Screening of wild and cultivated lentil germplasm for resistance to stemphylium blight

Lens culinaris Medik. ssp. culinaris is the only cultivated species in the genus Lens. Intensive selection pressure to develop new cultivars, a narrow genetic base, co-evolution of pathogens to partially resistant cultivars and other factors have accelerated susceptibility to different fungal diseases in this species. Few sources for resistance to stemphylium blight (SB) caused by Stemphylium botryosum Wallr. exist among commercial lentil cultivars. A total of 70 accessions were selected from seven species of the genus Lens to screen for SB resistance. The L. culinaris accessions were screened in four different environments, and the accessions of Lens ervoides, L. culinaris ssp. orientalis, Lens tomentosus,Lens nigricans, Lens odemensis and Lens lamottei in growth chamber or greenhouse experiments to identify resistance sources for potential use in lentil breeding. A highly aggressive isolate of SB was used as an inoculum to screen them under controlled conditions. Lentil cultivars ‘Eston’ (resistant) and ‘CDC Glamis’ (susceptible) were used as checks with consistent results in all experiments. Most of the L. culinaris accessions were susceptible to SB, whereas more than 70% of the wild lentil accessions had disease severity scores equal to or significantly lower than that of the SB-resistant check ‘Eston’. Some wild species accessions previously identified with resistance to anthracnose (Colletotrichum truncatum) and ascochyta blight (Ascochyta lentis) were also highly resistant to SB. The highest frequency of resistance to SB was found in L. lamottei followed by L. ervoides of the secondary gene pool. These sources can potentially be used to develop new commercial cultivars with multiple or single disease resistance.

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Evaluation of resistance to stemphylium blight in interspecific recombinant inbred lines derived from Lens culinaris × Lens ervoides

Plant Genetic Resources ◽

10.1017/s1479262120000295 ◽

2020 ◽

Vol 18 (4) ◽

pp. 251-258

Author(s):

Stanley Adobor ◽

Rajib Podder ◽

Sabine Banniza ◽

Albert Vandenberg

Keyword(s):

Disease Severity ◽

Recombinant Inbred ◽

Lens Culinaris ◽

Research Centre ◽

Blight Resistance ◽

Resistant Parent ◽

Controlled Conditions ◽

Severity Scores ◽

Ril Population ◽

Stemphylium Blight

AbstractCultivated lentil suffers yield loss from stemphylium blight, caused by Stemphylium botryosum Wallr. Identification of sources of stemphylium blight resistance and knowledge of the mode of inheritance of resistance are important for developing resistant cultivars. The interspecific recombinant inbred line (RIL) population developed from a cross between the moderately resistant parent Lens culinaris cv. ‘Eston’ and the resistant parent L. ervoides (Brign.) Grande accession IG 72815 was evaluated for stemphylium blight resistance under controlled conditions at the University of Saskatchewan, Saskatoon, Canada, and under field conditions at the Pulses Research Centre (PRC), Ishurdi, Bangladesh. We hypothesized that resistance from both parents will lead to transgressive segregation indicative of pyramiding of resistance genes from the same. However, no resistant transgressive segregants were observed in the RIL population. A large proportion (50%) of the RILs had disease severity levels similar to the resistant parent IG 72815 in experiments conducted under natural disease pressure in Bangladesh. Under controlled conditions in Saskatoon, 38% of RILs had resistance levels similar to IG 72815. Across all environments, 14 RILs consistently had resistance levels similar to IG 72815. The distribution of disease severity scores for all RILs indicated polygenic inheritance of stemphylium blight resistance in the population. RILs with consistent resistant reactions should prove useful for lentil improvement programmes. This will contribute to increasing the productivity of lentil crops in North America and the Indo-Gangetic region, which account for more than 68% of world lentil production.

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Lentisone, a New Phytotoxic Anthraquinone Produced by Ascochyta lentis, the Causal Agent of Ascochyta Blight in Lens culinaris

Journal of Agricultural and Food Chemistry ◽

10.1021/jf4026663 ◽

2013 ◽

Vol 61 (30) ◽

pp. 7301-7308 ◽

Cited By ~ 16

Author(s):

Anna Andolfi ◽

Alessio Cimmino ◽

Angel M. Villegas-Fernández ◽

Angela Tuzi ◽

Antonello Santini ◽

...

