Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer.

1984 ◽  
Vol 30 (3) ◽  
pp. 373-379 ◽  
Author(s):  
A Zwart ◽  
A Buursma ◽  
E J van Kampen ◽  
W G Zijlstra
Keyword(s):  
Simultaneous Determination ◽  
Separate Determination ◽  
Overdetermined System ◽  
Chemical Research ◽  
Chemical Laboratory ◽  
Multicomponent Analysis ◽  
Hemoglobin Derivatives ◽  
Clinical Chemical ◽  
Human Blood Samples

Abstract We describe a method for the simultaneous determination of the five clinically relevant hemoglobin derivatives (Hb, HbO2, HbCO, Hi, SHb) in a blood sample by means of a reversed-optics spectrophotometer (Hewlett-Packard HP8450 A UV/Vis). A built-in computer program is used for multicomponent analysis in an overdetermined system, i.e., a system in which the number of independent equations used exceeds the number of unknowns to be determined. First, the spectra of the five hemoglobin derivatives are measured in a series of different human blood samples. Thereafter, the multicomponent method for the simultaneous determination of the five hemoglobin derivatives is tested by comparison with conventional methods for the separate determination of oxygen saturation, HbCO, Hi, and SHb fractions. The multicomponent (multiwavelength) method is sufficiently reliable, accurate, and easy to justify its use in physiological chemical research as well as its routine application in the clinical chemical laboratory.

scholarly journals Simultaneous determination of procaine hydrochloride, procainamide hydrochloride and lidocaine by molecular absorption spectrometry

2019 ◽  
pp. 318-324
Author(s):  
Nicoleta Mirela Marin ◽  
Gheorghe Batrinescu ◽  
Mihai Nita-Lazar ◽  
Luoana Florentina Pascu ◽  
Carol Blaziu Lehr
Keyword(s):  
Simultaneous Determination ◽  
Direct Method ◽  
Absorption Spectrometry ◽  
Procaine Hydrochloride ◽  
Zero Crossing ◽  
Multicomponent Analysis ◽  
First Derivative ◽  
Cramer’S Rule ◽  
Derivative Spectrometry

Two spectrometric methods have been developed for quantitative simultaneous determination of procaine hydrochloride (PH·HCl), procainamide hydrochloride (PHA·HCl) and lidocaine (Lid) from synthetic mixture. The methods employed are first derivative spectrometry, using zero crossing method and multicomponent analysis which is based on the additivity law. Using first derivative spectrometry, the wavelength selected for the quantitative determination of PH·HCl was 237 nm for Lid was 242 nm and for PHA·HCl was 290 nm in mixture. The method is linear when the concentration ranged between 6.62-9.93 μg/mL for PH·HCl, 6.43-9.64 for PHA·HCl and 5.56-8.35 for Lid. The multicomponent analysis is a direct method and involves the absorbance measurements of at three different wavelengths. The molar absorption coefficients values were calculated at each wavelength and the concentration of PH·HCl, PHA·HCl and Lid from mixture was determined by solving matrix using Cramer's rule. The recovery of each compound in mixture was calculated and it is 101.4 % for PH·HCl, 100.4 % for PHA·HCl and 98.4 % for Lid.

Micellar catalysis in kinetic multicomponent analysis: simultaneous determination of binary mixtures of cyanide, sulfide, and sulfite ions

1993 ◽  
Vol 65 (14) ◽  
pp. 1897-1902 ◽  
Author(s):  
Victoria. Gonzalez ◽  
Bernardo. Moreno ◽  
Dolores. Sicilia ◽  
Soledad. Rubio ◽  
Dolores. Perez-Bendito
Keyword(s):  
Simultaneous Determination ◽  
Binary Mixtures ◽  
Micellar Catalysis ◽  
Multicomponent Analysis ◽  
Sulfite Ions

Simultaneous Determination of 5- and 6-APB in Blood, Other Body Fluids, Hair and Various Tissues by HPLC-MS/MS

