Molecular epidemiology ofBartonellaspecies isolated from ground squirrels and other rodents in northern California

2016 ◽  
Vol 144 (9) ◽  
pp. 1837-1844 ◽  
Author(s):  
A. C. ZIEDINS ◽  
B. B. CHOMEL ◽  
R. W. KASTEN ◽  
A. M. KJEMTRUP ◽  
C.-C. CHANG
Keyword(s):  
Rflp Analysis ◽  
Ground Squirrels ◽  
The Other ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Northern California ◽  
Wild Rodents ◽  
Neotoma Fuscipes ◽  
Pcr Rflp ◽  
California Ground Squirrels

SUMMARYBartonellaspp. are endemic in wild rodents in many parts of the world. A study conducted in two northern California counties (Sonoma and Yolo) sampling California ground squirrels (Otospermophilus beecheyi) and four other rodent species (Peromyscus maniculatus, P. boylii, P. trueiandNeotoma fuscipes) led to the isolation of small Gram-negative bacilli which were identified asBartonellaspp. based on colony morphology, polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and partial gene sequencing. Overall,Bartonellaspp. were isolated from the blood of 71% (32/45) of the ground squirrels and one third (22/66) of the other rodents. PCR–RFLP analysis of thegltA and 16S rRNA genes yielded seven unique profiles, four for the ground squirrels and three for the other rodents. Isolates from each PCR–RFLP profiles were submitted for partial sequencing. Ground squirrel isolates were most closely related toB. washoensis, whereas the other rodent isolates were closest toB. vinsoniisubsp.vinsoniiandB. vinsoniisubsp.arupensis. Two of these three species or subspecies are known zoonotic agents.

scholarly journals Coyotes (Canis latrans) as the Reservoir for a Human Pathogenic Bartonella sp.: Molecular Epidemiology ofBartonella vinsonii subsp. berkhoffii Infection in Coyotes from Central Coastal California

2000 ◽  
Vol 38 (11) ◽  
pp. 4193-4200 ◽  
Author(s):  
Chao-Chin Chang ◽  
Rickie W. Kasten ◽  
Bruno B. Chomel ◽  
Darren C. Simpson ◽  
Carrie M. Hew ◽  
...  
Keyword(s):  
16S Rrna ◽  
Canis Latrans ◽  
Bacterial Genome ◽  
Clinical Signs ◽  
Rflp Analysis ◽  
Domestic Dog ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Intergenic Spacer Region ◽  
Pcr Rflp

Bartonella vinsonii subsp. berkhoffii was originally isolated from a dog suffering infectious endocarditis and was recently identified as a zoonotic agent causing human endocarditis. Following the coyote bite of a child who developed clinical signs compatible with Bartonella infection in Santa Clara County, Calif., this epidemiological study was conducted. Among 109 coyotes (Canis latrans) from central coastal California, 31 animals (28%) were found to be bacteremic with B. vinsonii subsp.berkhoffii and 83 animals (76%) had B. vinsonii subsp. berkhoffii antibodies. These findings suggest these animals could be the wildlife reservoir of B. vinsonii subsp. berkhoffii. PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the gltA and 16S rRNA genes for these 31 isolates yielded similar profiles that were identical to those of B. vinsonii subsp.berkhoffii. Partial sequencing of the gltA and 16S rRNA genes, respectively, indicated 99.5 and 100% homology between the coyote isolate and B. vinsonii subsp.berkhoffii (ATCC 51672). PCR-RFLP analysis of the 16S-23S intergenic spacer region showed the existence of two different strain profiles, as has been reported in dogs. Six (19%) of 31Bartonella bacteremic coyotes exhibited the strain profile that was identified in the type strain of a canine endocarditis case (B. vinsonii subsp. berkhoffii ATCC 51672). The other 25 bacteremic coyotes were infected with a strain that was similar to the strains isolated from healthy dogs. Based on whole bacterial genome analysis by pulsed-field gel electrophoresis (PFGE) with SmaI restriction endonuclease, there was more diversity in fingerprints for the coyote isolates, which had at least 10 major variants compared to the two variants described for domestic dog isolates from the eastern United States. By PFGE analysis, threeBartonella bacteremic coyotes were infected by a strain identical to the one isolated from three healthy dog carriers. Further studies are necessary to elucidate the mode of transmission of B. vinsonii subsp. berkhoffii, especially to identify potential vectors, and to determine how humans become infected.

scholarly journals Molecular and Symptom Analyses of Phytoplasma Strains from Lettuce Reveal a Diverse Population

2004 ◽  
Vol 94 (8) ◽  
pp. 842-849 ◽  
Author(s):  
Jianhua Zhang ◽  
Saskia A. Hogenhout ◽  
Lowell R. Nault ◽  
Casey W. Hoy ◽  
Sally A. Miller
Keyword(s):  
Fragment Length Polymorphism ◽  
Phylogenetic Analyses ◽  
The Other ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Aster Yellows ◽  
Pcr Rflp ◽  
Horizontal Growth ◽  
Polymerase Chain ◽  
Leaf Distortion

