An Automated System for Maintaining Liquid Nitrogen Levels in the Gatan Cryostage.

2000 ◽  
Vol 6 (S2) ◽  
pp. 290-291
Author(s):  
S.J. Robinson ◽  
G. Fried ◽  
J. Pulokas
Keyword(s):  
Electron Microscope ◽  
Liquid Nitrogen ◽  
Transmission Electron Microscope ◽  
Ccd Camera ◽  
Automated System ◽  
Automated Control ◽  
Nitrogen Levels ◽  
Transmission Electron ◽  
Current Implementation

For several years we have been developing a system for the automated control and acquisition of images from a transmission electron microscope [1,2,3]. The system has been developed around a Philips CM200 equipped with a Gatan cryostage and a Gatan MSC CCD camera. One of the primary motivations in developing this software is to provide for a system that can acquire many hundreds of images over several days of completely unattended operation.The current implementation of our automated system allows data to be automatically collected from the TEM for more than 36 hours. There is no intervention required from the operator except for one critical exception, refilling the Gatan cryostage. The cryostage, which maintains the temperature of the vitreous ice specimens, must be refilled with liquid nitrogen (LN2) approximately every two hours.

Investigations of Fine Grained Metallic Materials by Means of Orientation Maps in Transmission Electron Microscope

2012 ◽  
Vol 186 ◽  
pp. 53-57 ◽  
Author(s):  
Magdalena Bieda
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Quantitative Description ◽  
Ccd Camera ◽  
Microscopic Investigation ◽  
Automated System ◽  
Fine Grained ◽  
Transmission Electron ◽  
Orientation Maps ◽  
Diffraction Patterns

New subdivision of microscopic investigation called Orientation Microscopy (OM) is already well known in scanning electron microscope (SEM). Needs for investigation in nanoscale contribute to development of an appropriate method for transmission electron microscope (TEM). Automated acquisition and indexing of diffraction patterns, necessary for creation of orientation maps in TEM, cause more difficulties then in SEM. Nevertheless, the techniques of OM are also being developed in the Transmission Electron Microscope (TEM). Microdiffraction has been successfully introduced for creating such maps. Individual orientation measurements, which appeared in the convergent beam mode, can be used for quantitative description of microstructure of fine grained and deformed materials. The idea of the operation of the automated system in transmission electron microscope (TEM) which is developed in IMIM PAS relies on an automatic (with control position of the beam) acquisition of diffraction patterns using digital CCD camera, and indexing them, and then on the analysis of the set of individual crystallographic orientations. The graphic presentation of received sets of orientation can be analysed in order to obtain parameters and characteristics such as texture characteristics, characteristics of grain boundaries (based on orientation relationship) or the stereological characteristics. To illustrate application of this system, orientation maps measured in cold-rolled polycrystalline aluminium and its alloy 6013, and in multi-phase alloys of Fe-Cr-Co system after severe plastic deformation are presented.

An Interactive User Interface for Automated Acquisition of Transmission Electron Micrographs

2000 ◽  
Vol 6 (S2) ◽  
pp. 288-289
Author(s):  
J. Pulokas ◽  
N. Kisseberth ◽  
C.S. Potter ◽  
B. Carragher
Keyword(s):  
Electron Microscope ◽  
User Interface ◽  
Low Dose ◽  
Ccd Camera ◽  
Automated Control ◽  
Software Application ◽  
Transmission Electron ◽  
Set Up ◽  
Interactive User ◽  
Interactive User Interface

For several years we have been developing a software application, called Leginon, for the automated control and acquisition of images from a transmission electron microscope. The system has been developed around a Philips CM200 and a Gatan MSC CCD camera. One of the primary motivations in developing this software is to provide for a system that can acquire many hundreds of images under low dose conditions from a specimen embedded in vitreous ice. In order to set up and manage this system we have developed a number of simple interactive graphical tools that enable the user to design, oversee and manage protocols for controlling the microscope and collecting the images. Many of these simple tools have also proved generally useful as stand alone applications.

Structure and symmetry of CaFeTi2O6 perovskite

1995 ◽  
Vol 53 ◽  
pp. 364-365
Author(s):  
Nan Yao ◽  
Alexandra Navrotsky ◽  
Kurt Leinenweber
Keyword(s):  
Electron Microscope ◽  
Liquid Nitrogen ◽  
Transmission Electron Microscope ◽  
Point Group ◽  
Carbon Film ◽  
Group Symmetry ◽  
Point Group Symmetry ◽  
Specimen Holder ◽  
Transmission Electron ◽  
Calcium Iron

