Low-cost system for electron probe microanalysis based on a personal computer

1990 ◽  
Vol 48 (2) ◽  
pp. 158-159
Author(s):  
Margaret C. Foster ◽  
Albert J. Saubermann
Keyword(s):  
Data Acquisition ◽  
Personal Computer ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Low Cost ◽  
Personal Computers ◽  
X Ray ◽  
Freeze Dried ◽  
The Cost ◽  
Data Acquisition And Processing

The development of personal computers increases the options available for electron probe microanalysis. Hardware and software are now available for personal computers which make it feasible to use them to acquire, process, and analyze x-ray spectra. One advantage of a personal computer (PC) based system is the low cost--approximately 20% of the cost of other options. A second advantage is that a system can be developed which is tailored to the needs of the laboratory, so that experimental questions asked of the data can dictate procedures for data acquisition and processing.We have developed a PC-based system for electron probe microanalysis, which we use for data acquisition, processing, and analysis of frozen and freeze-dried biological samples. X-ray spectra may be acquired either for spot analysis or for elemental images. Spectra acquired from large areas of the frozen, hydrated sample are used together with spectra from the freeze-dried specimen to calculate concentrations relative to sample wet weight.

scholarly journals STANDARDS FOR ELECTRON PROBE MICROANALYSIS OF BIOLOGIC SPECIMENS

1972 ◽  
Vol 20 (9) ◽  
pp. 710-715 ◽  
Author(s):  
G. M. LEHRER ◽  
C. BERKLEY
Keyword(s):  
Tissue Section ◽  
Elemental Analysis ◽  
Electron Microprobe ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Regression Curve ◽  
X Ray ◽  
Tissue Sections ◽  
Freeze Dried ◽  
Carbon Coated

A procedure is described for the preparation and application of gelatin standards in the quantitative elemental analysis of microscopic portions of tissue sections in the electron microprobe x-ray spectrometer. Ten to fifteen per cent gelatin solutions containing varying concentrations of the elements to be measured are quick-frozen in capsules, sectioned in a cryostat at the same thickness as the tissue and freeze-dried. Standard sections are mounted with each tissue section, carbon-coated and analyzed simultaneously. Concentrations of the elements in portions of the tissue as small as 10–12 liters are then determined directly from a regression curve or equation derived from the standards.

Reduction of Potassium in Kidney Proximal Tubules to Aid in Electron Probe X-Ray Microanalysis of Calcium

1985 ◽  
Vol 43 ◽  
pp. 474-475
Author(s):  
A. LeFurgey ◽  
P. Ingram ◽  
L.J. Mandel
Keyword(s):  
Smooth Muscle ◽  
Proximal Tubule ◽  
Electron Probe ◽  
Clinical Applications ◽  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Target Cells ◽  
X Ray ◽  
Freeze Dried

For quantitative determination of subcellular Ca distribution by electron probe x-ray microanalysis, decreasing (and/or eliminating) the K content of the cell maximizes the ability to accurately separate the overlapping K Kß and Ca Kα peaks in the x-ray spectra. For example, rubidium has been effectively substituted for potassium in smooth muscle cells, thus giving an improvement in calcium measurements. Ouabain, a cardiac glycoside widely used in experimental and clinical applications, inhibits Na-K ATPase at the cell membrane and thus alters the cytoplasmic ion (Na,K) content of target cells. In epithelial cells primarily involved in active transport, such as the proximal tubule of the rabbit kidney, ouabain rapidly (t1/2= 2 mins) causes a decrease2 in intracellular K, but does not change intracellular total or free Ca for up to 30 mins. In the present study we have taken advantage of this effect of ouabain to determine the mitochondrial and cytoplasmic Ca content in freeze-dried cryosections of kidney proximal tubule by electron probe x-ray microanalysis.

scholarly journals Early X-ray Mapping in Electron Probe Microanalysis

2006 ◽  
Vol 12 (S02) ◽  
pp. 1376-1377
Author(s):  
P Duncumb
Keyword(s):  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
X Ray

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005

scholarly journals A DIY Low-Cost Wireless Wind Data Acquisition System Used to Study an Arid Coastal Foredune

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 1064
Author(s):  
Antonio C. Domínguez-Brito ◽  
Jorge Cabrera-Gámez ◽  
Manuel Viera-Pérez ◽  
Eduardo Rodríguez-Barrera ◽  
Luis Hernández-Calvento
Keyword(s):  
Data Acquisition ◽  
Low Cost ◽  
Local Level ◽  
Environmental Studies ◽  
Data Acquisition System ◽  
Acquisition System ◽  
Wind Data ◽  
General Level ◽  
Dune Field ◽  
The Cost

Environmental studies on coastal dune systems are faced with a considerable cost barrier due to the cost of the instrumentation and sensory equipment required for data collection. These systems play an important role in coastal areas as a protection against erosion and as providers of stability to coastal sedimentary deposits. The DIY (Do-It-Yourself) approach to data acquisition can reduce the cost of these environmental studies. In this paper, a low-cost DIY wireless wind data acquisition system is presented which reduces the cost barrier inherent to these types of studies. The system is deployed for the analysis of the foredune of Maspalomas, an arid dune field situated on the south coast of Gran Canaria (Canary Islands, Spain), for the specific purpose of studying the dynamics of a dune type (tongue dunes), which is typical of this environment. The results obtained can be of interest for the study of these coastal environments at both the local level, for the management of this particular dune field, and at the general level for other similar dune fields around the world.

Synthetic TRPO4 crystals as reference samples in the quantitative X-ray electron probe microanalysis of rare-earth elements

2011 ◽  
Vol 66 (9) ◽  
pp. 831-837 ◽  
Author(s):  
Yu. G. Lavrent’ev ◽  
I. M. Romanenko ◽  
M. P. Novikov ◽  
L. V. Usova ◽  
V. N. Korolyuk
Keyword(s):  
Rare Earth ◽  
Rare Earth Elements ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
X Ray ◽  
Reference Samples
Sign in / Sign up

Export Citation Format

Share Document