scholarly journals Detection of HEV-specific antibodies in four non-human primate species, including great apes, from different zoos in Germany

2017 ◽  
Vol 146 (1) ◽  
pp. 119-124 ◽  
Author(s):  
C. SPAHR ◽  
T. KNAUF-WITZENS ◽  
L. DÄHNERT ◽  
M. ENDERS ◽  
M. MÜLLER ◽  
...  
Keyword(s):  
Pan Paniscus ◽  
World Monkey ◽  
Sandwich Elisa ◽  
Clinical Signs ◽  
Hepatitis E ◽  
Gorilla Gorilla ◽  
Primate Species ◽  
Negative Results ◽  
Mandrillus Leucophaeus ◽  
In Captivity

SUMMARYThe hepatitis E virus (HEV) has been described in humans and various animal species in different regions of the world. However, the knowledge on natural HEV infection in non-human primates and the corresponding risk of zoonotic transmission is scarce. To determine whether primates in captivity are affected by HEV infection, we investigated 259 individual sera of clinically healthy non-human primates of 14 species from nine German zoos. Using a commercial double-antigen-sandwich ELISA and a commercial IgG ELISA, 10 animals (3·9%) reacted positive in at least one assay. Three ape species and one Old World monkey species were among the seropositive animals: bonobo (Pan paniscus), gorilla (Gorilla gorilla gorilla), lar gibbon (Hylobates lar) and drill (Mandrillus leucophaeus). Testing for anti-HEV-IgM antibodies by commercial ELISA and for viral RNA by reverse-transcription real-time polymerase chain reaction resulted in negative results for all animals indicating the absence of acute HEV infections. In the past, no clinical signs of hepatitis were recorded for the seropositive animals. The results suggest that non-human primates in zoos can get naturally and subclinically infected with HEV or related hepeviruses. Future studies should evaluate potential sources and transmission routes of these infections and their impact on human health.

COVID-19: how nuclear medicine can provide a differential diagnosis in very dubious case

Coronaviruses ◽  
2020 ◽  
Vol 01 ◽  
Author(s):  
Maria Silvia De Feo ◽  
Viviana Frantellizzi ◽  
Giuseppe De Vincentis
Keyword(s):  
Infectious Disease ◽  
Differential Diagnosis ◽  
Ct Scan ◽  
Imaging Technique ◽  
False Negative ◽  
Clinical Signs ◽  
Negative Results ◽  
Infectious Disease Department ◽  
False Negative Results ◽  
99Mtc Hmpao

Background: We present the case of a 55-year-old woman, admitted to the Infectious Disease Department of Policlinico Umberto I, Rome, in mid-March 2020, with suspicion of COVID-19 infection. Objective: The rRT-PCR was negative and the following CT scan, performed to exclude false-negative results and help diagnosis, was inconclusive. Methods: It was decided to submit the patient to 99mTc-HMPAO-labelled leukocyte scan. Results: This exam led to the diagnosis of infective endocarditis. Conclusion: In the present pandemic scenario, 99mTc-HMPAO-labelled leukocyte scan represents a reliable imaging technique for differential diagnosis with COVID-19 in patients with confusing clinical signs, possible false-negative rRT-PCR results and inconclusive CT scan.

FEMALE DOMINANCE RELATIONSHIPS AMONG CAPTIVE WESTERN LOWLAND GORILLAS: COMPARISONS WITH THE WILD

Behaviour ◽  
1999 ◽  
Vol 136 (10-11) ◽  
pp. 1283-1310 ◽  
Author(s):  
Joan Lockard ◽  
Jennifer Scott
Keyword(s):  
Natural Habitat ◽  
High Energy ◽  
Gorilla Gorilla ◽  
Female Dominance ◽  
Primate Species ◽  
Dominance Hierarchies ◽  
Mountain Gorillas ◽  
Dominance Relationships ◽  
Western Lowland Gorillas ◽  
Lowland Gorillas