A new calcium iron (II) titanate ordered perovskite (CaFeTi2O6) was recently synthesized from an equimolar mixture of CaTiO3 and FeTiO3 at 12-15 GPa and 1200-1400 °C. In the present paper, we discuss the structure and symmetry studies of this new compound CaFeTi2O6 using CBED and HREM techniques. The CaFeTi2O6 powder sample was crushed to small fragments with an agate mortar and pestle under purified methanol. A drop of the resulting suspension was placed on a copper grid coated with holey-carbon film. CBED and HREM studies were performed on a Philips-CM20 ST transmission electron microscope equipped with a double tilt, liquid-nitrogen-cooled specimen holder under moderate vacuum conditions over the range from 10-6-10-7 Torr. CBED makes it possible to examine the diffraction symmetry of key orientations of the crystal and therefore determine the point-group symmetry of the crystal. This information, along with the dynamic extinction information on systematic absences, can be used to determine the crystallographic space group uniquely.

Preparation of Cross-Sectional TEM Samples of Fe-Zn Couples

1991 ◽  
Vol 254 ◽  
Author(s):  
L. A. Giannuzzi ◽  
P. R. Howell ◽  
H. W. Pickering ◽  
W. R. Bidter
Keyword(s):  
Electron Microscope ◽  
Liquid Nitrogen ◽  
Cross Section ◽  
Transmission Electron Microscope ◽  
Preparation Technique ◽  
Ion Milling ◽  
Tem Analysis ◽  
Cross Sectional ◽  
Cold Stage ◽  
Transmission Electron

AbstractA preparation technique for the production of cross-sectional transmission electron microscope (TEM) samples from the interdiffusion regions of Fe-Zn binary couples is described. To alleviate the problem of unequal ion milling rates between the Fe and Zn, a 0.75mm thick Fe sheet has been double plated with a thick electrodeposited Zn coating to achieve a total couple thickness of ˜3mm. After slicing the couple in cross-section, the Fe region of the sample is dimpled to perforation near the Fe-Zn interface. Final thinning for TEM analysis is obtained by ion milling using a liquid nitrogen cold stage and sector speed control. The ion milling procedure is stopped when the perforated hole in the Fe-side of the couple extends through the faster eroding Zn-side of the interface. This technique, in modified form, is expected to be suitable for commercial steels coated with Zn-based alloys.

Database of Electron Microscope Images on the World Wide Web

1997 ◽  
Vol 3 (S2) ◽  
pp. 1105-1106
Author(s):  
A. Taniyama ◽  
D. Shindo ◽  
K. Hiraga ◽  
T. Oikawa ◽  
M. Kersker
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
World Wide ◽  
Computer Network ◽  
Ccd Camera ◽  
Digital Data ◽  
Imaging Plate ◽  
Network Systems ◽  
Transmission Electron ◽  
Microscope Images

Recently, new recording devices, such as the Imaging Plate and a slow scan CCD camera, have come into wide use for digitally recording and storing transmission electron microscope images. A digital image has advantages of transferring, sharing and storing data. Furthermore the improvement of world wide computer network systems, for example, Internet makes it easier to transfer and share digital data among researchers. We have studied the processing and a quantitative analysis of digital high-resolution transmission electron microscope (HREM) images by means of a standard EWS and an SX-3 super-computer on the intelligent computer network system in Tohoku University, which is called Super TAINS (Fig. 1). With the increase of digital HREM images, we are in need of tools to help us store and quickly search these data.A web site called “EMILIA (Electron Microscope Image Library and Archive)” was established in October 1996 as a database of HREM images. EMILIA is implemented by a WWW server on Super TAINS and HTML documents. Figure 2 shows sample pages from EMILIA.

Energy-filtered chemical mapping: Current applications to materials science

1994 ◽  
Vol 52 ◽  
pp. 958-959
Author(s):  
P. A. Crozier
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Materials Science ◽  
Ccd Camera ◽  
Image Plane ◽  
Scanning Transmission Electron Microscope ◽  
Thermal Source ◽  
Chemical Mapping ◽  
Elemental Mapping ◽  
Transmission Electron

Elemental mapping is a powerful technique for elucidating the distribution of elements on the nanometer scale in materials with complex morphologies. In electron microscopy, mapping is usually performed on a scanning transmission electron microscope (STEM) fitted with a field emission gun (FEG) using the techniques of energy-dispersive x-ray spectroscopy (EDX) or electron energy-loss spectroscopy (EELS). However, recent advances in spectrometer design and digital image recording have stimulated renewed interest in energy-filtered imaging and elemental mapping with a conventional transmission electron microscope. Here, some applications of energy-filtered chemical mapping in materials science are described.Experiments were conducted on a Zeiss 912 operating at 120 kV with a thermal source and equipped with an omega filter spectrometer. A variable width slit in the spectrometer image plane allows energy-filtered images to be formed on the microscope viewing screen. These images are digitally recorded using a Gatan 679 slow-scan CCD camera with 1024 pixels.

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

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