Characterization of pseudorabies virus of wild boar origin from Europe

2010 ◽  
Vol 138 (11) ◽  
pp. 1590-1600 ◽  
Author(s):  
T. MÜLLER ◽  
B. G. KLUPP ◽  
C. FREULING ◽  
B. HOFFMANN ◽  
M. MOJCICZ ◽  
...  
Keyword(s):  
Wild Boar ◽  
Pseudorabies Virus ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Glycoprotein C ◽  
Hunting Dogs ◽  
Clade B ◽  
European Wild Boar ◽  
Causative Agents

SUMMARYPseudorabies virus (PrV) infections appear to be more widely distributed in the European wild boar (Sus scrofa) population than assumed. In Europe, attempts to isolate and characterize the causative agents have been limited so far. We therefore collected and examined a total of 35 PrV isolates obtained from wild boar or hunting dogs in Germany, France, Spain, Italy, Slovakia and Hungary between 1993 and 2008. Restriction enzyme analysis of genomic DNA using BamHI showed that all isolates, except one, belonged to genogroup I but different subtypes were evident. For further investigations of the phylogenetic relationships, a 732-bp fragment of the glycoprotein C (gC) gene was amplified by PCR. Sequence analysis revealed about 40 variant positions within this fragment. Comparison of the nucleotide sequences supported the separation into a clade containing isolates from North-Rhine Westphalia, Rhineland-Palatinate (Germany), France and Spain (clade B) and an apparently more variable clade comprising isolates from Brandenburg, Baden-Wurttemberg, Saxony, Saxony-Anhalt (Germany), Slovakia, Hungary, Italy and France (clade A).

Characterization of Trypanosoma cruzi isolates from Paraguay, using restriction enzyme analysis of kinetoplast DNA

1992 ◽  
Vol 86 (3) ◽  
pp. 231-237 ◽  
Author(s):  
T. Mimori ◽  
M. Maldonado ◽  
M. Samudio ◽  
A. Rojas De Arias ◽  
R. Moreno ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Kinetoplast Dna

scholarly journals Characterization of two BHV-4 strains isolated in the Czech Republic

2010 ◽  
Vol 55 (No. 3) ◽  
pp. 106-112 ◽  
Author(s):  
V. Fichtelova ◽  
K. Kovarcik
Keyword(s):  
Reference Strain ◽  
Fragment Size ◽  
Blot Hybridization ◽  
Size Variation ◽  
Southern Blot Hybridization ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
The Czech Republic ◽  
Herpesvirus 4

This study describes the isolation of bovine herpesvirus 4 (BHV-4) from the respiratory tract of animals suffering from respiratory disease. DNA of new isolates, CH and Ni, was cleaved with <I>Bam</I>HI, <I>Eco</I>RI and <I>Hind</I>III in restriction enzyme analysis and the fragments were identified by co-migration with the restriction profile of the reference strain Movar 33/63 cleaved with the appropriate endonuclease. Typical profiles with polyrepetitive DNA (prDNA) fragments were detected. In order to localize the size variation within the obtained digestion fragments, Southern blot hybridization was performed. Differences between the isolates CH, Ni were localized in both the prDNAs and the unique central part of the genome and were restricted to fragment size variation.

scholarly journals Genetic and structural characterization of an amino acid dimorphism in glycoprotein Ib alpha involved in platelet transfusion refractoriness

Blood ◽  
1992 ◽  
Vol 79 (11) ◽  
pp. 3086-3090 ◽  
Author(s):  
M Murata ◽  
K Furihata ◽  
F Ishida ◽  
SR Russell ◽  
J Ware ◽  
...  
Keyword(s):  
Platelet Transfusion ◽  
Platelet Reactivity ◽  
Recombinant Expression ◽  
Structural Basis ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Definitive Evidence ◽  
Naturally Occurring ◽  
Alpha Gene

