Ultrastructural Changes in the Brain Following Local Application of Guinea Pig Serum

1972 ◽  
Vol 30 ◽  
pp. 342-343
Author(s):  
Barbara G. Bystrom ◽  
Anatol Costin ◽  
W. Ross Adey
Keyword(s):  
Guinea Pig ◽  
Physiological Saline ◽  
Lateral Geniculate Body ◽  
Microscopy Study ◽  
Uranyl Acetate ◽  
Electron Microscopy Study ◽  
Ultrastructural Changes ◽  
Lateral Geniculate ◽  
The Brain ◽  
Pig Serum

It was previously reported that evoked potentials and impedance were diminished when guinea pig serum was introduced into the lateral geniculate body of the cat. The cats used in the present study were chronically implanted with electrodes and cannulae. Two hours after injection of 1 μl guinea pig serum directly into the lateral geniculate body, when electrophysiological changes were evident, the cats were sacrificed. The brains were perfused with phosphate buffered 2%Glutaraldehyde-2% formaldehyde after flushing with physiological saline. Post fixation was with 1% OsO4. Dehydration was carried out in graded Ethanol, and embedment was in Epon. The silver to silver-grey sections were intensified with uranyl acetate and lead citrate. In all cases, one lateral geniculate body was used for control and the other for experimental injection.The electron microscopy study was done "blind" in that the treatment, if any, or the site of injection, if any, was unknown by the microscopist.

Iron localization in the guinea pig choroid plexus: A light and transmission electron microscopy study

2018 ◽  
Vol 49 ◽  
pp. 128-133 ◽  
Author(s):  
Marilda da Cruz Fernandes ◽  
Antônio Generoso Severino ◽  
Adriana Maria Zago ◽  
Lucas Tortorelli ◽  
Fabiano B. Carvalho ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Guinea Pig ◽  
Choroid Plexus ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Transmission Electron Microscopy Study ◽  
Transmission Electron

A Transmission Electron Microscopy Study and Viability Evaluation of the Effect of 2450 MHz Microwaves on Candida albicans.

1997 ◽  
Vol 3 (S2) ◽  
pp. 117-118
Author(s):  
C. Melgoza ◽  
J. Sepúlveda ◽  
M. Moreno ◽  
M. A. Rodríguez
Keyword(s):  
Microscopy Study ◽  
Uranyl Acetate ◽  
Electron Microscopy Study ◽  
Microwave Oven ◽  
Yeast Cells ◽  
Growth Media ◽  
Mitochondrial Matrix ◽  
Transmission Electron Microscopy Study ◽  
Thin Sections ◽  
Transmission Electron

Microwaves of 2450 MHz have been used as a heating procedure in food technology, tissue processing for histological studies (1), in medicine (2) and as a sterilization agent for medical devices (3). Experiments were carried out exposing yeast cells of Candida albicans suspended in growth media to different times of radiation at 750 watts in a commercial microwave oven. Batches of 5 tubes containing the cells suspension were radiated from 15 seconds to 3 minutes and colony counts were done after exposure. Viability ceased totally after one minute radiation. Samples were obtained from unexposed controls and tubes radiated for 15, 30, 45 and 60 seconds, centrifuged and the pellets fixed in 1.5% potasium permanganate. After embbeding in Epon, thin sections were obtained and stained with uranyl acetate. Micrographs were taken with a Zeiss EM-109 electron microscope. Cells showed alteration of internal membranes at early times, and later changes indicative of cytoplasmic coagulation and organelle disorganization were seen. At 15 seconds mitochondrial matrix showed alterations followed by damage of its membranes.

scholarly journals REGRESSION OF TRANSPLANTED LYMPHOMAS INDUCED IN VIVO BY MEANS OF NORMAL GUINEA PIG SERUM

1953 ◽  
Vol 98 (6) ◽  
pp. 583-606 ◽  
Author(s):  
John G. Kidd
Keyword(s):  
Guinea Pig ◽  
Active Material ◽  
Experimental Studies ◽  
Physiological Saline ◽  
Normal Tissues ◽  
Physiological Saline Solution ◽  
Microscopic Studies ◽  
Pig Serum

