scholarly journals Rapid Screening and Identification of Glycosides in Biological Samples Using Energy-Gradient Neutral Loss Scan and Liquid Chromatography Tandem Mass Spectrometry

2004 ◽  
Vol 76 (11) ◽  
pp. 3249-3249
Author(s):  
Jun Qu ◽  
Qionglin Liang ◽  
Guoan Luo ◽  
Yiming Wang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Neutral Loss ◽  
Rapid Screening ◽  
Tandem Mass ◽  
Neutral Loss Scan ◽  
Chromatography Tandem Mass Spectrometry ◽  
Energy Gradient ◽  
Liquid Chromatography Tandem Mass ◽  
Screening And Identification

scholarly journals LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

Marine Drugs ◽  
2020 ◽  
Vol 18 (10) ◽  
pp. 497
Author(s):  
Marvin Wellkamp ◽  
Francisco García-Camacho ◽  
Lorena M. Durán-Riveroll ◽  
Jan Tebben ◽  
Urban Tillmann ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Method Development ◽  
Neutral Loss ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Fish Kills ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Fragmentation Patterns

Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.

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