Gap junction associated filaments in testis interstitial cells of the rat
The testis interstitial cells of the rat are closely associated forming small aggregates in the vicinity of the vessels. The finality of the present study is to analise the relationship between the interstitial cells and to describe some particularities of their junctions.The observations were made in thin sections from testis of 2 to 3 months old rats fixed by immersion or perfusion with 2 to 3% glutaral- dehyde in s-collidine or cacodylate buffer followed by 2% osmium tetro-xyde in the same buffers. Some specimens have been prepared following the technique of Shea. The thin sections obtained after embedding in Epon were double stained in uranyl acetate and lead citrate. For the cryofracture study the tissues were fixed in 3% glutaraldehyde in cacodylate buffer, impregnated in 28% glycerol, frozen in Freon 22 and stored in liquid nitrogen. Freeze fracture and platinum-carbon shadowing were done in a Balzers 300.