Morphometric Analysis of Particulate Fractions from Rat Liver

1970 ◽  
Vol 28 ◽  
pp. 76-77
Author(s):  
Russel L. Deter
Keyword(s):  
Rat Liver ◽  
Morphometric Analysis ◽  
Quantitative Information ◽  
Small Samples ◽  
Morphological Evaluation ◽  
Cellular Autophagy ◽  
Quantitative Results ◽  
Lysosomal System ◽  
Morphologic Data

The morphological evaluation of fractions isolated by a variety of centrifugation methods has, until recently, been a qualitative one, based essentially on subjective impressions of small samples whose representativeness was not known. The lack of quantitative information about components of these fractions severely limited the usefulness of these morphologic data and made correlation with biochemical results rather difficult. In response to this situation, Baudhuin et al., and later Baudhuin and Berthet presented a method which circumvented previous problems and gave quantitative results directly comparable to those obtained by other methods. This method has been used in recent investigations concerned with the role of the lysosomal system in cellular autophagy carried out in this laboratory.

Inter-Tissue and Intra-Tissue Co-Expression Networks in Human Metabolism: Morphological Evaluation of the Link Between Transcription Factors ERβ and NFAT in Morbid Obesity

2020 ◽  
Vol 17 (1) ◽  
pp. 63-80
Author(s):  
Athina Chasapi ◽  
Kostas Balampanis ◽  
Eleni Kourea ◽  
Fotios Kalfaretzos ◽  
Vaia Lambadiari ◽  
...  
Keyword(s):  
Morbid Obesity ◽  
Adipose Tissue ◽  
Clinicopathological Parameters ◽  
Morbidly Obese ◽  
Human Metabolism ◽  
Activated T Cells ◽  
Alcoholic Fatty Liver ◽  
Morphological Evaluation ◽  
Underlying Mechanisms

Background: Estrogen receptor β (ERβ) plays an important role in human metabolism and some of its metabolic actions are mediated by a positive “cross-talk” with Nuclear Factor of Activated T cells (NFAT) and the key metabolic transcriptional coregulator Transcriptional Intermediary Factor 2 (TIF2). Introduction: Our study is an “in situ” morphological evaluation of the communication between ERβ, NFAT and TIF2 in morbid obesity. Potential correlations with clinicopathological parameters and with the presence of diabetes and non-alcoholic fatty liver disease (NAFLD) were also explored. The aim of the present study was to determine the role of ERβ and NFAT in the underlying pathophysiology of obesity and related comorbidities. We have investigated the expression of specific proteins using immunochemistry methodologies. Methods: Our population consists of 50 morbidly obese patients undergoing planned bariatric surgery, during which biopsies were taken from visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), skeletal muscle (SM), extramyocellular adipose tissue (EMAT) and liver and the differential protein expression was evaluated by immunohistochemistry. Results: We demonstrated an extensive intra- and inter-tissue co-expression network, which confirms the tissue-specific and integral role of each one of the investigated proteins in morbid obesity. Moreover, a beneficial role of ERβ and NFATc1 against NAFLD is implicated, whereas the distinct roles of TIF2 still remain an enigma. Conclusions: We believe that our findings will shed light on the complex underlying mechanisms and that the investigated biomarkers could represent future targets for the prevention and therapy of obesity and its comorbidities.

Role of nitric oxide in hepatic and pulmonary injury following ischemia-reperfusion of rat liver

2003 ◽  
Vol 124 (4) ◽  
pp. A719-A720
Author(s):  
Yuji Takamatsu ◽  
Kazuo Shimada ◽  
Koji Yamaguchi ◽  
Kazuo Chijiiwa ◽  
Masao Tanaka
Keyword(s):  
Nitric Oxide ◽  
Rat Liver ◽  
Ischemia Reperfusion ◽  
Pulmonary Injury

scholarly journals Role of hepatic lipase in the uptake and processing of chylomicron remnants in rat liver.

1994 ◽  
Vol 35 (4) ◽  
pp. 709-720
Author(s):  
S Shafi ◽  
S E Brady ◽  
A Bensadoun ◽  
R J Havel
Keyword(s):  
Rat Liver ◽  
Hepatic Lipase ◽  
Chylomicron Remnants

scholarly journals Power, the Pacific Islands, and the Prestige Press: A Case Study of How Climate Reporting is Influenced by UN Framework Convention on Climate Change Summits

2021 ◽  
pp. 194016122110180
Author(s):  
Meghan M. Shea ◽  
James Painter ◽  
Shannon Osaka
Keyword(s):  
Climate Change ◽  
Media Coverage ◽  
Pacific Islands ◽  
Structured Interviews ◽  
The Pacific ◽  
Emotional Appeals ◽  
Issue Attention ◽  
Quantitative Results

While studies have investigated UN Framework Convention on Climate Change (UNFCCC) meetings as drivers of climate change reporting as well as the geopolitical role of Pacific Islands in these international forums, little research examines the intersection: how media coverage of Pacific Islands and climate change (PICC) may be influenced by, or may influence, UNFCCC meetings. We analyze two decades of reporting on PICC in American, British, and Australian newspapers—looking at both volume and content of coverage—and expand the quantitative results with semi-structured interviews with journalists and Pacific stakeholders. Issue attention on PICC increases and the content changes significantly in the periods around UNFCCC meetings, with shifts from language about vulnerability outside of UNFCCC periods to language about agency and solutions. We explore the implications of these differences in coverage for both agenda setting and the amplification of emotional appeals in UNFCCC contexts.

scholarly journals Role of calcium as an inhibitor of rat liver carbamylphosphate synthetase I.

1984 ◽  
Vol 259 (1) ◽  
pp. 323-331 ◽  
Author(s):  
S Cerdan ◽  
C J Lusty ◽  
K N Davis ◽  
J A Jacobsohn ◽  
J R Williamson
Keyword(s):  
Rat Liver ◽  
Carbamylphosphate Synthetase
Sign in / Sign up

Export Citation Format

Share Document