Intelligent Characterization of Lentil Genetic Resources: Evolutionary History, Genetic Diversity of Germplasm, and the Need for Well‐Represented Collections

AbstractMicrosatellites were used as a very effective tool for genetic diversity analysis and characterization of 51 grapevine (Vitis vinifera L.) accessions from the national collection of genetic resources. Genetic diversity was relatively high, 8.91 alleles were detected per analysed microsatellite locus in average, and fifty-one accessions were distinguished into 45 groups. Distribution of recent Slovak cultivars across the dendrogram accented both their genetic diversity and the effectiveness of the national breeding program in maintaining genetic diversity and generating new genetic variants. Each cultivar was different from the others and twelve of them contained 77.6% of the total genetic diversity of the whole analysed set. Microsatellite patterns were also able to confirm parentage in selected Slovak cultivars. An unusual phenomenon of triallelism was also detected in one of the analysed accessions. The present study has initiated molecular characterization within the national grapevine genetic resource collection and their comparison with well-established international cultivars.

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Characterization of the Genetic Resources of Farmed Tambaqui in Northern Brazil

Journal of Agricultural Science ◽

10.5539/jas.v9n10p76 ◽

2017 ◽

Vol 9 (10) ◽

pp. 76 ◽

Cited By ~ 1

Author(s):

Paola F. Fazzi-Gomes ◽

Nuno F. Melo ◽

Glauber Palheta ◽

Jonas Aguiar ◽

Iracilda Sampaio ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Genetic Resources ◽

Allelic Richness ◽

Diversity Indices ◽

Significant Loss ◽

Effective Strategies ◽

Northern Brazil

The present study analyzed the genetic variability and structure of farmed tambaqui in the Brazilian state of Pará, and provided basic information that can be used for the development of programs of monitoring and management of genetic resources in the aquaculture operations of northern Brazil. A total of 216 individuals were sampled from tambaqui farms in Pará. Genotyping was based on a multiplex set of 10 tri- and tetra-nucleotide microsatellite markers. The data were used to calculate genetic diversity indices, expected and observed heterozygosity, the number of alleles per locus, allelic richness, and inbreeding coefficient. Genetic structure was verified using DEST and RST, the genetic signature, and Bayesian analysis. The results showed that the tambaqui farms surveyed have suffered a significant loss of genetic variability, and that they are genetically structured, forming two clusters, one encompassing the farms in western Pará, and the other including the farms from the northeast and southeast regions of the state. These finding provide fundamental insights for the development of effective strategies that will help guarantee productivity and the quality of the tambaqui farms of northern Brazil, and provide a database for the upgrading of the genetic variability of these populations. This study indicated the need for hatcheries in southeastern and northeastern Pará to amplify or renew their breeding stocks, in order to avoid the significant loss of genetic diversity in the tambaqui farms of these regions.

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New pieces on genetic diversity and evolutionary history of hepatitis B virus: Characterization of the novel subgenotype F6

Infection Genetics and Evolution ◽

10.1016/j.meegid.2016.11.023 ◽

2017 ◽

Vol 47 ◽

pp. 140-142 ◽

Cited By ~ 2

Author(s):

Laura Noelia Mojsiejczuk ◽

Carolina Torres ◽

María Belén Pisano ◽

Viviana Re ◽

Rodolfo Héctor Campos ◽

...

Keyword(s):

Genetic Diversity ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Evolutionary History ◽

The Novel ◽

B Virus ◽

History Of

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Molecular Characterization of Verbascum anisophyllum (Scrophulariaceae) Genetic Resources Through Inter-Simple Sequence Repeat (ISSR) Markers

Natural Product Communications ◽

10.1177/1934578x1701200212 ◽

2017 ◽

Vol 12 (2) ◽

pp. 1934578X1701200 ◽

Cited By ~ 1

Author(s):

Galya Petrova ◽

Stefan Petrov ◽

Svetlana Bancheva

Keyword(s):

Genetic Diversity ◽

Genetic Resources ◽

Simple Sequence Repeat ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Sequence Repeat ◽

