Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence

1990 ◽  
Vol 2 (2) ◽  
pp. 149-155 ◽  
Author(s):  
John C. Priscu ◽  
Linda R. Priscu ◽  
Anna C. Palmisano ◽  
Cornelius W. Sullivan
Keyword(s):  
Sea Ice ◽  
Neutral Lipid ◽  
Nile Red ◽  
Lipid Levels ◽  
Antarctic Sea Ice ◽  
Neutral Lipid Content ◽  
Scatter Plots ◽  
Phaeocystis Pouchetii ◽  
Nile Red Fluorescence ◽  
Platelet Ice

The fluorescent neutral lipid stain, nile red, was used to examine cell-specific neutral lipid levels in natural assemblages of Antarctic sea ice microalgae. Neutral lipid:chlorophyll, neutral lipid:particulate carbon (PC) and neutral lipid:particulate nitrogen (PN) ratios were highest in communities dominated by Nitzschia spp. and Navicula glaciei van Heurck. The lowest specific neutral lipid content was estimated in the congelation ice samples dominated by the diatom Amphiprora spp., and in surface assemblages dominated by Phaeocystis pouchetii Hariot and the dinoflagellate Gymnodinium sp. Scatter plots of neutral lipid on PC and PN, which included data from all assemblages, showed that assemblages dominated by P. pouchetii and Amphiprora spp. clustered near the origin reflecting their relatively lower specific neutral lipid levels, compared with assemblages dominated by N. glaciei and Nitzschia spp. Cellular PC:PN was significantly (P<0.001) lower in microalgae inhabiting surface melt pools or tide cracks compared to those associated with congelation or platelet ice.

Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red

1987 ◽  
Vol 6 (6) ◽  
pp. 333-345 ◽  
Author(s):  
Keith E. Cooksey ◽  
James B. Guckert ◽  
Scott A. Williams ◽  
Patrik R. Callis
Keyword(s):  
Neutral Lipid ◽  
Lipid Content ◽  
Nile Red ◽  
Fluorometric Determination ◽  
Neutral Lipid Content

OCCURRENCE OF AN ISOPRENOID C25 DIUNASATURATED ALKENE AND HIGH NEUTRAL LIPID CONTENT IN ANTRACTIC SEA-ICE DIATOM COMMUNITIES1

2008 ◽  
Vol 24 (1) ◽  
pp. 90-96 ◽  
Author(s):  
Peter D. Nichols ◽  
Anna C. Palmisano ◽  
John K. Volkman ◽  
Glen A. Smith ◽  
David C. White
Keyword(s):  
Sea Ice ◽  
Neutral Lipid ◽  
Lipid Content ◽  
Neutral Lipid Content

scholarly journals Neutral Lipid Content in Lipid Droplets: Potential Biomarker of Cordycepin Accumulation in Cordycepin-Producing Fungi

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3363 ◽  
Author(s):  
Peng Qin ◽  
ZhiYe Wang ◽  
DengXue Lu ◽  
HongMei Kang ◽  
Guang Li ◽  
...  
Keyword(s):  
Neutral Lipid ◽  
Fluorescence Intensity ◽  
Lipid Content ◽  
Lipid Droplets ◽  
Neutral Lipids ◽  
Nile Red ◽  
Hplc Method ◽  
Relative Fluorescence Intensity ◽  
Potential Biomarker ◽  
Neutral Lipid Content

To clarify the relationship between neutral lipid content and cordycepin accumulation in Cordyceps militaris, mutants were generated from mixed spores of two C. militaris strains with varying cordycepin-producing capacities. Fifteen stable mutants producing from 0.001 to 2.363 mg/mL cordycepin were finally selected. The relative fluorescence intensities of the 15 mutants, two C. militaris strains and an Aspergillus nidulans strain at different concentrations of lyophilized mycelium powder were then investigated using the Nile red method. The mutant CM1-1-1 with the highest relative fluorescence intensity among the eighteen strains was selected for optimizing the Nile red method. Relative fluorescence intensity was linearly correlated with cordycepin concentration in liquid broth (R2 = 0.9514) and in lyophilized mycelium powder (R2 = 0.9378) for the 18 cordycepin-producing strains under identical culture conditions and with cordycepin concentration in liquid broth (R2 = 0.9727) and in lyophilized mycelium powder (R2 = 0.9613) for CM1-1-1 under eight different sets of conditions. In addition, the cordycepin content in lyophilized mycelium powder measured by the Nile red method was linearly correlated with that determined by an HPLC method (R2 = 0.9627). In conclusion, neutral lipids in lipid droplets are required during cordycepin accumulation; these neutral lipids are potential biomarkers of cordycepin biosynthesis.

