scholarly journals Insights into the Mechanisms of Screw Feeder Plugging by Heated Pine Forestry Residues Using In-situ and Correlative Microscopy

2020 ◽  
Vol 26 (S2) ◽  
pp. 2778-2780
Author(s):  
Josephine Gruber ◽  
Yining Zeng ◽  
Steven Rowland ◽  
Daniel Carpenter ◽  
Bryon Donohoe
Keyword(s):  
Correlative Microscopy ◽  
Screw Feeder ◽  
Forestry Residues

scholarly journals Lifestyle of sponge symbiont phages by host prediction and correlative microscopy

2021 ◽  
Author(s):  
M. T. Jahn ◽  
T. Lachnit ◽  
S. M. Markert ◽  
C. Stigloher ◽  
L. Pita ◽  
...  
Keyword(s):  
Distinct Pattern ◽  
Microbial Consortia ◽  
Bacterial Symbionts ◽  
Correlative Microscopy ◽  
Phage Ecology ◽  
Infection Dynamics ◽  
Imaging Approach ◽  
Animal Hosts ◽  
Viral Distribution

AbstractBacteriophages (phages) are ubiquitous elements in nature, but their ecology and role in animals remains little understood. Sponges represent the oldest known extant animal-microbe symbiosis and are associated with dense and diverse microbial consortia. Here we investigate the tripartite interaction between phages, bacterial symbionts, and the sponge host. We combined imaging and bioinformatics to tackle important questions on who the phage hosts are and what the replication mode and spatial distribution within the animal is. This approach led to the discovery of distinct phage-microbe infection networks in sponge versus seawater microbiomes. A new correlative in situ imaging approach (‘PhageFISH-CLEM‘) localised phages within bacterial symbiont cells, but also within phagocytotically active sponge cells. We postulate that the phagocytosis of free virions by sponge cells modulates phage-bacteria ratios and ultimately controls infection dynamics. Prediction of phage replication strategies indicated a distinct pattern, where lysogeny dominates the sponge microbiome, likely fostered by sponge host-mediated virion clearance, while lysis dominates in seawater. Collectively, this work provides new insights into phage ecology within sponges, highlighting the importance of tripartite animal-phage-bacterium interplay in holobiont functioning. We anticipate that our imaging approach will be instrumental to further understanding of viral distribution and cellular association in animal hosts.

scholarly journals Multimodal correlative microscopy for in situ detection and quantification of chemical elements in biological specimens. Applications to nanotoxicology

2015 ◽  
Vol 8 (4) ◽  
pp. 159-167
Author(s):  
Quentin Le Trequesser ◽  
Gladys Saez ◽  
Marina Simon ◽  
Guillaume Devès ◽  
Laurent Daudin ◽  
...  
Keyword(s):  
Chemical Elements ◽  
Correlative Microscopy ◽  
In Situ Detection ◽  
Detection And Quantification

scholarly journals PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Sergey Gorelick ◽  
Genevieve Buckley ◽  
Gediminas Gervinskas ◽  
Travis K Johnson ◽  
Ava Handley ◽  
...  
Keyword(s):  
Focused Ion Beam ◽  
Fluorescent Proteins ◽  
Electron Tomography ◽  
Protein Complexes ◽  
Ion Beam ◽  
Correlative Microscopy ◽  
New Approach ◽  
Macromolecular Complexes ◽  
Cryo Electron Tomography

Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella preparation through cryo-focused ion beam (FIB) milling and correlative light microscopy to ensure that the event of interest is present in the lamella. Here, we present an integrated cryo-FIB and light microscope setup called the Photon Ion Electron microscope (PIE-scope) that enables direct and rapid isolation of cellular regions containing protein complexes of interest. Specifically, we demonstrate the versatility of PIE-scope by preparing targeted cryo-lamellae from subcellular compartments of neurons from transgenic Caenorhabditis elegans and Drosophila melanogaster expressing fluorescent proteins. We designed PIE-scope to enable retrofitting of existing microscopes, which will increase the throughput and accuracy on projects requiring correlative microscopy to target protein complexes. This new approach will make cryo-correlative workflow safer and more accessible.

scholarly journals Correlative Microscopy of Vitreous Sections Provides Insights into BAR-Domain Organization In Situ

Structure ◽  
2018 ◽  
Vol 26 (6) ◽  
pp. 879-886.e3 ◽  
Author(s):  
Tanmay A.M. Bharat ◽  
Patrick C. Hoffmann ◽  
Wanda Kukulski
Keyword(s):  
Correlative Microscopy ◽  
Domain Organization ◽  
Bar Domain

scholarly journals Combining SEM with AFM for in situ Correlative Microscopy

2016 ◽  
pp. 999-1000
Author(s):  
Stephan Kleindiek ◽  
Massoud Dadras ◽  
Klaus Schock ◽  
Andreas Lieb ◽  
Gregor Renka
Keyword(s):  
Correlative Microscopy
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