Electron microscopic study on the phagocytic lining cells in the cavernous body of the lamprey gill

1978 ◽  
Vol 36 (2) ◽  
pp. 448-449
Author(s):  
Kazuhito Yamaguchi ◽  
Kazuhiko Awaya
Keyword(s):  
Electron Microscope ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Embedded Blocks ◽  
Ultrathin Sections ◽  
Branchial Artery ◽  
Scanning Electron ◽  
Cavernous Body

The lining cells in the cavernous body, which is a branch of the afferent branchial artery, of lamprey gills were studied with transmission (TEM) and scanning electron microscope (SEM).Adult and larval lampreys, Lampertra planeri, were used in this study. Lamprey gills removed after perfusion were fixed in 2 % glutaraldehyde/formaldehyde (pH 7. 4) for 1 hour and postfixed in 1 % osmic acid. For TEM, the gills were dehydrated in ethanol and embedded in epon 812. Ultrathin sections were doubly stained with uranyl acetate and lead citrate. For SEM, most of the gills were dehydrated in ethanol and isoamylacetate and cracked in liquid nitrogen. Some of the gills dehydrated were embedded in stylene monomer. Stylene embedded blocks were cracked with a hummer and dissolved in propylene oxide. All the specimens were dried in a critical point dryer and coated 100 Å thickness with gold-paladium.The cavernous body is composed of trabeculae, between which are blood spaces, the lacunae.

Ultrastructural characteristics on capillary of giant panda

1990 ◽  
Vol 48 (3) ◽  
pp. 491-491
Author(s):  
Zeng Xiang-Yuan ◽  
Chong Hang ◽  
Lian Wei ◽  
Zhang Ke-juing
Keyword(s):  
Microscopic Observation ◽  
Giant Panda ◽  
Electron Microscopic Observation ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Lead Citrate ◽  
Capillary Diameter ◽  
Electron Microscopic ◽  
Ultrathin Sections ◽  
Ultrastructural Characteristics

Electron microscopic observation on ultrastructural characteristics of the capillary ofgiant panda was presented. The samples were taken from mesentery and another samples were taken from mucosa of intestine. They were fixed in 2.5% phosphatebuffered glutaraldehyde and 1% osmic acid, and dehydrated by graded series of acetone. Then they were embedded with Epon 812. For orientation under phasecontrast microscope, 0.5--1.0 u thick sections were prepared from the epoxyembedded material. After that, ultrathin sections (30-50) were taken with ultramicrotome LKB-V type and stained by uranyl acetate and lead citrate and examined under DXB2-12 Electron Microscope.Electron microscopic observation showed the capillary diameter in the mesentery microcirculation is larger and capillary wall is thin. The nucleus in endothelium was large and wasn't regular in the shape. Chromatin was loose in the nucleus. There was little plasma inthe endothelium and it looked loose. Organelles in the cytoplasm were little and vacuoles were more in endothelium. The distribution of plasma in the endothelium was different with endothelium of human body.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Human Stapes Crura: Normal Ultrastructure. Scanning Electron Microscopic Findings

1975 ◽  
Vol 33 ◽  
pp. 528-529
Author(s):  
M.D. Graham
Keyword(s):  
Electron Microscope ◽  
Surgical Treatment ◽  
Scanning Electron Microscope ◽  
Osmic Acid ◽  
Human Cadaver ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Normal Human ◽  
Microscopic Findings ◽  
Scanning Electron

The recent development of the scanning electron microscope has added great impetus to the study of ultrastructural details of normal human ossicles. A thorough description of the ultrastructure of the human ossicles is required in order to determine changes associated with disease processes following medical or surgical treatment.Human stapes crura were obtained at the time of surgery for clinical otosclerosis and from human cadaver material. The specimens to be examined by the scanning electron microscope were fixed immediately in the operating room in a cold phosphate buffered 2% gluteraldehyde solution, washed with Ringers, post fixed in cold 1% osmic acid and dehydrated in graded alcohol. Specimens were transferred from alcohol to a series of increasing concentrations of ethyl alcohol and amyl acetate. The tissue was then critical point dried, secured to aluminum stubs and coated with gold, approximately 150A thick on a rotating stage in a vacuum evaporator. The specimens were then studied with the Kent-Cambridge S4-10 Scanning Electron Microscope at an accelerating voltage of 20KV.