Abstract The platelet-specific alloantigen, Siba, located within the alpha- subunit of the glycoprotein (GP) Ib-IX membrane receptor, has been found to be involved in the pathogenesis of platelet transfusion refractoriness. We have identified the existence of a naturally occurring threonine/methionine dimorphism at position 145 of the GPIb alpha sequence, and determined that the Siba antigen corresponds to the molecule containing methionine145. The diallelic codons can be detected by restriction enzyme analysis of amplified genomic DNA fragments from the GPIb alpha gene. Evaluation of 61 healthy blood donors showed that the allele frequencies are 89% and 11% for the threonine145 and methionine145 codons, respectively. A positive correlation exists between platelet reactivity with the anti-Siba antibody and the presence of a methionine145-encoding allele. Moreover, recombinant expression of two soluble GPIb alpha fragments differing only at residue 145, provided definitive evidence that the human anti-Siba antibody reacts only with the molecule containing methionine145. These results explain the structural basis of the Siba human alloantigen system and define screening methodologies useful in transfusion medicine to match donor and recipient platelets accordingly.

Detection and molecular characterization of Suid herpesvirus type 1 in Austrian wild boar and hunting dogs

2012 ◽  
Vol 157 (3-4) ◽  
pp. 276-284 ◽  
Author(s):  
Adolf Steinrigl ◽  
Sandra Revilla-Fernández ◽  
Jolanta Kolodziejek ◽  
Eveline Wodak ◽  
Zoltán Bagó ◽  
...  
Keyword(s):  
Wild Boar ◽  
Molecular Characterization ◽  
Herpesvirus Type ◽  
Hunting Dogs

Characterization of the DNA of Canine Adenovirus by Restriction Enzyme Analysis

Intervirology ◽  
1985 ◽  
Vol 23 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Gholamreza Darai ◽  
Angela R&ouml;sen ◽  
Hajo Delius ◽  
Rolf M. Fl&uuml;gel
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis

scholarly journals Molecular and Pathotypic Characterization of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Indian Chickens

2020 ◽  
Author(s):  
Dasharath B. Shinde ◽  
Anil L Thormoth ◽  
Santosh S. Koratkar ◽  
Neeti Sharma ◽  
Ashok Rajguru ◽  
...  
Keyword(s):  
Inclusion Body ◽  
Broiler Chickens ◽  
Emerging Diseases ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Hydropericardium Syndrome ◽  
Inclusion Body Hepatitis ◽  
Infective Dose ◽  
Pathogenic Analysis

Abstract Inclusion body hepatitis (IBH) is a disease of chickens characterized by acute mortality with severe anemia caused by fowl adenoviruses (FAdV). IBH is one of the most important re-emerging diseases world-wide. In India earlier IBH/ Hydropericardium Syndrome (HPS) outbreaks were mainly due to FAdV) type 4; however, in recent outbreaks, other serotypes are involved, despite regular vaccination in breeders and young chicks. The aim of the present study was isolation and molecular characterization of FAdV associated with IBH in India. A total of 193 liver samples of IBH suspected broiler chickens were collected from different regions of India. Liver samples were initially screened by histopathology and further, a total of 127 samples which microscopically showed basophilic, intra-nuclear inclusion bodies were processed for FAdV detection. A total of 69 samples were found to be positive for Hexon gene in PCR, of which 28 were sequenced. These samples showed more than 94% sequence homology with FAdV2 and 97% with FAdV11, which was confirmed by Restriction Enzyme Analysis (REA). Tissue Culture Infective Dose 50 (TCID50) and Egg Infective Dose50 (EID50) titer of isolated FAdV was found to be 106.5/ml. Pathogenicity study in SPF chicks showed 100% mortality up to Post Infection Day (PID)-6. These findings conclude that the prevalence of FAdV-11 is currently causing IBH outbreaks India. However, further genetic and pathogenic analysis of FAdV is required, which would provide useful information for the development of the efficient commercial IBH vaccine.

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