Taql growth hormone gene polymorphism and milk production traits in Holstein-Friesian cattle

1996 ◽  
Vol 63 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Falaki ◽  
M. Sneyers ◽  
A. Prandi ◽  
S. Massart ◽  
C. Corradini ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Restriction Fragment ◽  
Milk Fat ◽  
Substitution Effect ◽  
Growth Hormone Gene ◽  
Production Traits ◽  
Milk Production Traits ◽  
Frequency Pattern ◽  
Holstein Friesian

AbstractThe positive effect of administration of growth hormone (GH) on milk production and its presence in selected dairy cattle lines of higher GH concentrations prompted an examination of the presence of restriction fragment length polymorphism (RFLP) in the growth hormone gene using the enzyme Taq/ and to investigate associations between this polymorphism and milk production traits. Blood was sampled from 251 Italian Holstein-Friesian cows. Three fragment bands, arbitrarily denoted A, B and E, of 6·2, 5·2 and 1·9 kilobase (kb), respectively, were observed. Their combinations exhibited six patterns, AA, AB, ABE, AE, BB and BE with frequencies of 64·5, 24·3, 2·4, 6·8, 1·4 and 0·4%, respectively. The statistical analysis was performed using linear mixed animal models. The results indicated an effect of the GHgene polymorphic Taq/restriction fragment on 305-day productions of milk, fat and protein; the low frequency pattern AE showed productions inferior to those for AA or AB patterns. Effect estimates of AA, AB and AE were, respectively, 200 (s.e. 215), 218 (s.e. 267) and -910 (s.e. 380) kg for milk production, 7·75 (s.e. 7·98), 16·10 (s.e. 9·79) and -22·14 (s.e. 14·42) kg for fat production, and 6·78 (s.e. 6·21), 8·57 (s.e. 7·58) and -20·74 (s.e. 11·14) kg for protein production. The average substitution effect estimates of E were -891 (s.e. 278), -26·56 (s.e. 10·16) and -24·50 (s.e. 9·43) kg for milk, fat and protein yields, respectively. In conclusion, these results suggest that the E fragment deserves further designed and specific study.

scholarly journals Associations between <i>Leu/Val</i> polymorphism of growth hormone gene and milk production traits in Black-and-White cattle

2002 ◽  
Vol 45 (5) ◽  
pp. 421-428 ◽  
Author(s):  
A. Dybus
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Growth Hormone Gene ◽  
Production Traits ◽  
Milk Production Traits ◽  
Black And White ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
White Cattle

Abstract. Associations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production traits of Black-and-White cattle with different proportional share of Holstein Friesian genes were analysed. A total of 1086 cows were included in the study. PCR-RFLP method was used for genotyping. The frequencies of genotypes and alleles were as follows: 0.653 for LL genotype, 0.324 for LV and 0.023 for VV, and 0.815 for GHL and 0.185 for GHV. Associations between Leu/Val polymorphism and milk production traits of cows were found only in first lactation. Cows with LL genotype had higher milk, fat and protein yield compared to LV individuals (P ≤ 0.01).

scholarly journals The relation of GH1, GHR and DGAT1 polymorphisms with estimated breeding values for milk production traits of German Holstein sires

2008 ◽  
Vol 53 (No. 6) ◽  
pp. 238-246 ◽  
Author(s):  
E. Hradecká ◽  
J. Čítek ◽  
L. Panicke ◽  
V. Řehout ◽  
L. Hanusová
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Milk Protein ◽  
Growth Hormone Gene ◽  
Production Traits ◽  
Milk Production Traits ◽  
Breeding Values ◽  
Fat Yield ◽  
Estimated Breeding Values

