Fundamental Equation of Dual-Mode Gradient Elution in Liquid Chromatography Involving Simultaneous Changes in Flow Rate and Mobile-Phase Composition

2007 ◽  
Vol 79 (10) ◽  
pp. 3888-3893 ◽  
Author(s):  
A. Pappa-Louisi ◽  
P. Nikitas ◽  
P. Balkatzopoulou ◽  
G. Louizis
Keyword(s):  
Liquid Chromatography ◽  
Phase Composition ◽  
Flow Rate ◽  
Mobile Phase ◽  
Fundamental Equation ◽  
Gradient Elution ◽  
Mobile Phase Composition ◽  
Dual Mode

Development and validation of high-performance liquid chromatography method for the simultaneous monitoring of pantoprazole sodium sesquihydrate and domperidone maleate in plasma and its application to pharmacokinetic study

2020 ◽  
Vol 32 (3) ◽  
pp. 157-165
Author(s):  
Ghulam Abbas ◽  
Malik Saadullah ◽  
Akhtar Rasul ◽  
Shahid Shah ◽  
Sajid Mehmood Khan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phase Composition ◽  
Flow Rate ◽  
Retention Time ◽  
Mobile Phase ◽  
High Performance ◽  
Mobile Phase Composition ◽  
Chromatography Method ◽  
Surface Design

A sensitive, inexpensive high-performance liquid chromatography–ultraviolet detection (HPLC–UV) method has been developed and validated for the simultaneous monitoring of pantoprazole sodium sesquihydrate (PSS) and domperidone maleate (DM) in rabbit plasma on a C18 column with UV detection at 285 nm. Box–Behnken design was used with 3 independent variables, namely, flow rate (X1), mobile phase composition (X2), and phosphate buffer pH (X3), which were used to design mathematical models. Response surface design was applied to optimize the dependent variables, i.e., retention time (Y1 and Y2) and percentage recoveries (Y3 and Y4) of PSS and DM. The method was sensitive and reproducible over 1.562 to 25 μg/mL. The effect of the quadratic outcome of flow rate, mobile phase composition, and buffer pH on retention time (p ˂ 0.001) and percentage recoveries of PSS and DM (p = 0.0016) were significant. The regression values obtained from analytical curve of PSS and DM were 0.999 and 0.9994, respectively. The percentage recoveries of PSS and DM were ranged from 94.5 to 100.41% and 94.77 to 100.31%, respectively. The developed method was applied for studying the pharmacokinetics of PSS and DM. The Cmax of test and reference formulations were 48.06 ± 0.347 μg/mL and 46.31 ± 0.398 μg/mL for PSS, and 15.11 ± 1.608 μg/mL and 12.06 ± 1.234 μg/mL for DM, respectively.

Theory and Application of the Two-Mode Gradient Elution in Liquid Chromatography Involving Simultaneous Changes in Temperature and Mobile-Phase Composition

2008 ◽  
Vol 80 (14) ◽  
pp. 5508-5514 ◽  
Author(s):  
P. Nikitas ◽  
A. Pappa-Louisi ◽  
K. Papachristos ◽  
C. Zisi
Keyword(s):  
Liquid Chromatography ◽  
Phase Composition ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Mobile Phase Composition

scholarly journals Description of the Retention and Peak Profile for Chromolith Columns in Isocratic and Gradient Elution Using Mobile Phase Composition and Flow Rate as Factors

2014 ◽  
Vol 1 (4) ◽  
pp. 194-210 ◽  
Author(s):  
Elsa Cabo-Calvet ◽  
Casandra Ortiz-Bolsico ◽  
Juan Baeza-Baeza ◽  
María García-Alvarez-Coque
Keyword(s):  
Phase Composition ◽  
Flow Rate ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Mobile Phase Composition ◽  
Isocratic And Gradient Elution
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