Keyword(s):

Lens Culinaris ◽

Ascochyta Blight ◽

Causal Agent ◽

Ascochyta Lentis

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Defense responses of lentil (Lens culinaris) genotypes carrying non-allelic ascochyta blight resistance genes to Ascochyta lentis infection

PLoS ONE ◽

10.1371/journal.pone.0204124 ◽

2018 ◽

Vol 13 (9) ◽

pp. e0204124 ◽

Cited By ~ 7

Author(s):

Ehsan Sari ◽

Vijai Bhadauria ◽

Larissa Ramsay ◽

M. Hossein Borhan ◽

Judith Lichtenzveig ◽

...

Keyword(s):

Resistance Genes ◽

Lens Culinaris ◽

Ascochyta Blight ◽

Defense Responses ◽

Blight Resistance ◽

Ascochyta Lentis

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First Report of Lentil Ascochyta Blight Caused by Ascochyta lentis in Italy

Plant Disease ◽

10.1094/pdis.1999.83.1.77c ◽

1999 ◽

Vol 83 (1) ◽

pp. 77-77

Author(s):

C. Cappelli ◽

R. Buonaurio ◽

R. Torricelli

Keyword(s):

Lens Culinaris ◽

Ascochyta Blight ◽

Central Italy ◽

Morphological Characteristics ◽

Potato Dextrose Agar ◽

Conidial Suspension ◽

First Report ◽

Ascochyta Lentis ◽

Humidity Chamber ◽

Dry Conditions

In May 1997, ascochyta blight incited by Ascochyta lentis Vassiljevsky was observed at an incidence of less than 5% in lentil (Lens culinaris Medik.) fields in Umbria (Central Italy). Symptoms appeared on leaves and stems as tan spots surrounded by a dark margin. Small black pycnidia that produced a pink exudate containing hyaline, 1 septate, 14.2 to 15.8 × 3.5 μm conidia under high humidity were visible in the center of the spots. The fungus was consistently isolated on potato dextrose agar from diseased leaves or stems. To satisfy Koch's postulates, a conidial suspension (106 conidia per ml) of the fungus was sprayed on leaves of 20-day-old lentil plants (landrace Castelluccio) that were maintained in a humidity chamber for 96 h after inoculation. Lesions resembling symptoms that occurred in the field were observed on plants 3 weeks after inoculation. Symptoms were not observed on control plants sprayed with water. The fungus reisolated from the diseased plants was identical to the original isolates. Based on morphological characteristics of pycnidia and conidia as well as pathogenicity, the fungus was identified as A. lentis. A deep-freeze blotter method (2) was used to detect A. lentis in lentil seeds of 20 local landraces used by Umbrian farmers and two accessions from Canada and Turkey, as well as in seed collected from infected fields. The fungus was present only in the two lentil accessions with an incidence of about 5%. Although the fungus had been isolated from Italian seed germplasm in 1986 (1), this is the first report of ascochyta blight occurring in lentil crops in Italy. The heavy rainfalls that characterize the first stage of lentil cultivation in Umbria are favorable for disease development while hot and dry conditions that usually occur during flowering and maturation prevent the dissemination of inoculum and the infection of the seeds. For these reasons, some Umbrian areas could be more suitable for production of ascochyta-free lentil seeds. References: (1) W. J. Kaiser and R. M. Hannan. Phytopathology 76:355, 1986. (2) T. Limonard. Proc. Int. Seed Test. Assoc. 33:343, 1968.

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CDC Milestone lentil

Canadian Journal of Plant Science ◽

10.4141/p00-043 ◽

2001 ◽

Vol 81 (1) ◽

pp. 113-114 ◽

Cited By ~ 11

Author(s):

A. Vandenberg ◽

F. A. Kiehn ◽

C. Vera ◽

R. Gaudiel ◽

L. Buchwaldt ◽

...

