scholarly journals Application of Plant Growth Regulators Modulates the Profile of Chlorogenic Acids in Cultured Bidens pilosa Cells

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 437
Author(s):  
Anza-Tshilidzi Ramabulana ◽  
Paul A. Steenkamp ◽  
Ntakadzeni E. Madala ◽  
Ian A. Dubery
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
High Performance ◽  
Total Phenolic ◽  
Chlorogenic Acids ◽  
In Vitro Production ◽  
Bidens Pilosa ◽  
Indirect Organogenesis

Plant cell culture offers an alternative to whole plants for the production of biologically important specialised metabolites. In cultured plant cells, manipulation by auxin and cytokinin plant growth regulators (PGRs) may lead to in vitro organogenesis and metabolome changes. In this study, six different combination ratios of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (BAP) were investigated with the aim to induce indirect organogenesis from Bidens pilosa callus and to investigate the associated induced changes in the metabolomes of these calli. Phenotypic appearance of the calli and total phenolic contents of hydromethanolic extracts indicated underlying biochemical differences that were investigated using untargeted metabolomics, based on ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC–qTOF–MS), combined with multivariate data analysis. The concentration and combination ratios of PGRs were shown to induce differential metabolic responses and, thus, distinct metabolomic profiles, dominated by chlorogenic acids consisting of caffeoyl- and feruloyl-derivatives of quinic acid. Although organogenesis was not achieved, the results demonstrate that exogenous application PGRs can be used to manipulate the metabolome of B. pilosa for in vitro production of specialised metabolites with purported pharmacological properties.

scholarly journals Protocols for Adventitious Regeneration of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica ‘Jugana’

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1155
Author(s):  
Júlia Hunková ◽  
Monika Szabóová ◽  
Alena Gajdošová
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Adventitious Shoots ◽  
Culture Conditions ◽  
Indirect Organogenesis ◽  
Adventitious Regeneration ◽  
Amelanchier Alnifolia ◽  
Indole 3 Acetic Acid

The aim of this work was to assess the regeneration capacity of Amelanchier alnifolia var. cusickii and Lonicera kamtschatica cv. ‘Jugana’ from different types of explants under various hormonal treatments. The whole leaves, petioles, and internodal segments of in vitro plants were examined as explants. Several plant growth regulators (cytokinins and auxins) were evaluated for their ability to induce adventitious regeneration. Direct and indirect organogenesis was achieved under certain culture conditions in both species. The frequency of shoot regeneration was strongly dependent on concentrations of plant growth regulators in the induction media (L.kamtschatica ‘Jugana’) or concentrations of plant growth regulators in the induction media and type of explant (A. alnifolia var. cusickii). Results showed that leaves were not suitable explants for A. alnifolia var. cusickii. Both species were able to regenerate shoots from internodal segments and petioles. The highest induction of shoots was obtained on Murashige and Skoog (MS) medium enriched with 2 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-butyric acid (IBA) for Amelanchier alnifolia and with 1 mg/L TDZ and 0.2 mg/L indole-3-acetic acid (IAA) for L. kamtschatica ‘Jugana’. Obtained adventitious shoots were further proliferated in order to investigate their multiplication capacity. The multiplication of shoots was successful in all cultivars, with the best results reported in A. alnifolia var. cusickii (7.07 shoots/explant on average).

scholarly journals Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 124
Author(s):  
Anna A. Erst ◽  
Anastasia A. Petruk ◽  
Andrey S. Erst ◽  
Denis A. Krivenko ◽  
Nadezhda V. Filinova ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Plant Growth ◽  
Design Of Experiments ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Biomass Accumulation ◽  
Callus Cultures ◽  
Rhodiola Rosea ◽  
Total Phenolic

Rhodiola rosea L. is a valuable medicinal plant with adaptogenic, neuroprotective, antitumor, cardioprotective, and antidepressant effects. In this study, design of experiments methodology was employed to analyze and optimize the interacting effects of mineral compounds (concentration of NO3− and the ratio of NH4+ to K+) and two plant growth regulators [total 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentration and the ratio of BAP to NAA] on the growth and the production of total phenolic compounds (TPCs) in R. rosea calluses. The overall effect of the model was highly significant (p < 0.0001), indicating that NH4+, K+, NO3−, BAP, and NAA significantly affected growth. The best callus growth (703%) and the highest production of TPCs (75.17 mg/g) were achieved at an NH4+/K+ ratio of 0.33 and BAP/NAA of 0.33, provided that the concentration of plant growth regulators was 30 μM and that of NO3− was ≤40 mM. According to high-performance liquid chromatography analyses of aerial parts (leaves and stems), in vitro seedlings and callus cultures of R. rosea contain no detectable rosarin, rosavin, rosin, and cinnamyl alcohol. This is the first report on the creation of an experiment for the significant improvement of biomass accumulation and TPC production in callus cultures of R. rosea.

scholarly journals Effect of plant growth regulators and explants on callus induction and study of antioxidant potentials and phenolic metabolites in Salvia tebesana Bunge

2020 ◽  
Vol 44 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Niloofar Hemmati ◽  
Monireh Cheniany ◽  
Ali Ganjeali
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Phenolic Acids ◽  
Growth Regulators ◽  
Callus Induction ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Total Phenolic ◽  
Scale Production

This study was undertaken to investigate the effect of different plant growth regulators (PGRs) on callus induction in Salvia tebesana explants grown in vitro and to evaluate the content of secondary phenolic compounds and their antioxidant potential. The explants (shoot apical meristem, leaf and petiole) were dissected from an 8-week-old plant of S. tebesana growing in vitro and cultured on MS media containing different concentrations of 2,4-D (0, 0.5, 1, 1.5 and 2 mg L-1), NAA (0, 0.5 and 1 mg L-1) and BAP (0, 0.5 and 1 mg L-1), either alone or in a blend with each other. Morphological characteristics of the callus (consistency and colour), biomass increase based on fresh and dry weight and the percentage of induction were recorded after 56 days. Levels of total phenols, ortho-diphenols, phenolic acids, flavonoids, proanthocyanidins and flavonols of callus, as well as antioxidant activities, were evaluated in vitro. The maximum callus formation (100%) was obtained from shoot apical meristem on MS medium supplemented with 0.5 and 1.5 mg L-1 2,4-D + 1 mg L-1 BAP and with 1 and 1.5 mg L-1 2,4-D + 0.5 mg L-1 BAP, whereas the highest fresh (15.06 ? 0.88 g) and dry (0.33 ? 0.02 g) weights of call were observed in a medium containing1.5 mg L-1 2,4-D + 0.5 mg L-1 NAA. It was noted that MS media augmented with combined PGRs had the highest accumulation of polyphenols, phenolic acids and flavonoid compounds, with levels of content varying in the following order: 2,4-D + BAP > NAA + BAP > 2,4-D + NAA. Strong linear correlations were established between total phenolic content of callus extracts and results of the DPPH and FRAP assays (r2 = 0.896 and r2 = 0.946, p < 0.01, respectively). The obtained results suggest that the described method could be utilised as a tool for large-scale production of medicinal metabolites of S. tebesana by tissue culture.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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