: We analysed the relations of estimated breeding values (EBV) of 315 German Holstein sires to their genotypes in growth hormone gene (<i>GH1</i>), growth hormone receptor gene (<i>GHR</i>) and acylCoA-diacylglycerol acyltransferase 1 (<i>DGAT1</i>). The strong relation of <i>DGAT1 K232A</i> to the estimated breeding values for milk production traits has been confirmed, when allele <i>DGAT1<sup>K</sup></i> was connected with higher milk fat yield, milk fat and milk protein content, while allele <i>DGAT1<sup>A</sup></i> increased milk yield and milk protein yield. The effect of <i>DGAT1</i> genotype explained from 4.70% of variability of EBVs for fat yield to 31.90% of variability of EBVs for fat content. The evaluation of <i>GH1</i> 127 Leu/Val and <i>GHR</i> 257 SNP polymorphisms did not reveal an association of their polymorphism with EBVs for milk production traits, except the EBVs of <i>GHR<sup>G</sup>/GHR<sup>G</sup></i> homozygotes for fat yield, which were significantly lower. The effect of <i>GH1</i> or <i>GHR genotype explained only a negligible portion of variability of EBVs (<i>R</i><sup>2</sup> < 1.00% in most cases).

Association of the Leu127 variant of the bovine growth hormone (bGH) gene with increased yield of milk, fat, and protein in Australian Holstein-Friesians

2000 ◽  
Vol 51 (4) ◽  
pp. 515 ◽  
Author(s):  
M. R. Shariflou ◽  
C. Moran ◽  
F. W. Nicholas
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Bovine Growth Hormone ◽  
Data Sets ◽  
Production Traits ◽  
Milk Production Traits ◽  
Maximum Benefit ◽  
Pcr Products ◽  
Trait Locus

The occurrence of the Leu127/Val127 variants of the bovine growth hormone (bGH) gene and their effect on milk production traits was investigated in Australian Holstein-Friesian cattle. Animals were genotyped for the Leu127/Val127 variants, with RFLP methodology, using PCR and AluI digestion of PCR products (AluI-RFLP). Alleles Leu127 and Val127 occurred with frequencies of 82% and 18%, respectively. The quantitative effect of this polymorphic site on milk-production traits was estimated from lactation data and test-day data. Results from the 2 data sets consistently showed that the Leu127 allele is associated with higher production of milk, fat, and protein and is dominant to Val127. The average effects of the gene substitution are 95 L for milk yield, 7 kg for fat yield, and 3 kg for protein yield per lactation. This locus may be directly responsible for quantitative variation or it may be a marker for a closely linked quantitative trait locus (QTL) for milk-production traits in Australian dairy cattle. In either case, it will be useful as an aid to selection for improvement of milk production traits. As the Leu127 allele is dominant, selection of AI sires homozygous for the Leu127 allele (Leu127/Leu127) will result in maximum benefit without the need for genotyping cows.

scholarly journals L-selectin gene polymorphism and its association with clinical mastitis, somatic cell score, and milk production in Polish Holstein-Friesian cattle

2018 ◽  
Vol 63 (No. 7) ◽  
pp. 256-262 ◽  
Author(s):  
M. Dusza ◽  
J. Pokorska ◽  
J. Makulska ◽  
D. Kulaj ◽  
M. Cupial
Keyword(s):  
Somatic Cell ◽  
Milk Production ◽  
Milk Fat ◽  
Bacterial Infections ◽  
Clinical Mastitis ◽  
Somatic Cell Score ◽  
Production Traits ◽  
Milk Production Traits ◽  
Holstein Friesian ◽  
Friesian Cows

Bovine mastitis is a widespread disease of the mammary gland, highly contributing to the increase in veterinary costs in dairy industry. In the present study, the genetic polymorphism within bovine L-selectin gene was analysed and its impact on clinical mastitis occurrence, somatic cell score (SCS), and milk production traits in Polish Holstein-Friesian cows was examined. Polymorphism within L-selectin gene, molecule responsible for neutrophil attachment to endothelium, might have a potential role in immune response to bacterial infections and udder health. Two hundred and six Polish Holstein-Friesian cows were genotyped by polymerase chain reaction-restriction fragment length polymorphism method. Two single nucleotide polymorphisms mutations within the coding sequence of L-selectin gene were identified (c.165G&gt;A and c.567C&gt;T). The effect of c.165G&gt;A and c.567C&gt;T mutations on SCS was highly significant (P = 0.0019 and P = 0.0003, respectively). Strong associations (P ≤ 0.0001) were also observed between L-selectin polymorphism and milk production traits (milk yield, milk fat percentage, and milk protein percentage). However, the polymorphism in the analysed gene had no influence on the resistance or susceptibility of cows to clinical mastitis (only the tendency toward significance, P = 0.06 for c.567C&gt;T mutation was found). Potential exploitation of the information on the identified associations in genetic selection needs to confirm the obtained results in further investigations.

