A morphological and histological investigation of the regeneration in Myxicola infundibulum (Montagu, 1808) (Sabellida, Annelida)

2017 ◽  
Vol 97 (5) ◽  
pp. 1155-1165 ◽  
Author(s):  
Nancy K. Prentiss ◽  
Mary S. Tyler ◽  
David Dean

Anterior regeneration of the annelid polychaete, Myxicola infundibulum (Montagu, 1808) is described from histological and SEM perspectives. This article provides additional evidence that anterior and posterior regeneration of isolated worm pieces does occur in this species, but that regenerative ability is restricted to abdominal pieces obtained from small individuals (less than 5 mm in thorax diameter and 10–20 mm in length). New cartilage tissue forms within the regenerating crown, but thoracic regeneration is limited to three segments. Anterior and posterior regeneration occurred within isolated pieces excised from the abdomen, resulting in the formation of 13 clones, with up to five individuals per clone.

Nature ◽  
2013 ◽  
Vol 500 (7460) ◽  
pp. 77-80 ◽  
Author(s):  
James M. Sikes ◽  
Phillip A. Newmark

2021 ◽  
Vol 57 (4) ◽  
pp. 166-180
Author(s):  
Maria-Minodora Marin ◽  
Madalina Georgiana Albu Kaya ◽  
George Mihail Vlasceanu ◽  
Jana Ghitman ◽  
Ionut Cristian Radu ◽  
...  

Type II collagen has been perceived as the indispensable element and plays a crucial role in cartilage tissue engineering. Thus, materials based on type II collagen have drawn farther attention in both academic and research for developing new systems for the cartilage regeneration. The disadvantage of using type II collagen as a biomaterial for tissue repairing is its reduced biomechanical properties. This can be solved by physical, enzymatic or chemical cross-linking processes, which provide biomaterials with the required mechanical properties for medical applications. To enhance type II collagen properties, crosslinked collagen scaffolds with different cross-linking agents were prepared by freeze-drying technique. The present research work studied the synthesis of type II collagen biomaterials with and without crosslinking agents. Scaffolds morphology was observed by MicroCT, showing in all cases an appropriate microstructure for biological applications, and the mechanical studies were performed using compressive tests. DSC showed an increase in denaturation temperature with an increase in cross-linking agent concentration. FTIR suggested that the secondary structure of collagen is not affected after the cross-linking; supplementary, to confirm the characteristic triple-helix conformation of collagen, the CD investigation was performed. The results showed that the physical-chemical properties of type II collagen were improved by cross-linking treatments.


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