Keyword(s):

Lens Culinaris ◽

Ascochyta Blight ◽

Western Canada ◽

Crop Development ◽

Ascochyta Lentis ◽

Cultivar Description ◽

Canadian Food Inspection Agency ◽

Production Areas ◽

Pale Green ◽

Yield Trials

CDC Milestone is a high-yielding, yellow cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre, Saskatoon, Saskatchewan. It is intended for cultivation in all lentil production areas of western Canada. CDC Milestone was evaluated for yield, resistance to ascochyta blight (Ascochyta lentis Vassilievsky) and agronomic performance as breeding line 512-2 in the Lentil Co-operative Yield Trials in 1995 and 1996. It has small seeds with pale green seed coat and is acceptable in the small green market class of lentil. CDC Milestone was granted official registration (#4836) as a cultivar in Canada by the Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency on 8 December 1998. Key words: Lentil, cultivar description, Lens culinaris

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Two major genes confer Ascochyta blight resistance in Lens orientalis

New Zealand Plant Protection ◽

10.30843/nzpp.2000.53.3622 ◽

2000 ◽

Vol 53 ◽

pp. 109-113 ◽

Cited By ~ 2

Author(s):

G. Ye ◽

D.L. McNeil ◽

G.D. Hill

Keyword(s):

Lens Culinaris ◽

Genetic Model ◽

Ascochyta Blight ◽

Dominant Gene ◽

Blight Resistance ◽

Published Data ◽

Mechanisms Of Resistance ◽

Genetic Mechanisms ◽

Wild Lentil ◽

Better Than

Genetic mechanisms of resistance to Ascochyta blight from wild lentil (Lens orientalis) were studied using F3 families of a cross between the cultivar Titore of the cultivated lentil (Lens culinaris) and accession W 6 3261 of the wild lentil The simplest genetic model to adequately fit the data involved two genes one partially dominant gene with a larger effect (A) and one dominant gene with a less effect (B) which acted additively to confer resistance One copy of the resistance increasing allele of the A gene is not sufficient for a genotype to be resistant nor is one or two copies of the resistance increasing allele of the B gene alone When fitted to other published data this model was as good as or better than alternative models that have been proposed

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QTL mapping of resistance in lentil (Lens culinaris ssp. culinaris) to ascochyta blight (Ascochyta lentis)

Plant Breeding ◽

10.1111/j.1439-0523.2006.01259.x ◽

2006 ◽

Vol 125 (5) ◽

pp. 506-512 ◽

Cited By ~ 43

Author(s):

P. W. J. Taylor ◽

P. K. Ades ◽

R. Ford

Keyword(s):

Qtl Mapping ◽

Lens Culinaris ◽

Ascochyta Blight ◽

Ascochyta Lentis

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Biochemical Basis of Resistance in Lentil (Lens culinaris Medik.) Against Ascochyta Blight: I Phenols

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2000.1141.1143 ◽

2000 ◽

Vol 3 (7) ◽

pp. 1141-1143 ◽

Cited By ~ 5

Author(s):

S.T. Sahi ◽

M. A. Randhawa . ◽

Nighat Sarwar . ◽

S.M. Khan .

Keyword(s):

Lens Culinaris ◽

Ascochyta Blight ◽

Biochemical Basis

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Verification of disease management technology on lentil against Stemphylium blight at farmer’s field in Nepal

Journal of Agriculture and Natural Resources ◽

10.3126/janr.v1i1.22222 ◽

2018 ◽

Vol 1 (1) ◽

pp. 51-61

Author(s):

Subash Subedi ◽

Sarswati Neupane

Keyword(s):

Lens Culinaris ◽

Block Design ◽

Cost Ratio ◽

Row Spacing ◽

Benefit Cost Ratio ◽

Plot Size ◽

Management Technology ◽

Randomized Complete Block Design ◽

Stemphylium Blight ◽

Benefit Cost

Technologies generated from lentil (Lens culinaris Medik) stemphylium blight (Stemphylium botryosum Walr) management experiments were verified at farmers field of 5 districts viz., Chitawan, Rautahat, Dang, Parsa and Banke during two winter seasons of 2013-2014 and 2014- 2015. The experiment was laid out in randomized complete block design with factorial arrangement of treatments and replicated 4 times. The plot size was 340 m2 (1 Kattha) with 25 cm row to row spacing. There were altogether 3 factors of the experiment i.e. year (2013-2014 and 2014-2015), location (5 districts) and package of practice (improved and farmers practice). The higher crop yield (1142.50 kg/ha) with lower disease index (34.95%) and higher benefit cost ratio of 2.42 were recorded in the farmers field of Banke district following seed rate (30 kg/ha), 8 hour primed improved variety (Black lentil), fertilizer doze of (20:40:20 NPK kg/ha+ 1 kg/ha B basal doze) and subsequent 3 sprays of Dithane M-45 @ 2.5 g/l of water at 10 days interval.

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