scholarly journals Growth Factors and Lymphokines: Modulators of Cholinergic Neuronal Activity

1991 ◽  
Vol 18 (S3) ◽  
pp. 390-393 ◽  
Author(s):  
Rémi Quirion ◽  
Dalia M. Araujo ◽  
Paul A. Lapchak ◽  
David Seto ◽  
Jean-Guy Chabot
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Acetylcholine Release ◽  
Interleukin 2 ◽  
Neuronal Population ◽  
Cholinergic Synapse ◽  
Positive Effects ◽  
Factor I ◽  
Epidermal Growth ◽  
Negative Factors

ABSTRACT:It is well known that various markers of the cholinergic synapse are altered in Alzheimer's Disease. Much interest is currently focussing on the evaluation of the possible efficacy of certain growth factors, especially nerve growth factor (NGF), to reduce or reverse cholinergic neuronal losses. Here we report that other growth factors (epidermal growth factor and insulin-like growth factor I) and a lymphokine, interleukin-2, are able to block acetylcholine release in the rat hippocampus. This suggests that while certain growth factors like NGF may have positive effects on the cholinergic neuron, others may act as “negative” factors on this neuronal population.

Stimulation of intestinal epithelial cell proliferation in culture by growth factors in human and ruminant mammary secretions

1987 ◽  
Vol 113 (2) ◽  
pp. 285-290 ◽  
Author(s):  
A. N. Corps ◽  
K. D. Brown
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Intestinal Epithelial ◽  
Epithelial Cell Proliferation ◽  
Factor I ◽  
Maximal Stimulation ◽  
Igf I ◽  
Epidermal Growth ◽  
Dose Dependent ◽  
Stimulation Of

ABSTRACT Samples of human and ruminant mammary secretions stimulated the proliferation of rat intestinal epithelial (RIE-1) cells in culture. The stimulation was dose-dependent, and samples taken prepartum had greater potency than those taken after parturition. When various hormones and growth factors known to be present in milk were tested, only epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) stimulated the proliferation of RIE-1 cells. IGF-I was effective at lower concentrations than insulin, and the maximal stimulation induced by each of these two polypeptides was greater than that induced by EGF. The maximal stimulation induced by samples of mammary secretions was similar to that induced by insulin or IGF-I. J. Endocr. (1987) 113, 285–290

Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture

1988 ◽  
Vol 116 (1) ◽  
pp. 97-100 ◽  
Author(s):  
D. Schams ◽  
R. Koll ◽  
C. H. Li
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Corpora Lutea ◽  
Dependent Manner ◽  
Insulin Like Growth Factor ◽  
Luteal Cells ◽  
Factor I ◽  
Igf I ◽  
Epidermal Growth ◽  
Growth Factor I

ABSTRACT The effect of insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), fibroblast growth factor (FGF) and nerve growth factor (NGF) on production of oxytocin and progesterone by cultured bovine granulosa and luteal cells was studied. Secretion of oxytocin was stimulated, in a dose-dependent manner, by IGF-I at 48 and 120 h of culture to levels much higher than those after stimulation with LH, FSH, EGF, FGF or NGF. A similar effect of IGF-I was observed for progesterone but, in contrast to oxytocin, secretion of progesterone was not increased by EGF, NGF or FGF. During primary culture, for 4 h, of dispersed bovine luteal cells obtained from corpora lutea between days 4 and 10 of the oestrous cycle, all the growth factors tested failed to stimulate secretion of oxytocin or progesterone. The data suggest the relevance of growth factors (especially IGF-I) for ovarian physiology and their possible importance for differentiation of follicles and luteinization. J. Endocr. (1988) 116, 97–100

scholarly journals c-ras-Ha gene expression is regulated by insulin or insulinlike growth factor and by epidermal growth factor in murine fibroblasts.

1989 ◽  
Vol 9 (8) ◽  
pp. 3411-3417 ◽  
Author(s):  
K H Lu ◽  
R A Levine ◽  
J Campisi
Keyword(s):  
Gene Expression ◽  
Growth Factors ◽  
Growth Factor ◽  
Quiescent Cells ◽  
Factor I ◽  
Insulinlike Growth Factor ◽  
Ha Gene ◽  
Murine Fibroblasts ◽  
Epidermal Growth ◽  
Insulinlike Growth Factor I

Although much is known about the structure of ras-encoded proteins, little is known about how expression is regulated. In serum-stimulated murine fibroblasts, c-ras-Ha mRNA levels fluctuated with the growth state but not with the position in the cell cycle. Two types of growth factors regulated c-ras-Ha expression: insulin (IN) or insulinlike growth factor I, each apparently acting through its cognate receptor, and epidermal growth factor (EGF). In quiescent cells, IN or insulinlike growth factor I induced c-ras-Ha mRNA three- to fivefold within 4 h, but thereafter the mRNA declined. By contrast, EGF had little effect in 4 h but induced the mRNA after 4 to 6 h. When quiescent cells were given serum or IN and EGF simultaneously, c-ras-Ha mRNA rose steadily, beginning 1 to 2 h after stimulation, and reached a stable five- to sevenfold elevation in 16 h. Thus, c-ras-Ha gene expression was sequentially regulated by two growth factors, one of which (IN) does not induce expression of other growth-regulated protooncogenes. A transformed derivative cell line that does not require IN for G1 progression has lost early IN-dependent but not late serum-dependent regulation. The results support the possibility that c-ras-Ha and IN action are functionally linked.

