Sites of Insulin Action Using Ferritin Labeling

1971 ◽  
Vol 29 ◽  
pp. 540-541
Author(s):  
Burton B. Silver ◽  
Ronald S. Nelson
Keyword(s):  
Electron Microscopy ◽  
Binding Sites ◽  
Anterior Pituitary ◽  
Insulin Action ◽  
Diabetic Rats ◽  
Insulin Antibody ◽  
Fat Pad ◽  
Target Organs ◽  
Uranium Salts ◽  
Sugar Levels

Some investigators feel that insulin does not enter cells but exerts its influence in some manner on the cell surface. Ferritin labeling of insulin and insulin antibody was used to determine if binding sites of insulin to specific target organs could be seen with electron microscopy.Alloxanized rats were considered diabetic if blood sugar levels were in excess of 300 mg %. Test reagents included ferritin, ferritin labeled insulin, and ferritin labeled insulin antibody. Target organs examined were were diaphragm, kidney, gastrocnemius, fat pad, liver and anterior pituitary. Reagents were administered through the left common carotid. Survival time was at least one hour in test animals. Tissue incubation studies were also done in normal as well as diabetic rats. Specimens were fixed in gluteraldehyde and osmium followed by staining with lead and uranium salts. Some tissues were not stained.

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

Potentiation of insulin action by a sulfonylurea in primary cultures of hepatocytes from normal and diabetic rats

Diabetes ◽  
1983 ◽  
Vol 32 (3) ◽  
pp. 206-212 ◽  
Author(s):  
A. I. Salhanick ◽  
P. Konowitz ◽  
J. M. Amatruda
Keyword(s):  
Insulin Action ◽  
Primary Cultures ◽  
Diabetic Rats

Specific Triiodothyronine Binding Sites in the Anterior Pituitary of the Rat

Science ◽  
1972 ◽  
Vol 176 (4040) ◽  
pp. 1252-1254 ◽  
Author(s):  
A. R. Schadlow ◽  
M. I. Surks ◽  
H. L. Schwartz ◽  
J. H. Oppenheimer
Keyword(s):  
Binding Sites ◽  
Anterior Pituitary

Effects of keishi-ka-jutsubu-to (traditional herbal medicine: Gui-zhi-jia-shu-fu-tang) on in vivo insulin action in streptozotocin-induced diabetic rats

Life Sciences ◽  
2003 ◽  
Vol 73 (21) ◽  
pp. 2687-2701 ◽  
Author(s):  
Bolin Qin ◽  
Masaru Nagasaki ◽  
Ming Ren ◽  
Gustavo Bajotto ◽  
Yoshiharu Oshida ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Insulin Action ◽  
Diabetic Rats ◽  
Traditional Herbal Medicine

In vitro insulin action on different ATPases of erythrocyte membranes in normal and diabetic rats

1985 ◽  
Vol 22 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Veena R. Agarwal ◽  
Anil K. Rastogi ◽  
Maharaj K. Sahib ◽  
Prem Sagar
Keyword(s):  
Insulin Action ◽  
Diabetic Rats ◽  
Erythrocyte Membranes

scholarly journals Terbufos-sulfone exacerbates cardiac lesions in diabetic rats: a sub-acute toxicity study

2016 ◽  
Vol 67 (2) ◽  
pp. 126-135 ◽  
Author(s):  
Syed M. Nurulain ◽  
Mohamed Shafiullah ◽  
Javed Yasin ◽  
Abdu Adem ◽  
Juma Al Kaabi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Organophosphorus Compounds ◽  
Lethal Dose ◽  
Density Lipoprotein ◽  
Cardiac Effect ◽  
Diabetic Rats ◽  
Ultrastructural Changes ◽  
Biochemical Tests ◽  
Wide Range ◽  
Cardiac Lesions

AbstractOrganophosphorus compounds (OPCs) have a wide range of applications, from agriculture to warfare. Exposure to these brings forward a varied kind of health issues globally. Terbufos is one of the leading OPCs used worldwide. The present study investigates the cardiac effect of no observable dose of a metabolite of terbufos, terbufos-sulfone (TS), under non-diabetic and streptozotocin-induced diabetic condition. One hundred nanomoles per rat (1/20 of LD50) was administered intraperitoneally to adult male Wister rats daily for fifteen days. The left ventricle was collected for ultrastructural changes by transmission electron microscopy. The blood samples were collected for biochemical tests including RBC acetylcholinesterase, creatinine kinase (CK), lactate dehydrogenase (LDH), cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides, ALT, AST, and GGT. The study revealed about 10 % inhibition of RBC-AChE in two weeks of TS treatment in non-diabetic rats whereas RBC-AChE activity was significantly decreased in diabetic TS treated rats. CK, LDH, and triglycerides were significantly higher in diabetic TS treated rats. Electron microscopy of the heart showed derangement and lesions of the mitochondria of cardiomyocytes in the TS treated groups. The present study concludes that a non-lethal dose of TS causes cardiac lesions which exacerbate under diabetic condition. Biochemical tests confirmed the ultrastructural changes. It is concluded that a non-lethal dose of TS may be a risk factor for a cardiovascular disease, which may be fatal under diabetic condition.

Effects of Quercetin and Coenzyme Q10 on Biochemical, Molecular, and Morphological Parameters of Skeletal Muscle in Trained Diabetic Rats

2021 ◽  
Vol 14 ◽  
Author(s):  
Amal M. Youssef ◽  
Dalia A. Mohamed ◽  
Samia Hussein ◽  
Doaa M. Abdullah ◽  
Shaimaa A. Abdelrahman
Keyword(s):  
Skeletal Muscle ◽  
Coenzyme Q10 ◽  
Combined Treatment ◽  
Diabetic Rats ◽  
Molecular Structures ◽  
Treadmill Training ◽  
Albino Rats ◽  
Musculoskeletal Function ◽  
Sugar Levels ◽  
Fibronectin Type Iii Domain

Background: Diabetes mellitus (DM) affects the musculoskeletal system through its metabolic perturbations. Exercise modulates blood sugar levels and increases the body’s sensitivity to insulin in patients with DM. Objective: This study aimed to investigate the potential effects of combined quercetin and coenzyme Q10 (CoQ10) supplements with or without exercise on the histological, biochemical and molecular structures of diabetic rat’s skeletal muscle. Method: A total of 64 adult male albino rats were divided into six groups: control, trained nondiabetic, non-trained diabetic, diabetic rats treated with combined CoQ10 and quercetin, diabetic rats with treadmill training, and diabetic rats treated with treadmill training and CoQ10 and quercetin. Blood and skeletal muscle samples were obtained from all groups for routine histological examination and biochemical determination of cytokine levels and protein activities. Quantitative real-time polymerase chain reaction (qRT-PCR) and morphometric analysis of PAS and Bax expressions were also performed. Results: Biochemical analysis revealed improvement in all studied parameters with combined CoQ10 and quercetin than exercise training alone. Combined treatment and exercise showed significant improvement in all parameters especially interleukin 6 and malondialdehyde. Fibronectin type III domain-containing protein 5 (FNDC5) expression and irisin levels increased in all trained groups but combined treatment with exercise significantly increased their levels than exercise alone. Histological analysis revealed improvement after exercise or combined treatment; however, when exercise was combined with CoQ10 and quercetin, marked improvement was observed. Conclusion: the combination of CoQ10 and quercetin could be promising in preserving musculoskeletal function in patients with DM concomitantly with physical exercise.

Sign in / Sign up

Export Citation Format

Share Document