Morphological observations and molecular confirmation of larvae of Levisquilla inermis (Crustacea: Stomatopoda) from the Seto Inland Sea

Abstract Mantis shrimps are commercially important crustaceans in many areas of the world. In contrast to the relatively common studies of adults, limited studies have been attempted of larvae because of a lack of identification keys. The objectives of this study were two-fold: (1) to link wild-caught larval specimens from the Seto Inland Sea to a certain species and (2) to present a detailed morphological description of the last larval stage. The resulting molecular phylogenetic tree based on 16S rRNA gene sequences strongly implies that our larval specimens were linked to Levisquilla inermis, which was barcoded from a newly collected adult specimen with a morphological identification. K2P genetic divergence was found to be 0% among the larval and adult specimens. The congeneric species L. jurichi was the sister group of the L. inermis cluster, but the K2P distance between them was 3.4%, and it was considered a distinct species. Morphological observations provided five major distinguishing characteristics of the last-stage larva of L. inermis: (1) propodus of second maxilliped with three basal spines, (2) all five pleopods possessing an appendix interna with cincinnuli, (3) exopod of uropod longer than the endopod, (4) distal margin of the exopod of the uropod reaching the lateral tooth of telson, and (5) telson with nine intermediate denticles. Overall, our results reveal the importance of combining molecular and morphological analyses for solving stomatopod larval identification. This finding can be used to support and facilitate future research on the taxonomy and biodiversity of stomatopod larvae.

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The Bacterial Community in the Gut of the Cockroach Shelfordella lateralis Reflects the Close Evolutionary Relatedness of Cockroaches and Termites

Applied and Environmental Microbiology ◽

10.1128/aem.07788-11 ◽

2012 ◽

Vol 78 (8) ◽

pp. 2758-2767 ◽

Cited By ~ 78

Author(s):

Christine Schauer ◽

Claire L. Thompson ◽

Andreas Brune

Keyword(s):

Bacterial Community ◽

Phylogenetic Analyses ◽

Rflp Analysis ◽

Sister Group ◽

Rrna Gene ◽

Content Type ◽

Evolutionary Relatedness ◽

Termite Gut ◽

Molecular Phylogenetic ◽

Metabolic Activities

ABSTRACTTermites and cockroaches are closely related, with molecular phylogenetic analyses even placing termites within the radiation of cockroaches. The intestinal tract of wood-feeding termites harbors a remarkably diverse microbial community that is essential for the digestion of lignocellulose. However, surprisingly little is known about the gut microbiota of their closest relatives, the omnivorous cockroaches. Here, we present a combined characterization of physiological parameters, metabolic activities, and bacterial microbiota in the gut ofShelfordella lateralis, a representative of the cockroach family Blattidae, the sister group of termites. We compared the bacterial communities within each gut compartment using terminal-restriction fragment length polymorphism (T-RFLP) analysis and made a 16S rRNA gene clone library of the microbiota in the colon—the dilated part of the hindgut with the highest density and diversity of bacteria. The colonic community was dominated by members of theBacteroidetes,Firmicutes(mainlyClostridia), and someDeltaproteobacteria. SpirochaetesandFibrobacteres, which are abundant members of termite gut communities, were conspicuously absent. Nevertheless, detailed phylogenetic analysis revealed that many of the clones from the cockroach colon clustered with sequences previously obtained from the termite gut, which indicated that the composition of the bacterial community reflects at least in part the phylogeny of the host.

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Molecular phylogenetic assessment of Spirobranchus kraussii-complex (Annelida: Serpulidae) from the Japanese Archipelago

PeerJ ◽

10.7717/peerj.11746 ◽

2021 ◽

Vol 9 ◽

pp. e11746

Author(s):

Genki Kobayashi ◽

Ryutaro Goto

Keyword(s):

Genetic Difference ◽

Phylogenetic Analyses ◽

Mitochondrial Gene ◽

Taxonomic Status ◽

Distinct Species ◽

Nucleotide Sequences ◽

The Other ◽

Its2 Region ◽

Rrna Gene ◽

Molecular Phylogenetic

Spirobranchus kraussii (Annelida: Serpulidae) was recognized as being widely distributed both in the Pacific and Atlantic Oceans. However, the sampling records far from its type locality (South Africa) have been questioned. Actually, recent molecular phylogenetic studies showed that S. kraussii contains genetically distinct species. In this study, we performed molecular phylogenetic analyses of S. cf. kraussii collected from Japan using the nucleotide sequences of a mitochondrial gene and two nuclear genes. Three lineages were recovered within Spirobranchus kraussii-complex in Japan, and one (Spirobranchus sp. 6) showed moderate genetic difference (approximately 4%) in the mitochondrial cytb gene sequence from Spirobranchus sp. 1, an undescribed sequenced species from Honshu Island, Japan. However, the nucleotide sequences of the 18S rRNA gene and ITS2 region were nearly indistinguishable. The other lineage was clearly distinct from the other previously sequenced species and is thus considered to be another distinct species of this species complex (Spirobranchus sp. 5). Although detailed morphological assessment of these lineages is necessary to define their taxonomic status, the present study provided further implications for the species diversity within the S. kraussii-complex.