scholarly journals Growth of first-year landfast Antarctic sea ice determined from winter temperature measurements

2006 ◽  
Vol 44 ◽  
pp. 170-176 ◽  
Author(s):  
Craig R. Purdie ◽  
Patricia J. Langhorne ◽  
Greg H. Leonard ◽  
Tim G. Haskell
Keyword(s):  
Heat Flux ◽  
Sea Ice ◽  
Ice Cores ◽  
First Year ◽  
Conductive Heat ◽  
Ice Growth ◽  
Antarctic Sea Ice ◽  
Oceanic Heat Flux ◽  
Landfast Sea Ice ◽  
Platelet Ice

AbstractTemperature profiles of first-year landfast sea ice have been recorded continuously over the 2003 winter growth season at McMurdo Sound, Antarctica. The temperature gradients in the ice were used to calculate the growth rate due to conductive heat flux, which is shown to account for only part of the total ice growth. Remaining ice growth must be due to a negative oceanic heat flux. Significantly, this oceanic heat flux is shown to occur episodically, sometimes with sustained daily rates in excess of –30Wm–2. There is no direct correlation between oceanic heat flux and water temperature. Times of increased oceanic heat flux do coincide with the appearance of platelet ice in cores, and appear to account for the growth of 35% of the total platelet ice depth measured in ice cores.

Dissolved carbohydrates in Antarctic sea ice

2001 ◽  
Vol 13 (2) ◽  
pp. 119-125 ◽  
Author(s):  
L.-M. Herborg ◽  
D.N. Thomas ◽  
H. Kennedy ◽  
C. Haas ◽  
G.S. Dieckmann
Keyword(s):  
Sea Ice ◽  
Algal Biomass ◽  
Ice Cores ◽  
Weddell Sea ◽  
The Other ◽  
Surface Seawater ◽  
Antarctic Sea Ice ◽  
Ice Surface ◽  
Total Carbohydrates ◽  
Platelet Ice

Concentrations of dissolved monocarbohydrates (MCHO) and polycarbohydrates (PCHO) were analysed in a variety of ice habitats from summer Weddell Sea sea ice (surface ponds, ice cores, gap layers and platelet ice). The dissolved organic carbon (DOC) pool in these habitats was also measured and the contribution of carbohydrate to this pool was assessed. The DOC concentrations within all sea ice habitats were high compared to surface seawater concentrations with values up to 958μMC being measured. Total carbohydrates (TCHO) were highest in the ice cores and platelet ice samples, up to 31% of the DOC pool, a reflection of the high algal biomass in these two habitat classes. TCHO in the other habitats ranged between 10% and 29% of DOC. The ratios of MCHO to PCHO varied considerably between the ice habitats: in surface ponds and ice cores MCHO was 70% of the TCHO pool, whereas in gap layers and platelet ice there were lower PCHO concentrations resulting in MCHO being 88% of TCHO.

scholarly journals Observed platelet ice distributions in Antarctic sea ice: An index for ocean-ice shelf heat flux

2015 ◽  
Vol 42 (13) ◽  
pp. 5442-5451 ◽  
Author(s):  
P. J. Langhorne ◽  
K. G. Hughes ◽  
A. J. Gough ◽  
I. J. Smith ◽  
M. J. M. Williams ◽  
...  
Keyword(s):  
Heat Flux ◽  
Sea Ice ◽  
Ice Shelf ◽  
Antarctic Sea Ice ◽  
Platelet Ice
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