Epidemics of aster yellows in lettuce in Ohio are caused by at least seven distinct phytoplasma strains in the aster yellows (AY) group. Five of the strains are newly reported: AY-BW, AY-WB, AY-BD3, AY-SS, and AY-SG. All seven strains were characterized based on symptoms in aster and lettuce, and by polymerase chain reaction (PCR). Strain AY-BD2 (formerly ‘Bolt’) causes yellowing and leaf distortion in lettuce and bolting in aster, whereas strain AY-S (formerly ‘Severe’) causes stunting, leaf clustering, and phyllody. Strain AY-WB causes yellowing and wilting in lettuce and witches'-broom in aster. Strain AY-SG induces horizontal growth in lettuce and aster plants. Strain AY-BW causes chlorosis of emerging leaves and abnormally upright growth of leaf petioles. AY-SS causes symptoms similar to those caused by AY-S but has a different PCR-restriction fragment length polymorphism (RFLP) banding pattern. Strains AY-BD2 and AY-BD-3 cause mild leaf and stem distortion in lettuce but are differentiated by PCR-RFLP. All phytoplasma strains collected from lettuce in Ohio belong to the 16SrI group. AY-WB belongs to the 16SrI-A subgroup and the other six belong to the 16SrI-B subgroup. Five of the seven strains were distinguished from each other by primer typing. The results of phylogenetic analyses of sequences of the 16S rRNA genes were basically consistent with the classification based on PCR-RFLP, in which AY-WB clustered with phytoplasmas of the 16rIA subgroup and the other Ohio lettuce strains clustered with phytoplasmas in the 16SrI-B subgroup.

PCR-RFLP analysis of fliC, fimH and 16S rRNA genes in Salmonella Typhimurium isolates of varied origin

2013 ◽  
Vol 64 (1) ◽  
pp. 177-183 ◽  
Author(s):  
Thangalazhy Gopakumar Sumithra ◽  
Vinod Kumar Chaturvedi ◽  
Praveen Kumar Gupta ◽  
Ajay Rai ◽  
Sunita Chougule ◽  
...  
Keyword(s):  
16S Rrna ◽  
Salmonella Typhimurium ◽  
Rflp Analysis ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Pcr Rflp

scholarly journals PCR-RFLP Analysis of 12s and 16s Mitochondrial rRNA Genes from Brackishwater Finfish and Shellfish Species

2005 ◽  
Vol 18 (1) ◽  
Author(s):  
M.S. SHEKHAR ◽  
G. GOPIKRISHNA ◽  
I.S. AZAD
Keyword(s):  
Genetic Variation ◽  
16S Rrna ◽  
Rflp Analysis ◽  
16S Rrna Genes ◽  
White Shrimp ◽  
Rrna Genes ◽  
Scylla Serrata ◽  
Aquatic Species ◽  
Asian Sea Bass ◽  
Pcr Rflp

The use of traditional genetic characterization techniques for detection of genetic variation in aquatic species based on morphological characters has its own limitations. One of the modern approaches to study genetic variation in aquatic species is by Restriction Fragment Length Polymorphism (RFLP) of mitochondrial DNA (mtDNA). Amplification of mitochondrial 12s and 16s rRNA genes from tiger shrimp (Penaeus monodon, Penaeidae), white shrimp (Fenneropenaeus indicus, Penaeidae), grey mullet (Mugil cephalus, Mugilidae), tilapia (Oreochromis mossambicus, Cichlidae), Asian sea bass (Lates calcarifer, Centropomidae) and mud crabs (Scylla serrata and Scylla tranquebarica, Portunidae) and the characterization of the amplified PCR products by RFLP have been carried out in the present study. The use of the primers in the present study was found to be universal for amplification of mitochondrial 12s and 16s rRNA genes across the taxonomically different brackishwater species examined in this investigation. The amplified products of 12s and 16s rRNA mitochondrial gene segments obtained with these primers can be used for restriction digestion as an approach to obtain species-specific markers by PCR-RFLP analysis.