Characterization of insulin-like growth factor I and epidermal growth factor receptors in meningioma

1989 ◽  
Vol 71 (4) ◽  
pp. 538-544 ◽  
Author(s):  
Masaki Kurihara ◽  
Yoshiharu Tokunaga ◽  
Keisuke Tsutsumi ◽  
Tsutomu Kawaguchi ◽  
Kazuto Shigematsu ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Binding Sites ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
Number Of Binding Sites ◽  
Epidermal Growth ◽  
Growth Factor I

✓ Receptors for insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) were localized and characterized in eight samples of human meningioma (four fibrous, two meningothelial, and two angioblastic types), using quantitative autoradiographic techniques. Effects of both growth factors on deoxyribonucleic acid (DNA) synthesis in the cultured meningioma cells were examined. High numbers of specific binding sites for both IGF-I and EGF were homogeneously present in tissue sections derived from fibrous and meningothelial types of meningiomas, whereas binding sites for these growth factors were not detectable in adjacent leptomeninges. While relatively large numbers of IGF-I binding sites were located in the wall of the intratumoral vasculature, the number of binding sites in the stromal component was lower in angioblastic-type meningiomas, including a low number of EGF binding sites detected only in the stromal portion. Scatchard analysis revealed the presence of a single class of high-affinity binding sites for both IGF-I and EGF in the meningiomas examined (dissociation constant (Kd) = 0.6 to 2.9 nM, and the maximum number of binding sites (Bmax) = 16 to 80 fmol/mg for IGF-I; and Kd = 0.6 to 4.0 nM, Bmax = 3 to 39 fmol/mg for EGF). Both growth factors increased the synthesis of DNA, in a dose-dependent manner, as measured by 3H-thymidine incorporation. The combination of IGF-I and EGF synergistically stimulated the synthesis of DNA, and the effects seen with 10% fetal bovine serum could be reproduced at a concentration of 10−10 M. These observations can be interpreted to mean that both IGF-I and EGF may be involved in the growth modulation of meningiomas, possibly through paracrine or autocrine mechanisms.

c-ras-Ha gene expression is regulated by insulin or insulinlike growth factor and by epidermal growth factor in murine fibroblasts

1989 ◽  
Vol 9 (8) ◽  
pp. 3411-3417
Author(s):  
K H Lu ◽  
R A Levine ◽  
J Campisi
Keyword(s):  
Gene Expression ◽  
Growth Factors ◽  
Growth Factor ◽  
Quiescent Cells ◽  
Factor I ◽  
Insulinlike Growth Factor ◽  
Ha Gene ◽  
Murine Fibroblasts ◽  
Epidermal Growth ◽  
Insulinlike Growth Factor I

Although much is known about the structure of ras-encoded proteins, little is known about how expression is regulated. In serum-stimulated murine fibroblasts, c-ras-Ha mRNA levels fluctuated with the growth state but not with the position in the cell cycle. Two types of growth factors regulated c-ras-Ha expression: insulin (IN) or insulinlike growth factor I, each apparently acting through its cognate receptor, and epidermal growth factor (EGF). In quiescent cells, IN or insulinlike growth factor I induced c-ras-Ha mRNA three- to fivefold within 4 h, but thereafter the mRNA declined. By contrast, EGF had little effect in 4 h but induced the mRNA after 4 to 6 h. When quiescent cells were given serum or IN and EGF simultaneously, c-ras-Ha mRNA rose steadily, beginning 1 to 2 h after stimulation, and reached a stable five- to sevenfold elevation in 16 h. Thus, c-ras-Ha gene expression was sequentially regulated by two growth factors, one of which (IN) does not induce expression of other growth-regulated protooncogenes. A transformed derivative cell line that does not require IN for G1 progression has lost early IN-dependent but not late serum-dependent regulation. The results support the possibility that c-ras-Ha and IN action are functionally linked.

Insulin and epidermal growth factor are growth factors for isolated porcine thyroid follicles

1985 ◽  
Vol 110 (1_Suppla) ◽  
pp. S74
Author(s):  
R. GÄRTNER ◽  
W. GREIL ◽  
R. DEMHARTER ◽  
K. HORN
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epidermal Growth
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