Low-cost digital-image processing and analysis system for on-line morphometric measurements of sem images

Author(s):  
Michael E. Rosenfeld

The widespread use of digital image processing systems for high resolution morphometric applications has been limited by the cost and difficulty of interfacing systems to electron microscopes. The recent development of image acquisition boards and software for PC-AT based systems and the availability of scanning electron microscopes that are capable of scanning at true TV rates (RS-170 compatible signals), have alleviated these problems. We have assembled a system consisting of a Compaq Portable 286 (Compaq Computer Corp. Houston, TX) equipped with an FG-100 image acquisition board (Imaging Technology Inc. Woburn, MA), interfaced with a Philips 515 SEM containing a motorized stage and Edax stage controller (Philips Electronics Inc. Mahwah, NJ).Utilizing commercially available software, this system has extensive image processing and morphometric analysis capabilities. For example, because TV scan rates generate images with low signal to noise ratios, improvement of the image quality is possible via real time image averaging and background subtraction using the the FG-100 board Feedback/Input lookup table.

2021 ◽  
Vol 31 (2) ◽  
pp. 15-21
Author(s):  
A. A. Makarenko

Problem statement. Search of plants on the observable image is important function of the UAV onboard optoelectronic systems. One of methods of the observable surface image analysis is the textural analysis which allows to select homogeneous areas on the observable image and to define contours of the plants which are covered with various structures and not having boundaries accurately defined on the image.The purpose. To develop algorithm of the textural analysis of a site terrestrial or a water surface, fulfilled by the digital image processing. In paper the image textural analysis algorithm fulfilled on the basis of an evaluation of local spectral and statistical performances of the image is presented.Results. The algorithm according to which for each element of the analyzed image in a square environ from 1024 elements the local spectra oriented under different angles and local variances are calculated is developed. Calculated by digital image processing amplitudes of a spectrum and variances values characterize texture parameters in a environ of each element of the image. Points of considerable modifications of local properties define position of boundaries between various structures.The practical importance. Results of the considered algorithm trials as a part of simulation model have shown a possibility of its application in the UAV onboard image observed analysis system.


2013 ◽  
Vol 706-708 ◽  
pp. 765-770
Author(s):  
Ming Zhu Lu

In this paper, after the simple and practical hardware is connected with the host, the software system is used to count the colony of bacteria, the results are statistically analyzed, and the obtained data can be displayed, saved and printed. The software is developed with VC++, using digital image processing knowledge to do some research on automatic analyzing the count of colony.


2021 ◽  
Vol 125 ◽  
pp. 181-192
Author(s):  
Igor R. Fermo ◽  
Thiago S. Cavali ◽  
Lucas Bonfim-Rocha ◽  
Caio L. Srutkoske ◽  
Franklin C. Flores ◽  
...  

2020 ◽  
Author(s):  
José Magalhães ◽  
Gessica Pereira ◽  
Alexsander Leão ◽  
Carolina Scherer

<p>This study reveals the use of high resolution images collected by small Unmanned Aerial Vehicle (UAV) and Digital Image Processing (DIP) from Structure from Motion (SfM) technique applied to the prospection and geometric characterization of fossil tanks in the Guanambi region, located at Bahia state, Brazil. Geologically, the region is located in the Guanambi Batolith, composed of granites, migmatites and orthognaisses. In the research region for example, there was the Lagoa das Abelhas fossiliferous tank, which was previously excavated and in which bone fragments of various pleistocene mammal taxa, such as those of the order Xenarthra, were found, represented by sloths, glyptodonts and armadillos. Considering that there are no records of an effective scientific method to identify these features, the main objective of this work is to map the distribution of fossiliferous tanks excavated as well as those with prospective potential, and to estimate the geometries that they present through the use of the high resolution DIP. The Phantom 4 Advanced equipped with RGB 1’’ CMOS effective 20 M sensor were the UAV model type and camera used for conducting the flight plan. The Pix4D Capture was the tablet/smartphone application used for conducting the flight operation and image collection in an area with ​​80 ha. After this step, the images were submitted as DIP routines using the SfM technique from the Agisoft Metashape software, version 1.5.1. The DIP is divided into stages like point cloud calculation, 3D models generation from mesh and texture procedures, digital elevation model (DEM) and orthomosaic. With the integration of images (DEM and orthomosaic) it was possible to identify and delineate a total of 14 targets through geometric information such as surface area, length, width, depth and internal format. The configuration in relation to soil type, vegetation and rock outcrops was the same around the Lagoa das Abelhas fossil tank. After that, the team came back to fieldwork and found fossil fragments of three out of fourteen targets. Thus, this study could show the potential of using UAV to cover large areas directed to the prospecting part of fossiliferous tanks with good flight autonomy, low cost and fast data analysis. Some of the 11 targets can be prospected because they have a high prospective potential due to their similarity to past prospects which became sites for future paleontological prospection.</p>


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