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Conqueria laevis gen. and sp. nov., a new soft-walled, monothalamous foraminiferan from the deep Weddell Sea

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315404010197h ◽

2004 ◽

Vol 84 (5) ◽

pp. 919-924 ◽

Cited By ~ 23

Author(s):

Andrew J. Gooday ◽

Jan Pawlowski

Keyword(s):

Phylogenetic Analyses ◽

Sister Group ◽

Small Subunit ◽

Weddell Sea ◽

Rrna Gene ◽

Small Subunit Rrna ◽

Short Neck ◽

Small Subunit Rrna Gene ◽

Molecular Phylogenetic ◽

Cylindrical Test

Conqueria laevis gen. and sp. nov., a new monothalamous agglutinated foraminiferan, is described from core samples collected in the abyssal western Weddell Sea. The species is characterized by a very elongate, almost cylindrical test that usually follows a more or less curved course and has a single terminal aperture located at the end of a short neck. The wall has a very smooth outer surface and is composed of tiny (<5 μm) agglutinated particles. Very similar and presumably congeneric morphotypes occur at northern hemisphere sites, including Arctic fjords around Svalbard and the Porcupine Abyssal Plain. Molecular phylogenetic analyses, based on small subunit rRNA gene sequences, indicate that the new Weddell Sea species forms an independent lineage branching among monothalamous foraminiferans as a sister group to the clade of Psammophaga.

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A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts

Parasitology ◽

10.1017/s0031182000007149 ◽

2001 ◽

Vol 122 (1) ◽

pp. 93-103 ◽

Cited By ~ 240

Author(s):

M. CASIRAGHI ◽

T.J.C. ANDERSON ◽

C. BANDI ◽

C. BAZZOCCHI ◽

C. GENCHI

Keyword(s):

Sister Group ◽

Morphological Characters ◽

Coi Gene ◽

Phylogenetic Study ◽

Sister Group Relationship ◽

Rrna Gene ◽

Data Sets ◽

Gene Sequences ◽

Filarial Nematodes ◽

Molecular Phylogenetic

Infection with the endosymbiotic bacteria Wolbachia is widespread in filarial nematodes. Previous studies have suggested concordance between the phylogeny of Wolbachia with that of their nematode hosts. However, there is only one published molecular phylogenetic study of filarial species, based on the 5S rRNA gene spacer. The phylogeny proposed by this study is partially incongruent with previous classifications of filarial nematodes, based on morphological characters. Furthermore, both traditional classifications and molecular phylogenies are, in part, inconsistent with the phylogeny of Wolbachia. Here we report mitochondrial cytochrome oxidase I (COI) gene sequences for 11 species of filaria and for another spirurid nematode which was included as an outgroup. In addition, 16S rRNA, wsp and ftsZ gene sequences were generated for the Wolbachia of several filarial species, in order to complete the available data sets and further resolve the phylogeny of Wolbachia in nematodes. We used these data to evaluate whether nematode and Wolbachia phylogenies are concordant. Some of the possible phylogenetic reconstructions based on COI gene were congruent with the phylogeny of Wolbachia and supported the grouping of the rodent filaria Litomosoides sigmodontis with the lymphatic filariae (i.e. Brugia spp. and Wuchereria spp.) and the sister group relationship of Dirofilaria spp. and Onchocerca spp. However, the placement of the Wolbachia-free filaria Acanthocheilonema viteae is ambiguous and dependent on the phylogenetic methods used.

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Description and phylogenetic position of Corlissina maricaensis gen. nov., sp. nov. (Karyorelictea, Geleiidae), a novel interstitial ciliate from Brazil, with redefinition of the family Geleiidae

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.000579 ◽

2015 ◽

Vol 65 (Pt_12) ◽

pp. 4297-4308 ◽

Cited By ~ 4

Author(s):

Pedro H. Campello-Nunes ◽

Noemi Fernandes ◽

Martin Schlegel ◽

Inácio D. Silva-Neto

Keyword(s):