In an extension of the experimental studies recorded in an associated paper; attempts were made to isolate and characterize the constituent of guinea pig serum responsible for inducing regression of transplanted lymphomas in vivo. The active material was precipitated readily from the whole serum, along with some of the globulins, by means of ammonium sulfate in concentrations of 2.0 molar or greater; it withstood heating at 56°C. for 20 or 30 minutes, but was inactivated upon heating at 66°C. for similar periods; it was completely inactivated by chymotrypsin in concentrations of 1 or 2 mg./cc. during 6 hours at 37°C. Furthermore, the inhibitory effects of small amounts of the guinea pig serum in vivo were enhanced upon admixture with immune sera prepared by injecting the lymphosarcoma cells into rabbits. The facts as a whole suggest that the active material is a protein, and that it may be one or another of the components of complement; yet they do not suffice to establish its identity. Microscopic studies showed that the cells of subcutaneous lymphomas rapidly died and were resorbed following injections of relatively large amounts of guinea pig serum intraperitoneally into mice carrying them, while similar changes followed more gradually after repeated injections of smaller amounts of guinea pig serum. No changes referable to the guinea pig serum were seen in the normal tissues or organs of mice receiving it. Mouse lymphoma cells, suspended artificially as individuals in a physiological saline solution, regularly remained viable following incubation in vitro in mixture with guinea pig serum during 6 hours at 37°C. The finding provides strong evidence that the regression of lymphomas that follows injection of guinea pig serum in vivo is brought about through some reaction in which the guinea pig serum and the host both participate. Some of the implications of the findings are discussed.

scholarly journals Histochemical assessment of Moringa – oleifera oil and walnut oil on cadmium induced lateral geniculate body damage in developing male Wistar rats (Rattus novergiccus)

2019 ◽  
Vol 8 (2) ◽  
pp. 1593-1605
Author(s):  
O.D. Omotoso ◽  
S.A. Adelakun ◽  
B.P. Akwu ◽  
E. Ogbonna ◽  
I.J. Idomeh
Keyword(s):  
Vitamin C ◽  
Oxidative Damage ◽  
Moringa Oleifera ◽  
Lateral Geniculate Body ◽  
Walnut Oil ◽  
Lateral Geniculate ◽  
Male Wistar Rats ◽  
Group A ◽  
Moringa Oleifera Oil ◽  
The Brain

The brain is vulnerable to oxidative damage due to its high oxygen consumption. This Study investigate the effects of cadmium on the lateral geniculate body of developing male wistar rats and ameliorative potential of Moringa oleifera seed oil and walnut oil extracts. Seven groups of five animals each were used in this experiment. Group A received 3ml of 0.9% normal saline; group B received 2.5mg/kg bw of 3CdSO4.8H2O, group C received 5mg/kg bw vitamin C & 6mg/kg bw vitamin E, group D received 5mg/kg bw vitamin C & 6mg/kg bw vitamin E + 2.5mg/kg bw Cd, group E received 2.5mg/kg bw Cd + 4mg/kg bw Moringa oleifera oil, group F received 2.5mg/kg bw Cd + 4mg/kg bw walnut oil, while group G received 2.5mg/kg bw Cd + 2mg/kg bw walnut + 2mg/kg bw Moringa oleifera oil concomitantly for 3weeks. Parameter tested includes LDH, G6PD in brain tissues, SOD and GPx enzymes in brain homogenates and serum and cresyl fast violet stain in the brain tissues. Cd administration significantly increased SOD, GPx, LDH and decreased G6PD level in brain tissue and decreased their activity in serum when compared with Group A control rats. There was marked reduction and lost in the distribution of nissl substances of the studied tissues of Cd administered animals. However, administration of vitamin C & E, walnut and Moringa oleifera oil restored damaged tissues. Walnut and Moringa oleifera seed oil therefore attenuated the oxidative damage and morphological changes induced by cadmium in the lateral geniculate body of the brain of the young male wistar rats.Key Words: Lateral geniculate body, Antioxidant, Histochemical, Cadmium, Oxidative Damage

scholarly journals Antiviral Activity of β-l-2′,3′-Dideoxy-2′,3′-Didehydro-5-Fluorocytidine in Woodchucks Chronically Infected with Woodchuck Hepatitis Virus

