Molecular characterization of oil palmElaeis guineensisJacq. materials from Cameroon

2013 ◽  
Vol 11 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Diana Arias ◽  
Carmenza Montoya ◽  
Hernán Romero
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Molecular Characterization ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Low Frequency ◽  
Molecular Data ◽  
Ex Situ ◽  
Geographical Regions

The narrow genetic base of existing commercial oil palm cultivars has prompted oil palm breeders to give increased importance to augmenting these genetic resources because the sustainable development of the crop depends largely on the availability of genetic diversity and its use. Therefore, the purpose of this study was to conduct a molecular characterization of anex situcollection of oil palmElaeis guineensisJacq. populations from the Republic of Cameroon using microsatellite molecular markers. Overall, 31 simple sequence repeats were polymorphic, with a total of 223 alleles, 78.4% of which were found at low frequency. The total genetic diversity was relatively high (HT= 0.673). The genetic differentiation between geographical regions was low (GST= 0.023,P= 0.001), and between families it was high (GST= 0.166,P= 0.001), showing greater variation between families than among geographical regions. The molecular data indicate that genetic diversity among the genotypes evaluated is mainly distributed within regions, suggesting that there is no isolation by geographical distance and that all the sampled individuals form a single diverse population. Therefore, it was concluded that a relatively low number of accessions (120 in the analysed case) that includes at least one representative of each family would allow us to efficiently collect almost the entire genetic diversity of Cameroon within the collection studied. This will allow for the efficient use of genetic resources and a reduction in morpho-agronomic characterization costs.

scholarly journals Assessment of genetic diversity and population structure of oil palm (Elaeis guineensis Jacq.) field genebank: A step towards molecular-assisted germplasm conservation

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0255418
Author(s):  
Siou Ting Gan ◽  
Chin Jit Teo ◽  
Shobana Manirasa ◽  
Wei Chee Wong ◽  
Choo Kien Wong
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Oil Palm ◽  
Core Collection ◽  
Elaeis Guineensis ◽  
Germplasm Conservation ◽  
Ex Situ ◽  
Fixation Index ◽  
Core Set ◽  
Genetic Diversity And Structure

Oil palm (Elaeis guineensis) germplasm is exclusively maintained as ex situ living collections in the field for genetic conservation and evaluation. However, this is not for long term and the maintenance of field genebanks is expensive and challenging. Large area of land is required and the germplasms are exposed to extreme weather conditions and casualty from pests and diseases. By using 107 SSR markers, this study aimed to examine the genetic diversity and relatedness of 186 palms from a Nigerian-based oil palm germplasm and to identify core collection for conservation. On average, 8.67 alleles per SSR locus were scored with average effective number of alleles per population ranging from 1.96 to 3.34 and private alleles were detected in all populations. Mean expected heterozygosity was 0.576 ranging from 0.437 to 0.661 and the Wright’s fixation index calculated was -0.110. Overall moderate genetic differentiation among populations was detected (mean pairwise population FST = 0.120, gene flow Nm = 1.117 and Nei’s genetic distance = 0.466) and this was further confirmed by AMOVA analysis. UPGMA dendogram and Bayesian structure analysis concomitantly clustered the 12 populations into eight genetic groups. The best core collection assembled by Core Hunter ver. 3.2.1 consisted of 58 palms accounting for 31.2% of the original population, which was a smaller core set than using PowerCore 1.0. This core set attained perfect allelic coverage with good representation, high genetic distance between entries, and maintained genetic diversity and structure of the germplasm. This study reported the first molecular characterization and validation of core collections for oil palm field genebank. The established core collection via molecular approach, which captures maximum genetic diversity with minimum redundancy, would allow effective use of genetic resources for introgression and for sustainable oil palm germplasm conservation. The way forward to efficiently conserve the field genebanks into next generation without losing their diversity was further discussed.

Genetic diversity and establishment of a core collection of oil palm (Elaeis guineensisJacq.) based on molecular data

2014 ◽  
Vol 13 (3) ◽  
pp. 256-265 ◽  
Author(s):  
Diana Arias ◽  
Maria González ◽  
Hernán Romero
Keyword(s):  
Genetic Diversity ◽  
Genetic Differentiation ◽  
Genetic Resources ◽  
Oil Palm ◽  
Core Collection ◽  
Molecular Data ◽  
Core Collections ◽  
Mini Core Collection ◽  
Entire Collection ◽  
Breeding Programmes