Novel Species ◽

Phylogenetic Analyses ◽

Cylindrical Body ◽

Sister Group ◽

18S Rrna Gene ◽

Phylogenetic Position ◽

Morphological Description ◽

Rrna Gene ◽

The Novel ◽

Cortical Granules

Corlissina maricaensis gen. nov., sp. nov. was obtained from samples of sediment collected in a brackish lagoon of Maricá city, Rio de Janeiro state, Brazil. The morphological description was based on live observations, after protargol staining, and scanning electron microscopy. The novel species has a cylindrical body shape that is slightly contractile, 230–550 × 35–65 μm, a cytoplasm with many globular inclusions, one row of irregular cortical granules between each somatic kinety, approximately 40–62 somatic kineties, two globular macronuclei measuring 9–24 μm and one micronucleus of approximately 4–9 μm. A subapical oral cavity was approximately 20–80 × 9–25 μm, with an adoral zone on the left side of the buccal field, which was composed of 32–60 polykineties and a paroral at the right side that was composed of 40–57 short polykineties. The new genus is distinguished from other geleiids by a loop-shaped posterior end of the paroral ciliature, made up of two rows of short polykineties, and the oralization of the central superior kinety (K0i), forming a row of dikinetids that borders the adoral zone internally, followed by several rows of monokinetids. In the phylogenetic analyses, the novel species was recovered as the sister group of Parduczia orbis with full support values based on 18S rRNA gene sequences. This work also indicates some problems in the definitions of the Geleiidae and proposes a new diagnosis for this karyorelictid family.

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Taxonomy of the Genus Halophila Thouars (Hydocharitaceae): A Review

Plants ◽

10.3390/plants9121732 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1732

Author(s):

John Kuo

Keyword(s):

Distinct Species ◽

Morphological Description ◽

Field Surveys ◽

West Pacific ◽

Taxonomic Description ◽

Morphological Studies ◽

Molecular Phylogenetic ◽

History Of ◽

West Indian Ocean ◽

Botanical Illustrations

The seagrass genus Halophila Thouars has more than twenty described species and is predominately distributed over a wide geographic range along the tropical and the warm temperate coastlines in the Indo-West Pacific Oceans. A brief history of the Halophila taxonomic development is presented. Based on reproductive and vegetative morphology, the genus is divided into eight sections including three new sections: section Australes, section Stipulaceae and section Decipientes. A rewritten taxonomic description of the type species for the genus Halophila,H. madagascariensis Steudel ex Doty et B.C. Stone, is provided. The lectotype of H. engelmannii Asch. as well as neotypes of H. hawaiiana Doty et B.C. Stone and H. spinulosa (Br.) Asch. are designated. Furthermore, H. ovalis ssp. bullosa, ssp. ramamurthiana and ssp. linearis together with H. balforurii have been recognised as distinct species. Nomenclature, typification, morphological description and botanical illustrations are presented for each taxon. Recent molecular phylogenetic surveys on certain Halophila taxa are also discussed. Field surveys for the deep water Halophila in West Pacific regions are suggested. Morphological studies combined with molecular investigations for the Halophila on the east coast of Africa and the West Indian Ocean are urgently needed and highly recommended.

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EVALUATION OF MOUNTAIN AREA AS NON-POINT SOURCE OF NITROGEN FOR SETO INLAND SEA: THE NORTHERN SHIKOKU REGION, JAPAN

Proceedings of International Conference "Managinag risks to coastal regions and communities in a changinag world" (EMECS'11 - SeaCoasts XXVI) ◽

10.31519/conferencearticle_5b1b9389d98135.84095825 ◽

2017 ◽

Author(s):

Shohei Morisawa ◽

Yukio Komai ◽

Takao Kunimatsu ◽

...

Keyword(s):

Average Concentration ◽

Point Sources ◽

Seto Inland Sea ◽

Mountain Streams ◽

Unit Load ◽

Mountain Areas ◽

Mountain Area ◽

The Seto Inland Sea ◽

Unit Load Method ◽

Important Position

The northern Shikoku region is located in the Western part of Japan and faces towards the Seto Inland Sea. The forest area, which is one of the non-point sources in the Seto Inland Sea watershed, occupies 75% of the land use in the watershed of the northern Shikoku region. The amount of loadings of nutrients and COD in the Seto Inland Sea has been estimated by the unit load method but actually the data has not been investigated. It is however, necessary to know the real concentration of nitrogen in mountain streams to evaluate the role which is the mountain area plays as non-point sources. Therefore, more water samples of mountain streams in the watershed need to be taken and the concentrations of nitrogen analyzed. The mountain streams in the northern Shikoku area were investigated from April, 2015 to November, 2015. The number of sampling sites was 283, in addition to the past data by Kunimatsu et al. The average concentration of nitrate nitrogen in Ehime, Kagawa, and Tokushima was 0.61mg/L, 0.78mg/L and 0.34mg/L, respectively. The environmental standard range for nitrogen in the Seto Inland Sea is from between less than 0.2mg/L and less than 1mg/L. Therefore, the average concentration of nitrogen in these regions was over category II, and those of mountain streams in Kagawa Prefecture exceeded category III. About 20% of mountain streams were more than 1mg/L. It has become clear that mountain areas occupy an important position as non-point sources for the Seto Inland Sea.

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