AbstractFemale dominance relationships were studied among three family groups of western lowland gorillas (Gorilla gorilla gorilla) housed in large enclosures at Howletts Wild Animal Park, in Kent, England. In common with gorillas in their natural habitat, the Howletts gorillas forage throughout the day on low nutrient foods. However, the latter differ, at least from mountain gorillas (Gorilla gorilla beringei), in that they also have relatively frequent access to high nutrient, high energy novel food items which are patchily distributed in time and space, and defendable. It was predicted that, despite these differences, the Howletts females would resemble mountain gorillas in forming adult female dominance hierarchies (determined from supplant interactions) in which older females that have lived in the group the longest are dominant to younger females, more recent to the group. The comparison was made with mountain gorillas as they are the only gorilla subspecies for which such data exist for wild-living populations. As predicted, an age/tenure-based dominance hierarchy was found to be the case for those groups at Howletts where there was considerable variation between the females' ages and length of group tenure. As gorillas and chimpanzees resemble more closely each other in forming age/tenure-based dominance hierarchies than they do other female-transfer primate species, it is proposed that the gorilla-chimpanzee pattern may have common phylogenetic origins. In addition, the order of progression of gorillas into their indoor living quarters appears to be a good indicator of supplant-dominance relationships among adult group members. It was also found that, despite being removed from their natural habitat, dominant males in captivity still lead their groups during group travel in the same manner that do males in the wild: either at the head, or bringing up the rear. Though primate social behaviour may be flexible depending on immediate context and life history variables, this flexibility may well remain within evolutionarily defined parameters, leading to species-typical patterns in general social interactions.

scholarly journals Viral hepatitis E: A disease of humans and animals

2012 ◽  
Vol 66 (5-6) ◽  
pp. 449-462
Author(s):  
Branislav Kureljusic ◽  
Vojin Ivetic ◽  
Bozidar Savic ◽  
Jasna Kureljusic ◽  
Nemanja Jezdimirovic
Keyword(s):  
Viral Hepatitis ◽  
Hepatitis E Virus ◽  
Clinical Signs ◽  
Hepatitis E ◽  
Developed Countries ◽  
Animal Origin ◽  
Oral Transmission ◽  
Source Of Infection ◽  
Pig Farms ◽  
The Usa

The hepatitis E virus is ubiquitous in all parts of the world where pig production exists. The infection occurs in several animal species and its course is mostly asymptomatic. Viral strains isolated from pigs and humans are genetically similar, which indicates a potential zoonotic nature of the disease, and the possibility that pigs, and perhaps also other species of animals diseased with viral hepatitis E are a source of infection to humans. The pig hepatitis E virus, which is similar to the hepatitis E virus in humans, was isolated and described for the first time in the USA in 1997. The infection of pigs with hepatitis E virus occurs through faeco-oral transmission, by ingestion of feed and water contaminated with the virus, or through direct contact between infected and healthy animals. The pathogenesis of this infection in pigs differs from its pathogenesis in humans and it has not been sufficiently examined in all its aspects. Even though viral hepatitis E in pigs has been described as a subclinical disease, some authors describe changes in the concentration of certain biochemical parameters in blood serum of the infected pigs. Histologically, a mild to moderate lymphotic-plasma cellular infiltration is observed in livers of infected pigs, as well as focal areas of hepatocyte necrosis. Viral hepatitis E is an endemic disease of humans in Asia, Africa, and Latin America. In developed countries, hepatitis E sporadically occurs in humans, but it is becoming of increasing importance in particular in Japan, North America, and Europe, because the populations of these areas travel extensively to the endemic regions or as a result of the consumption of thermally untreated meat of wild boar and products made from thermally untreated meat. Pork products can be contaminated with hepatitis E virus. Further proof that indicates the zoonotic potential of this virus and places this diseases among the group of professional diseases of farmers and veterinarians is the finding of antibodies to hepatitis E virus in farmers and veterinarians who work on pig farms without showing any clinical signs of the disease. Having in mind the fact that viral hepatitis E has been proven in pig farms in Serbia and neighboruign countries, there should be strict respect of biosecutiry measures from the episootiological and epidemiological aspects, and the principle of good production and hygiene practice should be adhered to on pig farms. This disease should in future also be included in the legal regulations of our country in order to ensure the production of products of animal origin that are safe from the aspect of hygiene.

