiving 0.5 ml Ringer's solution mixed with 1 ml autologous fat. At day 120, fibrescopy, laser Doppler flowmeter, computed tomography, vocal function evaluation and histological assessment were conducted.Results:Although histological assessment revealed atrophy of the thyroarytenoid muscle fibre in both pigs, there was remarkable hypertrophy of the thyroarytenoid muscle fibre in the area surrounding the adipose-derived regenerative cells injection site.Conclusion:The addition of a high concentration of adipose-derived regenerative cells to autologous fat injection therapy has the potential to improve the treatment outcome for unilateral vocal fold paralysis.

Autologous Fat Injection for Vocal Fold Paralysis: Long-Term Histologic Evaluation

1995 ◽  
Vol 104 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Lauren S. Zaretsky ◽  
Michael deTar ◽  
Maisie L. Shindo ◽  
Dale H. Rice
Keyword(s):  
Vocal Fold ◽  
Vocal Fold Paralysis ◽  
Laryngeal Nerve ◽  
Fat Injection ◽  
Autologous Fat ◽  
Alloplastic Materials ◽  
Histologic Evaluation ◽  
Left Recurrent Laryngeal Nerve ◽  
Thyroarytenoid Muscle

Many techniques have been developed for medialization of the paralyzed vocal fold. The purpose of this study is to evaluate autologous fat as an alternative to alloplastic substances for use in vocal fold medialization. Eight dogs underwent left recurrent laryngeal nerve sectioning. Autologous fat was harvested, and the paralyzed vocal fold was medialized by injecting the fat into the thyroarytenoid muscle. The animals were divided into three groups for evaluation at 1, 3, and 6 months. Videolaryngoscopy was performed prior to sacrificing the animals. The larynges were sectioned coronally, and histologic studies were performed. The studies confirmed the preservation of viable fat at the injected site in all animals. Only a minimal inflammatory response was observed in the 1-month group. It would appear that fat injection is a viable alternative to Teflon injection and thyroplasty; it eliminates the need for alloplastic materials, does not appear to migrate, and does not require an open procedure.

Comparative study of framework surgery and fat injection laryngoplasty

2009 ◽  
Vol 123 (S31) ◽  
pp. 35-41 ◽  
Author(s):  
H Umeno ◽  
S Chitose ◽  
K Sato ◽  
T Nakashima
Keyword(s):  
Comparative Study ◽  
Vocal Fold ◽  
Acoustic Analysis ◽  
Functional Results ◽  
Vocal Fold Paralysis ◽  
Injection Laryngoplasty ◽  
Unilateral Vocal Fold Paralysis ◽  
Fat Injection ◽  
Autologous Fat ◽  
Before And After

AbstractObjective:To evaluate differences between the functional results of framework surgery and autologous fat injection laryngoplasty, for patients with unilateral vocal fold paralysis.Study design:Sixty-two patients underwent framework surgery, while 64 received autologous fat injection laryngoplasty. Voice function before and after both procedures was assessed using aerodynamic and acoustic analysis, with differences evaluated using paired t-test in both groups.Results:In both groups, all parameters improved significantly after surgery, compared with before surgery. Post-operative improvement in all parameters was significantly greater after fat injection laryngoplasty, compared with framework surgery.Conclusion:Autologous fat injection laryngoplasty was thus found to be a more effective and reliable therapy for improving voice function in patients with vocal fold paralysis, compared with framework surgery.

Autologous Fat Injection for Unilateral Vocal Fold Paralysis

1996 ◽  
Vol 105 (8) ◽  
pp. 602-606 ◽  
Author(s):  
Maisie L. Shindo ◽  
Lauren S. Zaretsky ◽  
Dale H. Rice
Keyword(s):  
Vocal Fold ◽  
Voice Quality ◽  
Vocal Fold Paralysis ◽  
Unilateral Vocal Fold Paralysis ◽  
Fat Injection ◽  
Autologous Fat ◽  
The Voice ◽  
Fat Volume

This study evaluates the efficacy of autologous fat injection for medialization of the paralyzed vocal fold. In 21 patients with unilateral vocal fold paralysis, autologous abdominal fat was injected into the thyroarytenoid muscle to achieve medialization. All patients were followed up with serial videolaryngoscopy and voice evaluation. At 2 months' follow-up, the voice was judged to be excellent in 10 patients, slightly breathy but significantly better than the preoperative voice in 6 patients, and markedly breathy in 4 patients. At 3 to 4 months' follow-up, of the 10 patients with excellent results, 5 maintained an excellent voice, 3 had developed slight breathiness, and 1 had developed severe breathiness. Long-term (6 to 12 months) results were available in 11 patients, and all of them maintained the same voice quality that was noted during the 3 to 4 months' examination. Magnetic resonance imaging of the larynx was obtained in 7 patients at intervals ranging from 1 to 7 months and compared to the baseline scan obtained at 1 week postoperative to assess the amount of fat remaining in the muscle. The images showed fat volume to persist, but a decrease in the fat signal was observed over time. The results suggest that the duration of medialization with autologous fat is variable, but appears to last at least 2 to 3 months. This loss of volume after 3 months seems to be due to absorption of the fat and possibly muscle atrophy. Autologous fat injection is relatively safe and easy to perform, and is an ideal method of temporary vocal fold medialization in patients in whom return of vocal fold function is expected.