2021 ◽  
Author(s):  
Vanessa Hofmann ◽  
Tom Richard Sundermann ◽  
Aysche Landmann ◽  
Stefanie Rechtsteiner ◽  
Georg Schmitt ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Chromatographic Method ◽  
Simultaneous Detection ◽  
Body Fluids ◽  
New Psychoactive Substances ◽  
Fatal Intoxication ◽  
Psychoactive Substances ◽  
Human Blood Samples

Abstract 5-(2-Aminopropyl)benzofuran (5-APB) and 6-(2-aminopropyl)benzofuran (6-APB) are benzofuran analogues of amphetamine and belong to the category of new psychoactive substances. Despite already published fatal 5- and 6-APB intoxication after consumption of both substances in most cases, no sensitive method for the simultaneous detection and quantification of these new psychoactive compounds in human blood samples has yet been developed. Therefore, an easy and fast sample preparation and specific high-performance liquid chromatography and tandem mass spectrometry methods for the determination of both substances in blood were established and validated. In a fatal intoxication in 2017 at the Institute of Forensic and Traffic Medicine in Heidelberg, Germany, concentrations of 850 (5-APB) and 300 ng/mL (6-APB) were determined in peripheral blood. Besides, other body fluids (central blood, urine and bile), hair and various tissues were examined to verify the presence of both compounds and to gain first insights into their distribution. In this publication, we show a method for the simultaneous determination of 5- and 6-APB in human samples by a chromatographic method and to investigate their distribution in the human body.

scholarly journals Simultaneous determination of hemoglobin derivatives, oxygen content, oxygen capacity, and oxygen saturation in 10 microliters of whole blood.

1977 ◽  
Vol 23 (7) ◽  
pp. 1215-1225 ◽  
Author(s):  
L Rossi-Bernardi ◽  
M Perella ◽  
M Luzzana ◽  
M Samaja ◽  
I Raffaele
Keyword(s):  
Oxygen Saturation ◽  
Simultaneous Determination ◽  
Oxygen Content ◽  
Oxygen Pressure ◽  
Whole Blood ◽  
Sample Volume ◽  
New Method ◽  
Hemoglobin Derivatives ◽  
Oxygen Capacity

Abstract We describe a new method for simultaneous determination of four hemoglobin derivatives (deoxyhemoglobin, Hb; oxyhemoglobin, HbO2; methemoglobin, Hb+; and carbon monoxide hemoglobin, HbCO) and total oxygen content in 10 microliters of whole blood. Percentage HbO2, HbCO, Hb+, total hemoglobin (Hbt), and oxygen capacity can also be obtained from the experimental data by simple calculations. Total analysis time is 1 min. Blood is diluted 100-fold with a buffer contained in a quasi-anaerobic cuvette, where simultaneous measurements of oxygen pressure (by a po2 electrode) and adsorbance (at 497, 565, and 620 nm) are made. The decrease of oxygen pressure, as recorded by the oxygen electrode, is proportional to the amount of deoxyhemoglobin. The concentrations of HbO2, HbCO, and Hb+ can be obtained from absorbance measurements at the specified wavelengths. The new method eliminates the use of short-path optical cells and, due to the low sample volume requirement, makes possible the automated measurement of hemoglobin derivatives and oxygen saturation in arterialized capillary blood.

A general computer program for the extended plate overlap algorithm

1978 ◽  
Vol 48 ◽  
pp. 287-293 ◽  
Author(s):  
Chr. de Vegt ◽  
E. Ebner ◽  
K. von der Heide
Keyword(s):  
Computer Program ◽  
Simultaneous Determination ◽  
General Computer Program ◽  
General Computer

In contrast to the adjustment of single plates a block adjustment is a simultaneous determination of all unknowns associated with many overlapping plates (star positions and plate constants etc. ) by one large adjustment. This plate overlap technique was introduced by Eichhorn and reviewed by Googe et. al. The author now has developed a set of computer programmes which allows the adjustment of any set of contemporaneous overlapping plates. There is in principle no limit for the number of plates, the number of stars, the number of individual plate constants for each plate, and for the overlapping factor.

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