Data Book ◽

Simple Sequence

Verbascum (Mullein) flowers are highly valued as natural remedy for various respiratory diseases. Verbascum anisophyllum Murb. is a Balkan endemic, protected by law and included in the Bulgarian Red Data Book as “Critically Endangered”. Thus, a strict conservation policy and a reliable evaluation of its genetic resources are required, considering its narrow distribution range and the increasing risk from destruction of its habitats. Here, we used Inter-simple sequence repeat (ISSR) markers to characterize the genetic diversity and to assess the genetic differentiation between the existing populations of Verbascum anisophyllum in Bulgaria. The level of genetic diversity found herein clearly indicates a long-term potential for adaptability of this endangered plant. Our findings provide important knowledge of population genetic structure of this species, thus representing a strategy for its efficient conservation and utilization.

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The potential of landscape genomics approach in the characterization of adaptive genetic diversity in indigenous goat genetic resources: A South African perspective

Small Ruminant Research ◽

10.1016/j.smallrumres.2017.03.015 ◽

2017 ◽

Vol 150 ◽

pp. 87-92 ◽

Cited By ~ 4

Author(s):

Khanyisile Mdladla ◽

Edgar F. Dzomba ◽

Farai C. Muchadeyi

Keyword(s):

Genetic Diversity ◽

Genetic Resources ◽

South African ◽

Landscape Genomics ◽

African Perspective ◽

Indigenous Goat ◽

Adaptive Genetic Diversity

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Inventory and Characterization of Local Taro Genetic Resources in North Toraja

Buletin Plasma Nutfah ◽

10.21082/blpn.v24n2.2018.p115-124 ◽

2018 ◽

Vol 24 (2) ◽

pp. 115

Author(s):

Erina Septianti ◽

Sahardi Sahardi

Keyword(s):

Genetic Diversity ◽

Genetic Resources ◽

Growth Performance ◽

Sampling Method ◽

Morphological Characters ◽

Optimum Growth ◽

High Genetic Diversity ◽

Purposive Sampling

<p>Taro (Colocasia esculenta (L.) Schott) has been cultivated and used as an alternative food in Toraja. Local taro of North Toraja has high genetic diversity, nevertheless, they have not been well characterized. The objective of this study was to inventorying and characterizing the morphological characters of local taro from North Toraja, that could be a key component for registered and maintained as a wealth of local genetic resources. The study was conducted in North Toraja District which includes highland areas in Parodo Baruppu Village, Baruppu District and lowland areas in Mantirotiku Village, Rantepao District. Each region was selected based on initial surveys which showed that these areas were taro cultivation areas. The study was conducted from April to June 2016. Samples were selected based on purposive sampling method and were descriptively observed in-situ in the field on their optimum growth performance. Five local taro varieties were successfully inventoried from survey activities, namely Upe Ungu, Upe Kuning, Upe Putih, Monggo, and Bite. Those taros have similar characters on the shape and leave color but varied on the size and flesh fibre color of the corm. Local taro from North Toraja is widely used as snacks or vegetables as rice substitution. The advantage of North Toraja local taro is easy to cultivate and also has a savory taste, soft, good fragrant aroma, the corm skin after being boiled is very easy to peel, and the texture of the corm is smooth. The price of this local taro is quite expensive because of still difficult to find and have not been widely cultivated.</p>

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Molecular characterization of oil palmElaeis guineensisJacq. materials from Cameroon

Plant Genetic Resources ◽

10.1017/s1479262112000482 ◽

2013 ◽

Vol 11 (2) ◽

pp. 140-148 ◽

Cited By ~ 5

Author(s):

Diana Arias ◽

Carmenza Montoya ◽

Hernán Romero

Keyword(s):