scholarly journals CENP-B box, a nucleotide motif involved in centromere formation, occurs in a New World monkey

2016 ◽  
Vol 12 (3) ◽  
pp. 20150817 ◽  
Author(s):  
Aorarat Suntronpong ◽  
Kazuto Kugou ◽  
Hiroshi Masumoto ◽  
Kornsorn Srikulnath ◽  
Kazuhiko Ohshima ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
New World ◽  
World Monkey ◽  
Common Marmoset ◽  
Alpha Satellite ◽  
Satellite Repeat ◽  
New World Monkey ◽  
Alpha Satellite Dna ◽  
Negative Results ◽  
Contig Sequence

Centromere protein B (CENP-B) is one of the major proteins involved in centromere formation, binding to centromeric repetitive DNA by recognizing a 17 bp motif called the CENP-B box. Hominids (humans and great apes) carry large numbers of CENP-B boxes in alpha satellite DNA (AS, the major centromeric repetitive DNA of simian primates). Only negative results have been reported regarding the presence of the CENP-B box in other primate taxa. Consequently, it is widely believed that the CENP-B box is confined, within primates, to the hominids. We report here that the common marmoset, a New World monkey, contains an abundance of CENP-B boxes in its AS. First, in a long contig sequence we constructed and analysed, we identified the motif in 17 of the 38 alpha satellite repeat units. We then sequenced terminal regions of additional clones and found the motif in many of them. Immunostaining of marmoset cells demonstrated that CENP-B binds to DNA in the centromeric regions of chromosomes. Therefore, functional CENP-B boxes are not confined to hominids. Our results indicate that the efficiency of identification of the CENP-B box may depend largely on the sequencing methods used, and that the CENP-B box in centromeric repetitive DNA may be more common than researchers previously thought.

Cradle Under the Canopy The Forest Origins of our Ape and Hominin Ancestors and the Tropical Forest Forays of the Genus Homo

2019 ◽  
Author(s):  
Patrick Roberts
Keyword(s):  
Tool Use ◽  
Pan Paniscus ◽  
Great Apes ◽  
Last Common Ancestor ◽  
Great Ape ◽  
Tropical Environments ◽  
Genus Homo ◽  
In Captivity ◽  
Game Hunting ◽  
The Tropics

The evolutionary proximity of the non-human great apes to us is often stressed in studies of animals, such as Kanzi, a bonobo (Pan paniscus) bred in captivity, that demonstrate their capacity to undertake tool-use and even utilize and comprehend language (Toth et al., 1993; Savage-Rumbaugh and Lewin, 1996; Schick et al., 1999). Likewise, studies of chimpanzees (Pan spp.) have highlighted the similarity of their emotional and empathetic capacities to those of humans (Parr et al., 2005; Campbell and de Waal, 2014). However, as noted by Savage- Rumbaugh and Lewin (1996), in palaeoanthropology and archaeology more broadly, the emergence of the hominin clade and, later, our species, is referenced in terms of the ‘chasm’ between ourselves and other extant great apes. Indeed, despite our genetic and behavioural proximity, extant non-human great ape taxa are often popularly characterized as living fossils of how we used to be. They are used as analogues for the subsistence and behaviour of the Last Common Ancestor (LCA) of humans and non-human great apes (Clutton-Brock and Harvey, 1977; Goodall, 1986; Foley and Lewin, 2004) and it is almost as if the fact that they still occupy the tropical environments in which these hominoids likely evolved (though see Elton, 2008) allows them to be treated as static comparisons (Figure 3.1). Since Darwin wrote the Descent of Man in 1871, the forests of the tropics, and their modern non-human great ape inhabitants, have tended to be perceived as being left behind as the hominin clade gained increasingly ‘human’ traits of tool-use, medium to large game hunting, and upright locomotion on open ‘savanna’ landscapes (Dart, 1925; Potts, 1998; Klein, 1999). From this perspective it is perhaps unsurprising that tropical forests are seen as alien to the genus Homo and its closest hominin ancestors.

Sign in / Sign up

Export Citation Format

Share Document