Exploratory content analysis of direct-to-consumer pet genomics: What is being marketed and what are consumers saying?

Mars Petcare introduced the first direct-to-consumer domestic dog genetic test in 2009 and Basepaws introduced the first direct-to-consumer cat genetic test in 2016. Social science research has evaluated numerous aspects of the human direct-to-consumer market, yet no such exploration has evaluated the occurrence of pet owners pursuing pet genetic tests. Using a mixed methods approach, we conducted an exploratory content analysis of direct-to-consumer pet genetic company webpages and consumer reviews shared on Amazon. Initial data reviews indicated some companies may be key industry players, relative to others. Our results present content frequency for each group (key industry players, all other companies), though the primary themes for each remained the same. Analysis showed genetic companies are primarily sharing product and purchasing information, along with trustworthiness to establish the merit of the company and their products. Companies also used statements directed towards pet owners that are suggestive of both pets and “pet parents” benefiting from the test results. The primary themes identified in consumer reviews involved consumers sharing their perception about the tests (e.g., accuracy), what aspects of the test results they focused on (e.g., breed information), and experiences with using the test (e.g., ease of use). Amazon reviews were primarily positive, though the companies with smaller review numbers had higher percentages of negative and ambiguous sentiments. Of interest, reviews most often indicated tests were being used to determine a pet’s breed identity, while companies most frequently promoted the health advantages of using their products. Reviews revealed some consumers respond to tests by sharing their pet’s results with someone or by altering their pet’s care. Considering these results in addition to the growing popularity of this industry and the advancements of genomic technology, further research is needed to determine the role pet genetic testing may have in society and on human-animal relationships.

Genetic Dissection of Temperament Personality Traits in Italian Isolates

Human personality (i.e., temperament and character) is a complex trait related to mental health, influenced by genetic and environmental factors. Despite the efforts performed during the past decades, its genetic background is only just beginning to be identified. With the aim of dissecting the genetic basis of temperament, we performed a Genome-Wide Association Study (GWAS) on Cloninger’s Temperament and Character Inventory in 587 individuals belonging to different Italian genetic isolates. Data analysis led to the identification of four new genes associated with different temperament scales, such as Novelty Seeking (NS), Harm Avoidance (HA), and Reward Dependence (RD). In detail, we identified suggestive and significant associations between: MAGI2 (highest p-value = 9.14 × 10−8), a gene already associated with schizophrenia and depressive disorder, and the NS–Extravagance scale; CALCB (highest p-value = 4.34 × 10−6), a gene likely involved in the behavioral evolution from wild wolf to domestic dog, and the NS–Disorderliness scale; BTBD3 (highest p-value = 2.152 × 10−8), a gene already linked to obsessive–compulsive disorder, and the HA–Fatigability scale; PRKN (highest p-value = 8.27 × 10−9), a gene described for early onset Parkinson’s disease, and the RD scale. Our work provides new relevant insights into the genetics of temperament, helping to elucidate the molecular basis of psychiatric disorders.

Use of orthopedic metallic blocked plate for correction of unilateral jaw fracture in domestic dog: case report / Relato de caso: uso de placa metálica ortopédica bloqueada para correção de fratura unilateral de mandíbula em cão doméstico

As fraturas de mandíbula são comuns na rotina do médico veterinário clinico de pequenos animais, o tratamento visa imobilizar a lesão para restabelecer a mobilidade funcional do osso bem como a alimentação e mastigação natural. A determinação da terapêutica está condicionada a alguns fatores: tipo de fratura, localização, presença de dentes, oclusão dentária, raça e idade. Não há uma técnica de escolha ideal para todas fraturas, pois cada caso tem que ser analisado, levando em consideração os diversos fatores para utilizar o tratamento mais efetivo para determinada situação. Os novos tipos de materiais e tecnologias digitais são tendências atuais que permitirão otimizar os protocolos de tratamento de fraturas de mandíbula. Não há uma técnica de escolha, portanto, objetivou-se relatar um caso clinico cirúrgico de um cão, macho de 3 anos de idade e com fratura unilateral do ramo horizontal do corpo mandibular direito. A metodologia de escolha para o tratamento foi a utilização de placa bloqueada e fixação por osteossíntese cirúrgica. Após 90 dias de tratamento clinico e cirúrgico, o animal foi liberado de seu tratamento em perfeito estado de saúde.

Lyssaviruses and the Fatal Encephalitic Disease Rabies

Lyssaviruses cause the disease rabies, which is a fatal encephalitic disease resulting in approximately 59,000 human deaths annually. The prototype species, rabies lyssavirus, is the most prevalent of all lyssaviruses and poses the greatest public health threat. In Africa, six confirmed and one putative species of lyssavirus have been identified. Rabies lyssavirus remains endemic throughout mainland Africa, where the domestic dog is the primary reservoir – resulting in the highest per capita death rate from rabies globally. Rabies is typically transmitted through the injection of virus-laden saliva through a bite or scratch from an infected animal. Due to the inhibition of specific immune responses by multifunctional viral proteins, the virus usually replicates at low levels in the muscle tissue and subsequently enters the peripheral nervous system at the neuromuscular junction. Pathogenic rabies lyssavirus strains inhibit innate immune signaling and induce cellular apoptosis as the virus progresses to the central nervous system and brain using viral protein facilitated retrograde axonal transport. Rabies manifests in two different forms - the encephalitic and the paralytic form - with differing clinical manifestations and survival times. Disease symptoms are thought to be due mitochondrial dysfunction, rather than neuronal apoptosis. While much is known about rabies, there remain many gaps in knowledge about the neuropathology of the disease. It should be emphasized however, that rabies is vaccine preventable and dog-mediated human rabies has been eliminated in various countries. The global elimination of dog-mediated human rabies in the foreseeable future is therefore an entirely feasible goal.