Genetic Diversity ◽

Genetic Resources ◽

Molecular Characterization ◽

Oil Palm ◽

Elaeis Guineensis ◽

Low Frequency ◽

Molecular Data ◽

Ex Situ ◽

Geographical Regions

The narrow genetic base of existing commercial oil palm cultivars has prompted oil palm breeders to give increased importance to augmenting these genetic resources because the sustainable development of the crop depends largely on the availability of genetic diversity and its use. Therefore, the purpose of this study was to conduct a molecular characterization of anex situcollection of oil palmElaeis guineensisJacq. populations from the Republic of Cameroon using microsatellite molecular markers. Overall, 31 simple sequence repeats were polymorphic, with a total of 223 alleles, 78.4% of which were found at low frequency. The total genetic diversity was relatively high (HT= 0.673). The genetic differentiation between geographical regions was low (GST= 0.023,P= 0.001), and between families it was high (GST= 0.166,P= 0.001), showing greater variation between families than among geographical regions. The molecular data indicate that genetic diversity among the genotypes evaluated is mainly distributed within regions, suggesting that there is no isolation by geographical distance and that all the sampled individuals form a single diverse population. Therefore, it was concluded that a relatively low number of accessions (120 in the analysed case) that includes at least one representative of each family would allow us to efficiently collect almost the entire genetic diversity of Cameroon within the collection studied. This will allow for the efficient use of genetic resources and a reduction in morpho-agronomic characterization costs.

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Molecular characterization of Bulgarian livestock genetic resources: 1. Genetic diversity in Bulgarian grey cattle as revealed by microsatellite markers

Biotechnology in Animal Husbandry ◽

10.2298/bah0502035t ◽

2005 ◽

Vol 21 (5-6-2) ◽

pp. 35-42 ◽

Cited By ~ 8

Author(s):

Atanaska Teneva ◽

Elena Todorovska ◽

Nikolai Tyufekchiev ◽

Lasar Kozelov ◽

Atanas Atanassov ◽

...

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Genetic Resources ◽

Molecular Characterization

nema

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PRELIMINARY RESULTS CONCERNING THE PRESERVATION OF GENETICAL DIVERSITY OF DIFFERENT VEGETABLE VARIETIES AT USAMV CLUJ-NAPOCA

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:1357 ◽

1970 ◽

Vol 63 ◽

Author(s):

A. Maxim ◽

Rodica Sima ◽

A. Fiţiu ◽

M. Şandor ◽

Ruxandra Papp ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Resources ◽

Plant Genetic Resources ◽

Lettuce Seed ◽

Local Populations ◽

Lettuce Seeds ◽

Environment And Development ◽

Genetical Diversity ◽

International Plant

The biodiversity conservation was included in environment problems at the ONU conference for environment and development, from Rio de Janeiro (1992). Important parts of the conservation are traditional varieties and local populations of crop plants. The UE regulation 98/95 comes to protect the vegetal genetic resources. Unlike the other countries with advanced agriculture, Romania still has a rich genetic diversity of culture plants, especially in the zones that were non-cooperativized during the communism. However, if there will not be any proper measures of their conservation, these populations and varieties are in danger of extinction. Our objectives presume finding the small seed producers, collecting carrot, parsley and lettuce seeds, building experimental zones for the characterization of varieties and local populations as well as the conservation of their seeds at the Gene Bank Suceava and gene microbank from USAMV Cluj-Napoca, which is in the stage of project. In 2007 spring, 188 traditional varieties and local populations of carrot, parsley and lettuce seeds, from 69 producers within 15 counties of our country, especially from Transylvania were collected. The seeds were mainly from the agro alimentary markets collected. A questionnaire for every seed producer was filled in order to obtain information regarding: the tradition in the seed production, technology of culture, seeds sale, sex and age of the producers etc. Every variety has a unique code, after the proposal of FAO/IPGRI (International Plant Genetic Resources Institute), valid for all vegetal varieties. The obtained data confirm that vegetable genetic diversity, in the studied area, is big but the risk of extinction is high. The most important cause are the advanced age of traditional seed producers (about 70% of them are over 60 years old) and the recession of vegetable cultivated zones. In 2007 year, an agrobiologic characterization on carrot, parsley and lettuce was done and lettuce seed was obtained. The experiments will continue in 2008.

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