Optimization of high-performance capillary electrophoresis of plant growth regulators using the overlapping resolution mapping scheme

1991 ◽  
Vol 63 (20) ◽  
pp. 2222-2225 ◽  
Author(s):  
Sock Khim. Yeo ◽  
Chye Peng. Ong ◽  
Sam Fong Yau. Li
Keyword(s):  
Capillary Electrophoresis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
High Performance ◽  
Resolution Mapping ◽  
High Performance Capillary Electrophoresis ◽  
Mapping Scheme ◽  
Overlapping Resolution Mapping Scheme

scholarly journals Application of Plant Growth Regulators Modulates the Profile of Chlorogenic Acids in Cultured Bidens pilosa Cells

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 437
Author(s):  
Anza-Tshilidzi Ramabulana ◽  
Paul A. Steenkamp ◽  
Ntakadzeni E. Madala ◽  
Ian A. Dubery
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
High Performance ◽  
Total Phenolic ◽  
Chlorogenic Acids ◽  
In Vitro Production ◽  
Bidens Pilosa ◽  
Indirect Organogenesis

Plant cell culture offers an alternative to whole plants for the production of biologically important specialised metabolites. In cultured plant cells, manipulation by auxin and cytokinin plant growth regulators (PGRs) may lead to in vitro organogenesis and metabolome changes. In this study, six different combination ratios of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (BAP) were investigated with the aim to induce indirect organogenesis from Bidens pilosa callus and to investigate the associated induced changes in the metabolomes of these calli. Phenotypic appearance of the calli and total phenolic contents of hydromethanolic extracts indicated underlying biochemical differences that were investigated using untargeted metabolomics, based on ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC–qTOF–MS), combined with multivariate data analysis. The concentration and combination ratios of PGRs were shown to induce differential metabolic responses and, thus, distinct metabolomic profiles, dominated by chlorogenic acids consisting of caffeoyl- and feruloyl-derivatives of quinic acid. Although organogenesis was not achieved, the results demonstrate that exogenous application PGRs can be used to manipulate the metabolome of B. pilosa for in vitro production of specialised metabolites with purported pharmacological properties.

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