The fine structure of ascus development in Lasiobolus monascus (Pezizales)

1978 ◽  
Vol 56 (7) ◽  
pp. 862-872 ◽  
Author(s):  
James W. Kimbrough ◽  
Gerald L. Benny
Keyword(s):  
Fine Structure ◽  
Uranyl Acetate ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Stalk Cell ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Ultrathin Sections ◽  
Microscopic Inspection ◽  
Physical And Chemical

Ultrastructural and cytochemical studies on the ascus of Lasiobolus monascus are presented. Apothecia in various stages of development were obtained in culture and prepared for both light and electron microscopic observations. Ultrathin sections for electron microscopic inspection were often treated with silver methenamine to enhance wall characteristics. Ascus development was followed from fertilization to maturity.In this species, the ascogonium enlarges after fertilization to become the ascus mother cell. Two pores are present in the young ascus, one connecting it to the antheridium and another between the ascus and stalk cell. The ultrastructural features of these pores in the young and maturing ascus are described. During ascus enlargement, as many as four wall layers are found when poststained with silver methenamine. Only two layers are clearly distinguishable when poststained with uranyl acetate and lead citrate. The apical zone of dehiscence is characterized by a distinct annular swelling which appears during early ascosporogenesis. By spore maturation, this swelling is not evident either at the light or electron microscopic level. Instead, there appear to be both physical and chemical changes in the area of dehiscence. The wall is distinctly thinner and much more electron transparent in the area of dehiscence when treated with silver methanamine.

Electron Microscopic Study of a Spontaneous Rat Tumor

1971 ◽  
Vol 29 ◽  
pp. 322-323
Author(s):  
P. W. Cole ◽  
R. M. Jamison
Keyword(s):  
Propylene Oxide ◽  
Uranyl Acetate ◽  
Female Rat ◽  
Lead Citrate ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Non Invasive ◽  
Ultrathin Sections ◽  
Glass Knife ◽  
Rat Tumor

A spontaneously occurring, non-invasive mammary tumor was observed in a 7-month-old, non-lactating, random-bred Sprague-Dawley female rat. The tumor was located subcutaneously in the distal mammary line as a firmly encapsulated, lobated growth. The tumor was surgically removed and immersed in Clark's buffer containing 4% glutaraldehyde. (Later conventional histochemical studies revealed this tumor to be a relatively non-differentiated fibro-adenoma.) Exterior and interior portions of the tumor were excised and fixed separately. The tissues were cut into 1 mm cubes and fixed for 4 hours in 4% glutaraldehyde. The specimens were then washed 4 times in buffer and left overnight at 4°C in the buffer. The cubes were postfixed in 2% OsO4 for 2 hours, after which they were dehydrated in a graded series of ethanol. The specimens were then washed with 2 changes of propylene oxide and perfused with a 1:1 mixture of propylene oxide-Epon 812 for 1 hour. Next, the tissue cubes were embedded in a mixture of Epon 812, DDSA, NMA, and DMP 30. Polymerization was allowed to proceed sequentially at 37°C overnight, 45°C for 8 hours, and 60°C for 24 hours. Ultrathin sections were cut with the Porter-Blum MT-2B ultramicrotome equipped with a glass knife. Sections were picked up on copper grids and stained with saturated uranyl acetate and 0.2% lead citrate. Specimens were examined in the Philips 300 electron microscope at instrumental magnifications ranging from 3,000-20,000 times.

Electron Microscopic Studies of Gerbil Testis After Vasectomy

1976 ◽  
Vol 34 ◽  
pp. 154-155
Author(s):  
S. K. MAJUMDAR ◽  
FRED KALENSCHER
Keyword(s):  
Electron Microscope ◽  
Normal Structure ◽  
Uranyl Acetate ◽  
Meriones Unguiculatus ◽  
Mongolian Gerbils ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Acetate Lead ◽  
Microscopic Studies ◽  
Ultrathin Sections

Ultrathin sections made from bilaterally vasectomized as well as bilaterally sham-operated Mongolian gerbils (Meriones unguiculatus) were examined and compared under an electron microscope in order to determine whether vasectomy has any effect upon the fine structure of the testis. The whole testes were removed and placed in Karnovsky's fixative for one hour. After this period the testes were diced into small pieces and fixed for an additional hour in the same fixative. After rinsing in distilled water and postfixed for one hour in OsO4, the tissues were embedded in Epon 812. The sections were stained with uranyl acetate-lead citrate and examined on a Philips Model 201 transmission electron microscope. Shamoperated testis exhibited normal structure of germ cells.

