scholarly journals Molecular characterization of Coxiella burnetii isolates

1998 ◽  
Vol 120 (2) ◽  
pp. 157-164 ◽  
Author(s):  
C. JÄGER ◽  
H. WILLEMS ◽  
D. THIELE ◽  
G. BALJER
Keyword(s):  
Coxiella Burnetii ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Geographical Origin ◽  
Pulsed Field Gel Electrophoresis ◽  
Evolutionary Relationships ◽  
Restriction Patterns ◽  
Typing System ◽  
Restriction Group

Restriction fragment length polymorphism (RFLP) was used for the differentiation of 80 Coxiella burnetii isolates derived from animals and humans in Europe, USA, Africa and Asia. After NotI restriction of total C. burnetii DNA and pulsed field gel electrophoresis (PFGE) 20 different restriction patterns were distinguished. The index of discrimination for this typing system was 0·86. Comparison and phylogenetic analysis of the different RFLP patterns revealed evolutionary relationships among groups that corresponded to the geographical origin of the isolates. This finding was confirmed by genetic mapping. No correlation between restriction group and virulence of isolates was detected.

Comparison of serotyping, pulsed field gel electrophoresis and amplified fragment length polymorphism for typing of Streptococcus pneumoniae

2005 ◽  
Vol 54 (5) ◽  
pp. 467-472 ◽  
Author(s):  
Aishath Shaaly ◽  
Marit Gjerde Tellevik ◽  
Nina Langeland ◽  
E Arne Høiby ◽  
Roland Jureen
Keyword(s):  
Streptococcus Pneumoniae ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Pulsed Field Gel Electrophoresis ◽  
Discriminatory Power ◽  
Western Norway ◽  
Typing System ◽  
Typing Methods ◽  
Good Concordance

The aim of the present study was to compare serotyping, PFGE and AFLP for typing of Streptococcus pneumoniae with regard to discriminatory power, typeability and typing system concordance. Thirty-four isolates from cerobrospinal fluid and 34 time-matched blood culture isolates collected from in-patients at two hospitals in western Norway during the period from January 1994 to May 2002 were included in the study. The discriminatory powers of serotyping, PFGE and AFLP were 0.93, 0.99 and 0.95, respectively. The typeabilities for serotyping, PFGE and AFLP were 1, 1 and 0.99, respectively. A good concordance was shown between all the typing methods. Serotyping would most probably have a higher discriminatory power if further subtyping had been performed. PFGE was more discriminatory than AFLP, and AFLP grouped more-distantly related isolates together. The two typing methods thus provided different information, and therefore both could be useful adjuncts to serotyping for the characterization of S. pneumoniae.

Characterization of Listeria monocytogenes on Retail Food from Jiangsu Province, China

2013 ◽  
Vol 781-784 ◽  
pp. 1332-1335
Author(s):  
Tao Yu
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Gel Electrophoresis ◽  
Susceptibility Testing ◽  
Pulsed Field Gel Electrophoresis ◽  
Restriction Patterns ◽  
Pulsed Field ◽  
Retail Food

Listeria monocytogenes isolates recovered from retail foods were characterized by serotyping, genotyping using pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Six serotypes were identified among the isolates and the predominant serotype was 1/2a (42, 48.8%). PFGE differentiated the 86 isolates into 23 ApaI restriction patterns. Twenty-five (29.1%) of the L.monocytogenes strains exhibited resistance to at least one antimicrobial. Resistance to ciprofloxacin (17.4% of the isolates were resistant) and resistance to tetracycline (17.4%) were observed frequently.

scholarly journals Characterization of Staphylococcus intermedius from Pigeons, Dogs, Foxes, Mink, and Horses by Pulsed-Field Gel Electrophoresis

2002 ◽  
Vol 64 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Yoshihisa WAKITA ◽  
Akira SHIMIZU ◽  
Václav HÁJEK ◽  
Junichi KAWANO ◽  
Kenji YAMASHITA
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Staphylococcus Intermedius

scholarly journals Application of pulsed-field gel electrophoresis to the epidemiological characterization ofStaphylococcus intermediusimplicated in a food-related outbreak

1994 ◽  
Vol 113 (1) ◽  
pp. 75-81 ◽  
Author(s):  
F. M. Khambaty ◽  
R. W. Bennett ◽  
D. B. Shah
Keyword(s):  
Gel Electrophoresis ◽  
Pfge Pattern ◽  
Pulsed Field Gel Electrophoresis ◽  
Western United States ◽  
Single Strain ◽  
Pulsed Field ◽  
Definitive Evidence ◽  
Staphylococcus Intermedius ◽  
Epidemiological Characterization

SUMMARYAn outbreak of food intoxication involving over 265 cases in western United States occurred in October 1991.Staphylococcus intermediuswas implicated as the aetiologic agent. Representative outbreak isolates (five clinical and ten from foods) produced type A enterotoxin. DNA fragments generated by four restriction endonucleases and analysed by pulsed-field gel electrophoresis (PFGE) provided definitive evidence that all isolates from nine different counties in California and Nevada were derived from a single strain. The PFGE pattern of these outbreak isolates was distinct from those of a heterogeneous collection of sevenS. intermediusstrains of veterinary origin and five unrelatedS. aureuslaboratory strains. The data show a significant PFGE pattern heterogeneity not only among members of differentStaphylococcusspecies but also within members of the same species and even the same enterotoxin type. The results indicate that PFGE is a valuable strain-specific discriminator for the epidemiological characterization ofS. intermedius. To our knowledge, this represents the first documented foodborne outbreak caused byS. intermedius. These findings suggest that the presence ofS. intermediusand other species such asS. hyicusin food should be reason for concern.

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