Long-term histological outcomes of injected autologous fat into human vocal folds after secondary laryngectomy

2005 ◽  
Vol 132 (5) ◽  
pp. 685-688 ◽  
Author(s):  
Hisanori Sasai ◽  
Yusuke Watanabe ◽  
Hiroshi Muta ◽  
Junichi Yoshida ◽  
Ibuki Hayashi ◽  
...  
Keyword(s):  
Vocal Fold ◽  
Vocal Folds ◽  
Vocal Fold Paralysis ◽  
Histological Evaluation ◽  
Fat Injection ◽  
Special Equipment ◽  
Autologous Fat ◽  
Vocal Fold Augmentation ◽  
Disposable Needle

OBJECTIVE: To histologically evaluate the long-term outcomes of autologous fat grafts after injection laryngoplasty in the human larynx. STUDY DESIGN AND SETTING: We injected liposuctioned fat for vocal fold augmentation in patients with vocal fold paralysis. We suctioned autologous fat from the low abdomen with an 18-G disposable needle and a 20-mL disposable syringe under negative pressure. This is different from the conventional liposuction technique and avoids the use of special equipment. In this article, we report the histological evaluation of 2 patients (patient 1: 12 months, patient 2: 41 months) who required total laryngectomy after autologous fat injection into the vocal folds. RESULTS: Histological examination revealed normal-appearing viable adipocytes with minimal inflammatory response in both patients. CONCLUSIONS: Our liposuctioned autologous fat injection histologically offered long-term improvement in patients with impaired glottal closure from vocal fold paralysis. (Otolaryngol Head Neck Surg 2005;132:685-688.)

Histologic Study of an Autologous Fat Graft in the Larynx of Dogs With Unilateral Vocal Fold Paralysis

2007 ◽  
Vol 117 (11) ◽  
pp. 2045-2049 ◽  
Author(s):  
Leonardo de Souza Kruschewsky ◽  
Francisco Verissimo de Mello-Filho ◽  
Fabiano Saggioro ◽  
Luciano Neder Serafini ◽  
Clark Allan Rosen
Keyword(s):  
Vocal Fold ◽  
Vocal Fold Paralysis ◽  
Fat Graft ◽  
Unilateral Vocal Fold Paralysis ◽  
Histologic Study ◽  
Autologous Fat ◽  
Autologous Fat Graft

scholarly journals Injection Laryngoplasty Using Autologous Fat Enriched with Adipose-Derived Regenerative Stem Cells: A Safe Therapeutic Option for the Functional Reconstruction of the Glottal Gap after Unilateral Vocal Fold Paralysis

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
José M. Lasso ◽  
Daniel Poletti ◽  
Batolomé Scola ◽  
Pedro Gómez-Vilda ◽  
Ana I. García-Martín ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Phase I ◽  
Vocal Fold ◽  
Vocal Fold Paralysis ◽  
Unilateral Vocal Fold Paralysis ◽  
Autologous Fat ◽  
Functional Reconstruction ◽  
Fat Grafts ◽  
Group I ◽  
Group Ii

Background. Paralysis of one vocal fold leads to glottal gap and vocal fold insufficiency that has significant impact upon a patient’s quality of life. Fillers have been tested to perform intracordal injections, but they do not provide perdurable results. Early data suggest that enriching fat grafts with adipose-derived regenerative cells (ADRCs) promote angiogenesis and modulate the immune response, improving graft survival. The aim of this study is to propose ADRC-enriched adipose tissue grafts as effective filler for the paralyzed vocal fold to use it for functional reconstruction of the glottal gap. Method. This is the first phase I-IIA clinical trial (phase I/IIA clinical trial, unicentric, randomized, controlled, and two parallel groups), to evaluate the safety of a new therapy with ADRC-enriched fat grafting (ADRC: group I) for laryngoplasty after unilateral vocal fold paralysis. Control group patients received centrifuged autologous fat (CAF: group II) grafts. Overall mean age is 52.49 ± 16.60 years. Group I (ADRC): 7 patients (3 males and 4 females), 52.28 ± 20.95 year. Group II (CAF): 7 patients (3 males and 4 females), 52.71 ± 12.59 year. Results. VHI-10 test showed that preoperative mean score was 24.21 ± 8.28. Postoperative mean score was 6.71 ± 6.75. Preoperative result in group I was 21.14 ± 3.58 and postoperative result was 3.14 ± 3.53. Preoperative result for group II was 27.29 ± 10.66. Postoperative score in group II was 10.29 ± 7.52. Wilcoxon and the Student t-tests showed that the patient’s self-perception of posttreatment improvement is larger when ADRCs are used. Comparing pre- and posttreatment voice quality analysis, group I showed a p=0.053. Group II showed a p=0.007. There would be no significant differentiation between pre- and posttreatment results. This is true for group II and limited for group I. Conclusions. This prospective trial demonstrates the safety and efficacy of the treatment of glottal gap defects utilizing ADRC-enriched fat grafts. This trial is registered with NCT02904824.

Sign in / Sign up

Export Citation Format

Share Document