2001 ◽  
Vol 45 (4) ◽  
pp. 1065-1077 ◽  
Author(s):  
F. Le Guerhier ◽  
C. Pichoud ◽  
C. Jamard ◽  
S. Guerret ◽  
M. Chevallier ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Hepatitis Virus ◽  
Acute Infection ◽  
Skin Pigmentation ◽  
Antiviral Effect ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Ultrastructural Changes ◽  
Woodchuck Hepatitis Virus ◽  
Viral Spread

ABSTRACT The l-nucleoside analog β-l-2′,3′-dideoxy-2′,3′-didehydro-5-fluorocytidine (β-l-Fd4C) was first shown to exhibit potent activity against hepatitis B virus (HBV) in tissue culture and then to significantly inhibit viral spread during acute infection in the duck HBV model (F. Le Guerhier et al., Antimicrob. Agents Chemother. 44:111–122, 2000). We have therefore examined its antiviral activity in a mammalian model of chronic HBV infection, the woodchuck chronically infected with woodchuck hepatitis virus (WHV). Side-by-side comparison of β-l-Fd4C and lamivudine administered intraperitoneally during short-term and long-term protocols demonstrated a more profound inhibition of viremia in β-l-Fd4C-treated groups. Moreover, β-l-Fd4C induced a marked inhibition of intrahepatic viral DNA synthesis compared with that induced by lamivudine. Nevertheless, covalently closed circular (CCC) DNA persistence explained the lack of clearance of infected hepatocytes expressing viral antigens and the relapse of WHV replication after drug withdrawal. Liver histology showed a decrease in the inflammatory activity of chronic hepatitis in woodchucks receiving β-l-Fd4C. An electron microscopy study showed the absence of ultrastructural changes of hepatic mitochondria, biliary canaliculi, and bile ducts. However, a loss of weight was observed in all animals, whatever the treatment, as was a transient skin pigmentation in all woodchucks during β-l-Fd4C treatment. There was no evidence that lamivudine or β-l-Fd4C could prevent the development of hepatocellular carcinoma with the protocols used. These results indicate that β-l-Fd4C exhibits a more potent antiviral effect than lamivudine in the WHV model but was not able to eradicate CCC DNA and infected cells from the liver at the dosage and with the protocol used.

Unusual Inclusions in Neutrophils of two Human Renal Allografts

1976 ◽  
Vol 34 ◽  
pp. 242-243
Author(s):  
R. Valenzuela ◽  
S.D. Deodhar ◽  
D.G. Osborne ◽  
W.E. Braun ◽  
L.H.W. Banowsky
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Renal Allograft ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Uranyl Acetate ◽  
Electron Microscopy Study ◽  
Thin Sections ◽  
Blood Disorder ◽  
Cadaver Donor

During a retrospective light and electron microscopy study of 158 human renal allograft biopsies, we observed, in two cases, unusual inclusions in the neutrophils trapped in glomeruli and paratubular capillaries. For electron microscopy, the specimens were fixed in glutaraldehyde, post-fixed in osmium tetroxide and embedded in Epon. Thin sections were stained with uranyl acetate and lead citrate. Eighty-six of the electron microscopically examined biopsies did not contain any neutrophils for evaluation, and only 10 biopsies showed four or more neutrophils.The two patients, A age 35 and B age 42, had received a cadaver donor renal allograft because of chronic renal failure secondary to hereditary nephritis and nephrosclerosis respectively. Neither patient was affected by any known primary blood disorder.

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