Micellar catalysis in kinetic multicomponent analysis: simultaneous determination of binary mixtures of cyanide, sulfide, and sulfite ions

Two spectrometric methods have been developed for quantitative simultaneous determination of procaine hydrochloride (PH·HCl), procainamide hydrochloride (PHA·HCl) and lidocaine (Lid) from synthetic mixture. The methods employed are first derivative spectrometry, using zero crossing method and multicomponent analysis which is based on the additivity law. Using first derivative spectrometry, the wavelength selected for the quantitative determination of PH·HCl was 237 nm for Lid was 242 nm and for PHA·HCl was 290 nm in mixture. The method is linear when the concentration ranged between 6.62-9.93 μg/mL for PH·HCl, 6.43-9.64 for PHA·HCl and 5.56-8.35 for Lid. The multicomponent analysis is a direct method and involves the absorbance measurements of at three different wavelengths. The molar absorption coefficients values were calculated at each wavelength and the concentration of PH·HCl, PHA·HCl and Lid from mixture was determined by solving matrix using Cramer's rule. The recovery of each compound in mixture was calculated and it is 101.4 % for PH·HCl, 100.4 % for PHA·HCl and 98.4 % for Lid.

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SELECTIVE REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FOR THE SIMULTANEOUS DETERMINATION OF SOME PHARMACEUTICAL BINARY MIXTURES CONTAINING NSAIDS

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826076.2011.629390 ◽

2012 ◽

Vol 35 (15) ◽

pp. 2188-2202 ◽

Cited By ~ 13

Author(s):

Michael E. El-Kommos ◽

Niveen A. Mohamed ◽

Ahmed F. Abdel Hakiem

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Simultaneous Determination ◽

Binary Mixtures ◽

High Performance ◽

Reversed Phase

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Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer.

Clinical Chemistry ◽

10.1093/clinchem/30.3.373 ◽

1984 ◽

Vol 30 (3) ◽

pp. 373-379 ◽

Cited By ~ 52

Author(s):

A Zwart ◽

A Buursma ◽

E J van Kampen ◽

W G Zijlstra

Keyword(s):

Simultaneous Determination ◽

Separate Determination ◽

Overdetermined System ◽

Chemical Research ◽

Chemical Laboratory ◽

Multicomponent Analysis ◽

Hemoglobin Derivatives ◽

Clinical Chemical ◽

Human Blood Samples

Abstract We describe a method for the simultaneous determination of the five clinically relevant hemoglobin derivatives (Hb, HbO2, HbCO, Hi, SHb) in a blood sample by means of a reversed-optics spectrophotometer (Hewlett-Packard HP8450 A UV/Vis). A built-in computer program is used for multicomponent analysis in an overdetermined system, i.e., a system in which the number of independent equations used exceeds the number of unknowns to be determined. First, the spectra of the five hemoglobin derivatives are measured in a series of different human blood samples. Thereafter, the multicomponent method for the simultaneous determination of the five hemoglobin derivatives is tested by comparison with conventional methods for the separate determination of oxygen saturation, HbCO, Hi, and SHb fractions. The multicomponent (multiwavelength) method is sufficiently reliable, accurate, and easy to justify its use in physiological chemical research as well as its routine application in the clinical chemical laboratory.

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