Growth hormone gene variability and its effects on milk traits in primiparous Sarda goats

2013 ◽  
Vol 80 (3) ◽  
pp. 255-262 ◽  
Author(s):  
Maria Luisa Dettori ◽  
Angela Maria Rocchigiani ◽  
Sebastiano Luridiana ◽  
Maria Consuelo Mura ◽  
Vincenzo Carcangiu ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Transcription Initiation ◽  
Growth Hormone Gene ◽  
Production Traits ◽  
Fat Percentage ◽  
Milk Traits ◽  
Exon 2 ◽  
Lactating Goats

The aim of this research was to investigate variability in each of the five exons of the caprine growth hormone (gGH) gene, in order to establish the possible relationships with milk traits in Sarda breed goat. The general linear model procedure was used to analyse the effects of the single strand conformation (SSCP) profiles on milk traits of 100 lactating goats. Analysis of conformational polymorphism at exons 1–5 revealed a total of 25 differing banding patterns. Sequencing revealed 21 nucleotide changes (compared with GenBank D00476): 14 were polymorphic and 7 monomorphic; 19 in exonic regions, 5 of which were nonsynonymous. A SNP upstream of the transcription initiation codon (c.-3A>G) and an indel (c.*29_30insC) in the 3′UTR, were detected. Alignment of 4 cloned sequences including the entire gGH gene led to the identification of 22 nucleotide variations within the intron regions, including two indels. Association analysis revealed that each exon, except exon-1, affected milk yield, exons 1 and 3 influenced milk fat percentage, and all exons, except exon-2, had an effect on protein percentage, supporting previous results in livestock. The variability detected at the caprine GH gene might provide useful information for the phylogeny of ruminants and, more importantly, have implications on the biological function of the growth hormone and on those traits resulting from its physiological action, including milk production and composition. The caprine GH gene may become a useful molecular marker for a more effective genetic selection for milk production traits in goats.

Associations of Growth Hormone Gene Polymorphisms with Milk Production Traits in South Anatolian and East Anatolian Red Cattle

2009 ◽  
Vol 8 (5) ◽  
pp. 1040-1044 ◽  
Author(s):  
Hasret Yardibi . ◽  
Gulhan Turkay Hostur . ◽  
Ipek Paya . ◽  
Ferhan Kaygisiz . ◽  
Gurhan Ciftioglu . ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Gene Polymorphisms ◽  
Growth Hormone Gene ◽  
Production Traits ◽  
Milk Production Traits

Effect of polymorphism in the 5’ promoter region of the TG gene on the milk production traits of Polish Holstein-Friesian cattle

2020 ◽  
Vol 16 (1) ◽  
pp. 9-16
Author(s):  
Ewa Czerniawska-Piątkowska ◽  
Inga Kowalewska-Łuczak
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Promoter Region ◽  
Point Mutations ◽  
Production Traits ◽  
Milk Production Traits ◽  
Gene Encoding ◽  
Holstein Friesian ◽  
Daily Milk Yield ◽  
The Relationship

The aim of the study was to determine the relationship between the occurrence of three different point mutations (257C>T, 335A>G and 422 C>T) in the promoter region of the gene encoding thyroglobulin (causing polymorphism) and the milk production traits of cattle. The research was conducted on a herd of Polish Holstein-Friesian cows. Genotypes were determined by PCR and its ACRS modification. The frequency of more common alleles for the point mutations was determined: C ‒ 0.830 (257C>T), A ‒ 0.765 (335A>G), and C ‒ 0.635 (422C>T). The statistical analysis showed that all three point mutations in the promoter region of the TG gene significantly (P ≤ 0.05; P ≤ 0.01) affect the analysed milk production traits, i.e. daily milk yield, content of milk fat and protein, and somatic cell count.

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