On the Existence within the Liver Cells of Channels which can be directly injected from the Blood-Vessels

1904 ◽  
Vol 24 ◽  
pp. 65-69 ◽  
Author(s):  
E. A. Schäfer

In a series of papers dating from 1897, resumés of winch are given in the Bulletin international de I'académie des Sciences de Cracovie, Professor Browicz gives the results of observations on the liver cells, both in the normal state and in a pathologically altered condition, and draws from these observations certain important deductions. The most prominent fact which is recorded is the appearance (under normal conditions in the liver of the newborn child, and under pathological conditions in the adult human liver; under normal conditions, and after the intravenous injection of hæmoglobin some hours prior to death, in the liver of the dog) of erythrocytes, singly and in groups, and of free hæmoglobin and hæmoglobin crystals, as well as brown pigment, in the form both of granules and crystalline clumps, within the hepatic cells, both in the nucleus and in the cytoplasm. After affirming the undoubted existence of intracellular biliary passages communicating with intercellular bile ducts,—which intracellular passages he regards, not as accidental and temporary vacuoles, but as preformed channels within the cells, — Professor Browicz states that his observations, especially the presence within the liver cells of erythrocytes, demonstrate that the cells must be in communication with the hepatic capillaries.

The description by Schäfer (55) of a network of fine channels in the cells of the liver of the rabbit and cat which can be filled with injection material from the blood-vessels, and the confirmation of his observations in the livers of other animals as the result of our own experiments (26), have opened up several important questions concerning the minute anatomical structure of the liver. The presence of intracellular channels in the liver cells communicating with the blood-vessels is difficult to reconcile with the generally accepted views on the relations of the blood-vessels and lymphatics to the liver cells. Of late years several observers (Browicz (8), Schäfer (55)), have cast doubt on the presence of perivascular lymphatics in the liver lobules, and have suggested a direct supply of blood plasma from the vessels to the interior of the liver cells without interposition of lymph spaces. That the walls of the capillary blood-vessels of the liver possess a peculiar form of endothelial lining has been long recognised (Kupffer (37), Ranvier (50), and others). More recently Minot (45), from a study of the development of the liver vessels, has concluded that they are not true capillaries which have grown into the organ, but “sinusoids” which have been formed by a growth of the liver blastema into a large blood sinus, which, although having the appearance of capillaries, are actually spaces between the columns of liver cells lined by cells of an embryonic character. To resolve the question of the relationship of the blood lymph to the liver cells, we have in many kinds of animals injected the bile ducts in a number of animals and have further examined sections of liver with the same material. We have also injected the bile ducts in a number of animals and have further examined sections of liver stained by special methods. The results of our observations are recorded in this paper.


Author(s):  
Reza Afrisham ◽  
Sahar Sadegh-Nejadi ◽  
Reza Meshkani ◽  
Solaleh Emamgholipour ◽  
Molood Bagherieh ◽  
...  

Introduction: Obesity is a disorder with low-grade chronic inflammation that plays a key role in the hepatic inflammation and steatosis. Moreover, there are studies to support the role of exosomes in the cellular communications, the regulation of metabolic homeostasis and immunomodulatory activity. Accordingly, we aimed to evaluate the influence of plasma circulating exosomes derived from females with normal-weight and obesity on the secretion of inflammatory cytokines in human liver cells. Methods: Plasma circulating exosomes were isolated from four normal (N-Exo) and four obese (O-Exo) women. The exosomes were characterized and approved for CD63 expression (common exosomal protein marker) and morphology/size using the western blot and TEM methods, respectively. The exosomes were used for stimulation of HepG2 cells in vitro. After 24 h incubation, the protein levels of TNF-α,IL-6, and IL-1β were measured in the culture supernatant of HepG2 cells using the ELISA kit. Results: The protein levels of IL-6 and TNF-α in the cells treated with O-Exo and N-Exo reduced significantly in comparison with control group (P=0.039 and P<0.001 respectively), while significance differences were not found between normal and obese groups (P=0.808, and P=0.978 respectively). However, no significant differences were found between three groups in term of IL-1β levels (P=0.069). Based on the correlation analysis, the protein levels of IL-6 were positively correlated with TNF-α (r 0.978, P<0.001). Conclusion: These findings suggest that plasma circulating exosomes have probably anti-inflammatory properties independently from body mass index and may decrease the secretion of inflammatory cytokines in liver. However, further investigations in vitro and in vivo are needed to address the anti-inflammatory function of N-Exo and O-Exo in human liver cells and/or other cells.


1995 ◽  
Vol 28 (1) ◽  
pp. 118-128 ◽  
Author(s):  
MARQUÉ D. TODD ◽  
MICHAEL J. LEE ◽  
JULIE L. WILLIAMS ◽  
JOHN M. NALEZNY ◽  
PAULINE GEE ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yohei Tsukada ◽  
Fumitaka Muramatsu ◽  
Yumiko Hayashi ◽  
Chiaki Inagaki ◽  
Hang Su ◽  
...  

AbstractAngiogenesis contributes to numerous pathological conditions. Understanding the molecular mechanisms of angiogenesis will offer new therapeutic opportunities. Several experimental in vivo models that better represent the pathological conditions have been generated for this purpose in mice, but it is difficult to translate results from mouse to human blood vessels. To understand human vascular biology and translate findings into human research, we need human blood vessel models to replicate human vascular physiology. Here, we show that human tumor tissue transplantation into a cranial window enables engraftment of human blood vessels in mice. An in vivo imaging technique using two-photon microscopy allows continuous observation of human blood vessels until at least 49 days after tumor transplantation. These human blood vessels make connections with mouse blood vessels as shown by the finding that lectin injected into the mouse tail vein reaches the human blood vessels. Finally, this model revealed that formation and/or maintenance of human blood vessels depends on VEGFR2 signaling. This approach represents a useful tool to study molecular mechanisms of human blood vessel formation and to test effects of drugs that target human blood vessels in vivo to show proof of concept in a preclinical model.


2010 ◽  
Vol 30 (6) ◽  
pp. 566-573 ◽  
Author(s):  
Saura C. Sahu ◽  
Michael W. O'Donnell ◽  
Paddy L. Wiesenfeld

Metallomics ◽  
2017 ◽  
Vol 9 (3) ◽  
pp. 268-277 ◽  
Author(s):  
T. A. Marschall ◽  
N. Kroepfl ◽  
K. B. Jensen ◽  
J. Bornhorst ◽  
B. Meermann ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document