Differentiation Of Clarias Batrachus, C. Gariepinus And Heteropneustes Fossilis By Pcr-Sequencing Of Mitochondrial 16s Rrna Gene

2015 ◽  
Vol 41 (1) ◽  
pp. 51-58
Author(s):  
Mohammad Shamimul Alam ◽  
Hawa Jahan ◽  
Rowshan Ara Begum ◽  
Reza M Shahjahan
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Fish Larva ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Valuable Insight ◽  
Multiple Sequence ◽  
Pcr Rflp ◽  
Alternative Means

Heteropneustesfossilis, Clariasbatrachus and C. gariepinus are three major catfishes ofecological and economic importance. Identification of these fish species becomes aproblem when the usual external morphological features of the fish are lost or removed,such as in canned fish. Also, newly hatched fish larva is often difficult to identify. PCRsequencingprovides accurate alternative means of identification of individuals at specieslevel. So, 16S rRNA genes of three locally collected catfishes were sequenced after PCRamplification and compared with the same gene sequences available from othergeographical regions. Multiple sequence alignment of the 16S rRNA gene fragments ofthe catfish species has revealed polymorphic sites which can be used to differentiate thesethree species from one another and will provide valuable insight in choosing appropriaterestriction enzymes for PCR-RFLP based identification in future. Asiat. Soc. Bangladesh, Sci. 41(1): 51-58, June 2015

672 IDENTIFICATION OF POLYBACTERIAL 16S RRNA GENES FROM ASCITIC FLUIDS WITH T-RFLP ANALYSIS AND DIRECT SEQUENCING

2012 ◽  
Vol 56 ◽  
pp. S266
Author(s):  
S. Krohn ◽  
J. Hartmann ◽  
A. Brodzinski ◽  
A. Chatzinotas ◽  
S. Bohm ◽  
...  
Keyword(s):  
16S Rrna ◽  
Direct Sequencing ◽  
Rflp Analysis ◽  
16S Rrna Genes ◽  
Rrna Genes

scholarly journals Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene

2013 ◽  
Vol 61 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Boglárka Sellyei ◽  
Éva Ivanics ◽  
Tibor Magyar
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Pasteurella Multocida ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
The Other ◽  
Pcr Rflp ◽  
H Gene ◽  
Geographic Regions ◽  
Type Strains ◽  
Restriction Fragment Length

The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.

Phenotypic and molecular characterization of chickpea rhizobia isolated from different areas of Tunisia

2007 ◽  
Vol 53 (3) ◽  
pp. 427-434 ◽  
Author(s):  
Boulbaba L’taief ◽  
Bouaziz Sifi ◽  
Maher Gtari ◽  
Mainassara Zaman-Allah ◽  
Mokhtar Lachaâl
Keyword(s):  
Rflp Analysis ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Phenotypic Characteristics ◽  
Cicer Arietinum L ◽  
Mesorhizobium Ciceri ◽  
Length Polymorphisms ◽  
Reference Strains ◽  
Tolerance To Salinity

Several phenotypic markers were used in this study to determine the biodiversity of rhizobial strains nodulating Cicer arietinum L. in various areas of Tunisia. They include symbiotic traits, the use of 21 biochemical substrates, and tolerance to salinity and pH. In addition, restriction fragment length polymorphisms (RFLPs) of PCR-amplified 16S rDNA were compared with those of reference strains. Numeric analysis of the phenotypic characteristics showed that the 48 strains studied fell into three distinct groups. This heterogeneity was highly supported by the RFLP analysis of 16S rRNA genes, and two ribotypes were identified. Chickpea rhizobia isolated from Tunisian soils are both phenotypically and genetically diverse. Results showed that 40 and 8 isolates were assigned, respectively, to Mesorhizobium ciceri and Mesorhizobium mediterraneum .

scholarly journals Analysis of the Endophytic Actinobacterial Population in the Roots of Wheat (Triticum aestivum L.) by Terminal Restriction Fragment Length Polymorphism and Sequencing of 16S rRNA Clones

2004 ◽  
Vol 70 (3) ◽  
pp. 1787-1794 ◽  
Author(s):  
Vanessa M. Conn ◽  
Christopher M. M. Franco
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Restriction Fragment Length

ABSTRACT The endophytic actinobacterial population in the roots of wheat grown in three different soils obtained from the southeast part of South Australia was investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis of the amplified 16S rRNA genes. A new, validated approach was applied to the T-RFLP analysis in order to estimate, to the genus level, the actinobacterial population that was identified. Actinobacterium-biased primers were used together with three restriction enzymes to obtain terminal restriction fragments (TRFs). The TRFs were matched to bacterial genera by the T-RFLP Analysis Program, and the data were analyzed to validate and semiquantify the genera present within the plant roots. The highest diversity and level of endophytic colonization were found in the roots of wheat grown in a dark loam from Swedes Flat, and the lowest were found in water-repellent sand from Western Flat. This molecular approach detected a greater diversity of actinobacteria than did previous culture-dependent methods, with the predominant genera being Mycobacterium (21.02%) in Swedes Flat, Streptomyces (14.35%) in Red Loam, and Kitasatospora (15.02%) in Western Flat. This study indicates that the soil that supported a higher number of indigenous organisms resulted in wheat roots with higher actinobacterial diversity and levels of colonization within the plant tissue. Sequencing of 16S rRNA clones, obtained using the same actinobacterium-biased PCR primers that were used in the T-RFLP analysis, confirmed the presence of the actinobacterial diversity and identified a number of Mycobacterium and Streptomyces species.

Sign in / Sign up

Export Citation Format

Share Document