Drivers of Predator Killing by Rural Residents and Recommendations for Fostering Coexistence in Agricultural Landscapes

Predators inhabiting human-dominated landscapes are vulnerable to various anthropogenic actions, including people killing them. We assess potential drivers of predator killing in an agricultural landscape in southern Chile, and discuss the implications for policies and interventions to promote coexistence. We evaluate five different types of motivation: (i) sociodemographics and household economy; (ii) livestock loss; (iii) predator encounter rates; (iv) knowledge of legal protection (all native predators are currently protected); and, (v) tolerance to livestock predation. As the killing of native predators is illegal, the prevalence of this behavior by rural residents was estimated using a symmetrical forced-response randomized response technique (RRT), a method designed to ask sensitive questions. A total of 233 rural residents from randomly assigned sample units (4 km2) across the study region completed our questionnaire. More conspicuous species, such as hawks (Falconiformes sp), foxes (Lycalopex sp) and free-roaming domestic dogs (Canis lupus familiaris), were killed by a higher proportion of farmers than more cryptic species, like the felid güiña (Leopardus guigna), skunk (Conepatus chinga) and pumas (Puma concolor). The proportion of respondents admitting to killing predators was highest for hawks (mean = 0.46, SE = 0.08), foxes (mean = 0.29, SE = 0.08) and dogs (mean = 0.30, SE = 0.08) and lowest for güiña (mean = 0.10, SE = 0.09), which is the only species of conservation concern we examine (considered Vulnerable on the IUCN Red List). From our five motivation categories, past killing of predators was associated with higher reported predator encounter rates (guina, hawks), lower tolerance to livestock predation (hawks, dogs), higher reported livestock loss (dogs) and sociodemographics and household economy (foxes). Our results demonstrate that a one-size-fits-all approach to predator persecution is unlikely to reduce or eliminate illegal killings for the suite of species we examined. We identify and describe two main types of intervention that could foster coexistence, improvement of livestock management and domestic dog management in rural areas, as well as discussing the potential for social marketing.

Between the Devil and the Deep Blue Sea: Non-Coding RNAs Associated with Transmissible Cancers in Tasmanian Devil, Domestic Dog and Bivalves

Currently there are nine known examples of transmissible cancers in nature. They have been observed in domestic dog, Tasmanian devil, and six bivalve species. These tumours can overcome host immune defences and spread to other members of the same species. Non-coding RNAs (ncRNAs) are known to play roles in tumorigenesis and immune system evasion. Despite their potential importance in transmissible cancers, there have been no studies on ncRNA function in this context to date. Here, we present possible applications of the CRISPR/Cas system to study the RNA biology of transmissible cancers. Specifically, we explore how ncRNAs may play a role in the immortality and immune evasion ability of these tumours.

The Case of Atypical Sexual Attractiveness in a Male Domestic Dog—A Case Study

During the ovarian cycle in domestic dogs, females do not accept males during the first days of estrus but become attractive to males from the beginning of proestrus, with this attractiveness persisting until the end of the estrus phase. It is believed that increased estradiol is responsible for the female attractiveness to the males. In this paper we describe the case of strong, but atypical attractiveness of a castrated male to various, adult, intact males, influenced by the emitted semiochemical signals. Any significant changes in the level of hormones typically involved in the process connected with estrus and responsible for sexual arousal in the males were assessed. The case animal was a 4 year old castrated male Border Collie that was extremely attractive to various males, which presented high levels of sexual arousal, with intensive sniffing and licking of the preputial area, specific vocalization, increased salivation and, finally, mating attempts. Clinical examination of the castrated male revealed a lack of testes in the scrotum and abdominal cavity confirmed by USG. Laboratory tests indicated basal levels of estradiol, testosterone, and progesterone (15.23 pg/mL, <0.05 ng/mL, 0.25 ng/mL), and sex was confirmed via cytogenetic and molecular analysis. Chemical analysis (HS-SPME) of the urine indicated a huge similarity to the profile obtained from a bitch in estrus, with an elevated level of acetophenone, which has been previously postulated in the literature as being a characteristic of the estrus phase in female domestic dogs. This case presented very atypical sexual attractiveness, particularly when taking into account the basal levels of hormones which, according to current knowledge, are responsible for the creation of attractiveness. As a hypothesis requiring verification, we propose the idea of involvement of other hormones in the creation of incidental attractiveness or increased production of compounds responsible for attractiveness (sex pheromones) resulting from metabolic events unrelated to reproductive processes. To our knowledge it is the first described case presenting this phenomenon, which, with more detailed study, could shed new light on the process of creation of sexual attraction in the domestic dog.