Ultrastructure of Angiosarcoma of Mouse Tail

1980 ◽  
Vol 38 ◽  
pp. 740-741
Author(s):  
White Yvonne ◽  
Winslow Sheldon ◽  
James W. Townsend ◽  
Neil A. Littlefield
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Toluidine Blue ◽  
Female Mouse ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Ultrathin Sections ◽  
Resin Mixture

Spontaneous neoplasms rarely occur on the tails of BALB/cStCrlfC3H/Nctr mice, but the neoplasm most frequently observed is a locally invasive non-metastatic angiosarcoma. In this case a female mouse weighing 33.2 g, 706 days of age, presented a soft, red, irregularly-shaped mass, measuring 18 mm in its greatest dimension, in the subcutis of the base of the tail. A portion of the tail tumor was taken for electron microscopy and the remainder was processed for light microscopy. The tissue processed for electron microscopy was fixed in 4% cacodyl ate-buffered glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated in a graded series of ethanol solutions, and embedded in Epon-Araldite resin mixture. Sections of 1 μm were stained with toluidine blue for light microscopy and ultrathin sections of 100 nm were stained with uranyl acetate and lead citrate, then examined with a Philips EM201 electron microscope .

X-ray microanalytical and enzyme-cytochemical study on vesical malacoplakia

1978 ◽  
Vol 36 (2) ◽  
pp. 646-647
Author(s):  
Masami Hokano ◽  
Tsunao Oh-I ◽  
Yoshie Narita ◽  
Hiroshi Sassa ◽  
Saburo Suzuki
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Granulomatous Inflammation ◽  
Uranyl Acetate ◽  
The Other ◽  
Lead Citrate ◽  
Cytochemical Study ◽  
X Ray ◽  
Ultrathin Sections

Malacoplakia is a kind of granulomatous inflammation and characterized by the presence of calcium-stain positive granules (Michaelis-Gutmann bodies, hereinafter abbreviated as M-G bodies ) in the macrophages.In this report we want to say about the following articles:the ultrastruc tural findings in four cases of vesical malacoplakia;the ultrastructural morphogenesis of the M-G bodies;X-ray microanalytical studies which examine the change of the chemical component of M-G bodies, according to their developing stages; andacid phosphatase activity of lysosome within the malacoplakic macrophages.Biopsy materials taken from four cases with vesical malacoplakia were divided into 2 parts; one for a light microscopy and the other for an electron microscopy. The specimen for an electron microscopy was fixed in glutaraldehyde and osmium tetroxide, dehydrated in ethanol and then embedded in Epon 812. Ultrathin sections were doublestained with lead citrate and uranyl acetate and then subjected to routin TEM observation. For X-ray microanalysis was mounted an energy dispersive X-ray microanalyzer on a JEM-100 C type electron microscope. Ultrathin sections for microanalysis were cut 100-200nm thich and not stained.

Ultrastructure of Developing Granulosa and Theca Cells

1970 ◽  
Vol 28 ◽  
pp. 92-93
Author(s):  
Iracema M. Baccarini
Keyword(s):  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Ether Anesthesia ◽  
Rat Fetuses ◽  
Theca Cells ◽  
The Past ◽  
Microscopic Studies

The embryology of granulosa and theca cells is not understood thoroughly. Electron microscopic studies in the past have been concerned mainly with mature granulosa cells and less with their development.Material and Methods. Rat fetuses were removed surgically under ether anesthesia at 16-17, 17-18 and 18-19 days of gestation. Their abdominal cavities were opened, and the fetuses were placed immediately into 3% glutaraldehyde (pH 7.2) for 3 hours. During this time, the fetal ovaries were dissected under a microscope. The tissue was washed in phosphatebuffer for 24 hours, post-fixed in 1% phosphate buffered osmium tetroxide for 1-2 hours at 4°C, and embedded in Durcupan ACM (Fluka). Sections were double stained with uranyl acetate and lead citrate, and viewed in an RCA-EMU-3D electron microscope.

Ultrastructure of the Ascospores and Conidia of Venturia Inaequalis

1973 ◽  
Vol 31 ◽  
pp. 466-467
Author(s):  
A. L. Granett
Keyword(s):  
Osmium Tetroxide ◽  
Venturia Inaequalis ◽  
Uranyl Acetate ◽  
Distilled Water ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Apple Leaves ◽  
Ultrathin Sections ◽  
Ultrastructural Characteristics ◽  
Microscopic Investigations

Venturia inaequalis (Cke.) Wint. is the causal agent of scab,a worldwide disease of apples. There have been few electron microscopic investigations on any phase of this fungus. The studies reported herein were initiated to clarify some ultrastructural characteristics of ascospores and conidia.In the spring ascospores develop within perithecia on decaying apple leaves from the previous year. Either whole perithecia were teased from leaves, or ascospores were isolated from leaves in an air tunnel device. Conidia develop on living leaves, blossoms, and fruits. Infected leaves were sprayed with distilled water and these spores were collected and concentrated into a pellet by low speed centrifugation. Perithecia, ascospores, and conidia were fixed in glutaraldehyde and osmium tetroxide; ultrathin sections were stained with uranyl acetate and lead citrate.

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