Differential Averaging of Single Molecule Images using Multivariate Statistical Classification

Author(s):  
Joachim Frank

It has been shown that averaging of single molecules or molecular assemblies visualized in the electron microscope is capable of giving reproducible results at the resolution level allowed by negative staining. The resolution achieved for a given specimen is, however, dependent upon the range of variation that the molecule exhibits in the electron microscope (fig. 1). A new technique, differential or local averaging, promises to overcome this limitation in fully utilizing the information contained in the experimental images.

1988 ◽  
Vol 7 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Liam T. Gallagher

Abstract. A new technique for viewing the same nannofossil specimen under the light microscope and scanning electron microscope is described, in which standard preparation materials are used. The applications of this, and previous techniques, are discussed as well as the relevance of these to the elucidation of taxonomic problems.


1990 ◽  
Vol 29 (Part 2, No. 4) ◽  
pp. L655-L658 ◽  
Author(s):  
Soh-ichiro Ozawa ◽  
Akira Yamanaka ◽  
Kunio Kobayashi ◽  
Yasumasa Tanishiro ◽  
Katsumichi Yagi

1980 ◽  
Vol 102 (1) ◽  
pp. 80-84 ◽  
Author(s):  
K. V. Kumar ◽  
M. Cozminca ◽  
Y. Tanaka ◽  
M. C. Shaw

A new technique for studying the performance of abrasive tools is described that is not only useful for evaluating the rate of wear of grinding systems but is also capable of yielding difficult to obtain data (such as the effective number of cutting points and local wheel work deflection) essential in fundamental studies of grinding. The new method involves overcut fly cutting with a small (1/8 in × 1/8 in) cluster of abrasive grains that are normally bound together and dressed. The small size of the specimen is particularly important relative to wear studies in that it enables wear data to be obtained with a relatively small amount of grinding and also permits the used abrasive to be studied in the scanning electron microscope.


2008 ◽  
Vol 103 (7) ◽  
pp. 07D931 ◽  
Author(s):  
P. Schattschneider ◽  
S. Rubino ◽  
M. Stoeger-Pollach ◽  
C. Hébert ◽  
J. Rusz ◽  
...  

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