Understanding of genetic diversity and its distribution is essential for promoting the use of genetic resources. The development of core collections using molecular tools has been proposed as a strategy for increasing the economical use and conservation of genetic resources. In this study, we investigated the genetic variation among different geographical origins and potential entries that constituted a core collection of oil palm, using 29 microsatellite markers and by evaluating 788 oil palm accessions. Our results revealed important genetic diversity (HT= 0.759) between oil palm accessions from Angola and Cameroon, which exhibited a low coefficient of genetic differentiation between populations (GST= 0.022). However, the inclusion of oil palm accessions from Indonesia in the analysis resulted in a high coefficient of genetic differentiation between populations (GST= 0.251). We found that the combination of stratified sampling based on a sorting method and a heuristic algorithm was the most effective method for the development of an oil palm core collection set. Using this method, two core collections were identified. The first core collection, comprising 289 entries, contained 271 retained alleles in a sample representing 37% of the entire collection. The second one is a mini core collection, comprising 91 entries, that contained 271 retained alleles with a totalHevalue of 0.72 in a sample representing 11% of the entire collection. The information reported in this study will be of great interest to oil palm researchers because new strategies for breeding programmes can be developed based on these advances.

scholarly journals Molecular characterization of Bulgarian livestock genetic resources: 1. Genetic diversity in Bulgarian grey cattle as revealed by microsatellite markers

2005 ◽  
Vol 21 (5-6-2) ◽  
pp. 35-42 ◽  
Author(s):  
Atanaska Teneva ◽  
Elena Todorovska ◽  
Nikolai Tyufekchiev ◽  
Lasar Kozelov ◽  
Atanas Atanassov ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Resources ◽  
Molecular Characterization

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scholarly journals Intelligent Characterization of Lentil Genetic Resources: Evolutionary History, Genetic Diversity of Germplasm, and the Need for Well‐Represented Collections

2021 ◽  
Vol 1 (5) ◽  
Author(s):  
Azalea Guerra‐García ◽  
Tania Gioia ◽  
Eric Wettberg ◽  
Giuseppina Logozzo ◽  
Roberto Papa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Evolutionary History

scholarly journals Genetic diversity in oil palm (Elaeis guineensis Jacq) using RAM (Random Amplified Microsatellites)

Bragantia ◽  
2018 ◽  
Vol 77 (4) ◽  
pp. 546-556
Author(s):  
Christian Camilo Castañeda Cardona ◽  
Yacenia Morillo Coronado ◽  
Ana Cruz Morillo Conronado ◽  
Iván Ochoa
Keyword(s):  
Genetic Diversity ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

Molecular characterization of echovirus 13 uncovering high genetic diversity and identification of new genotypes in Pakistan

2017 ◽  
Vol 48 ◽  
pp. 102-108
Author(s):  
Shahzad Shaukat ◽  
Mehar Angez ◽  
Tariq Mahmood ◽  
Muhammad Masroor Alam ◽  
Salmaan Sharif ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
High Genetic Diversity

scholarly journals Physicochemical characterization of bunches from American oil palm (Elaeis oleifera H.B.K. Cortes) and their hybrids with African oil palm (Elaeis guineensis Jacq.)

2018 ◽  
Vol 67 (1) ◽  
pp. 170-178 ◽  
Author(s):  
Gabriel Chaves ◽  
Gustavo Adolfo Ligarreto- Moreno ◽  
Daniel Gerardo Cayon-Salinas
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Physicochemical Characterization ◽  
Elaeis Oleifera ◽  
Elaeis Guineensis Jacq ◽  
African Oil Palm

El objetivo de este estudio fue realizar un análisis comparativo de las características físicas y químicas de racimos de genotipos de Elaeis oleifera y de sus híbridos interespecíficos OxG con Elaeis guineensis, determinando los componentes y el potencial del aceite del racimo, y la calidad de los aceites, analizando el contenido de ácidos grasos, vitamina E y carotenos. En el estudio se utilizaron racimos provenientes de inflorescencias sin polinización asistida con la presencia perimetral de E. guineensis. Se utilizó un diseño experimental completamente al azar con tres unidades experimentales, cada una conformada por tres racimos. Los mayores cuajados del fruto se encontraron en el genotipo de E. oleifera Sinú (76,53 %) y el híbrido OxG II (72,64 %). Los potenciales de extracción de aceite fueron superiores en los materiales híbridos OxG destacándose el II (20,82 %). Las palmas E. oleifera presentaron mejores perfiles de ácidos grasos, destacándose los materiales del genotipo Sinú (79,1 % de ácidos grasos insaturados) y los del híbrido II (70,2 %). Para el contenido de vitamina E se confirmó la alta calidad del aceite de los materiales de E. oleifera, sobresaliendo el genotipo Coarí (1.006,7 ppm) y el híbrido II (1.549,6 ppm); el material del genotipo Sinú registró el mayor contenido de carotenos totales (1.524,7 ppm).

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