Electron microscopy study of skeletal muscle microvasculature in the paraneoplastic phenomenon

In addition to signs and symptoms at sites of primary and metastatic disease, cancer can cause manifestations in remote sites that are not directly affected by any focus of disease. Such manifestations of malignancy are collectivelly known as paraneoplastic phenomenon. Muscular manifestations consist of slight to severe and usually proximal wasting and weakness. In our laboratory we described the ultrastructural pathology of skeletal muscle in the paraneoplastic phenomenon in patients with bronchogenic and cervix carcinoma. In these studies a brief report on capillary alterations was made. The present investigation was undertaken in order to extent the study on the microvasculature in the skeletal muscle paraneoplastic phenomenon.Muscle biopsies were obtained in patients suffering from bronchogenic carcinoma (n= 10), gastric carcinoma (n= 7), cervix carcinoma (n= 1) and lymphoma (n= 4). Tissue samples were processed by routine transmission electron microscope techniques and observed in a Hitachi H-500 EM.

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Inclusion body myositis: Electron microscopy study of 9 cases

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084946 ◽

1991 ◽

Vol 49 ◽

pp. 128-129

Author(s):

U.P. Kalyan-Raman ◽

Krishna Kalyan-Raman ◽

R.C. Caughey

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Inclusion Body ◽

Inclusion Body Myositis ◽

Paraffin Section ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Transmission Electron ◽

Paracrystalline Inclusions ◽

Muscle Biopsies

On routine examination of 266 muscle biopsies by histology, histochemistry and transmission electron microscopy, we diagnosed 9 cases of inclusion body myositis (IBM). We analyzed all the ultrastructural features present in these cases as to the pre-dictability of them in diagnosing IBM. All 9 cases shared common transmission electron microscopy features, such as: intranuclear filaments (Fig. 1), filaments within muscle fibers (Fig. 2), membranous whorls (rimmed vacuoles), cytoplasmic bodies (Fig. 3), mitochondrial accumulation and paracrystalline inclusions (Fig. A), honeycomb structures (Fig. 5), and atrophic fibers (Fig 6).266 muscle biopsies were studied. The biopsy was obtained by using the convential muscle clamp in all the cases. After getting the muscle biopsy in each case, about 1/3 of the length was frozen in liquid nitrogen at -170 degrees F to be used for histochemistry. The second 1/3 was fixed in formalin for paraffin section, and the remaining 1/3 was minced in small pieces and fixed in 2.5% glutaraldehyde for electron microscopy study.

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Significance of Nonspecific Structures Present in Diagnostic Muscle Biopsies in Electron Microscopy: A Study of 168 Cases

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158613 ◽

1990 ◽

Vol 48 (3) ◽

pp. 214-215

Author(s):

U.P. Kalyan-Raman ◽

R.C. Caughey

Keyword(s):

Electron Microscopy ◽

Muscle Biopsy ◽

Paraffin Section ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Routine Examination ◽

Transmission Electron ◽

Paracrystalline Inclusions ◽

Muscle Biopsies ◽

Fingerprint Bodies

On a routine examination of 168 muscle biopsies by transmission electron microscopy, we observed various structures, like filamentous bodies in 49 cases (29%), tubular aggregates in 22 (13%), paracrystalline inclusions in 15 (9%), honeycomb arrays in 11 (6.5%), concentric laminated bodies in 10 (6%), fingerprint bodies in 6 (3.8%), and fibrous nuclear inclusions in 3 (1.8%), for a total of 116/168 or a 70% occurrence. In order to evaluate the significance and occurrence of these structures in muscle biopsies, we correlated the clinical features, light microscopic and histochemical findings on these patients.168 muscle biopsies were studied. The muscle biopsy was obtained by using the conventional muscle clamp in all the cases. After obtaining the muscle biopsy in each case, about 1/3 of the length was frozen in liquid nitrogen at -170 degrees F to be used for histochemistry. The second 1/3 was fixed in formalin for paraffin section, and the remaining 1/3 was minced in small pieces and fixed in 2.5% glutaraldehyde for electron microscopy study.

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Factors influencing adhesive bonding at the bone/implant interface

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130201 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1114-1115

Author(s):

Julie A. Martini ◽

Robert H. Doremus

Keyword(s):

Electron Microscopy ◽

Adhesive Bonding ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Bone Implant ◽

Factors Influencing ◽

Lr White ◽

Transmission Electron ◽

New Zealand White Rabbits ◽

Scanning Electron

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.

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Transmission Electron Microscopy study of Bi-based superconducting phases

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100173388 ◽

1990 ◽

Vol 48 (4) ◽

pp. 50-51

Author(s):

J.G. Wen ◽

K.K. Fung

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Single Crystals ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Space Groups ◽

Superconducting Phases ◽

Ceramic Samples ◽

Transmission Electron ◽

Modulated Structures

Bi-based superconducting phases have been found to be members of a structural series represented by Bi2Sr2Can−1Cun−1On+4, n=1,2,3, and are referred to as 2201, 2212, 2223 phases. All these phases are incommensurate modulated structures. The super space groups are P2/b, NBbmb 2201, 2212 phases respectively. Pb-doped ceramic samples and single crystals and Y-doped single crystals have been studied by transmission electron microscopy.Modulated structures of all Bi-based superconducting phases are in b-c plane, therefore, it is the best way to determine modulated structure and c parameter in diffraction pattern. FIG. 1,2,3 show diffraction patterns of three kinds of modulations in Pb-doped ceramic samples. Energy dispersive X-ray analysis (EDAX) confirms the presence of Pb in the three modulated structures. Parameters c are 3 0.06, 38.29, 30.24Å, ie 2212, 2223, 2212 phases for FIG. 1,2,3 respectively. Their average space groups are all Bbmb.

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