In Vivo Termini Amino Acid Labeling for Quantitative Proteomics

2011 ◽  
Vol 83 (15) ◽  
pp. 6026-6033 ◽  
Author(s):  
Ai-Ying Nie ◽  
Lei Zhang ◽  
Guo-Quan Yan ◽  
Jun Yao ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Quantitative Proteomics ◽  
Amino Acid Labeling

scholarly journals BIOSYNTHESIS IN ISOLATED ACETABULARIA CHLOROPLASTS

1972 ◽  
Vol 54 (2) ◽  
pp. 279-294 ◽  
Author(s):  
David C. Shephard ◽  
Wendy B. Levin
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Technical Problems ◽  
Hydrolyzed Protein ◽  
Protein Amino Acids ◽  
Amino Acid Labeling ◽  
Labeling Pattern

The ability of chloroplasts isolated from Acetabulana mediterranea to synthesize the protein amino acids has been investigated. When this chloroplast isolate was presented with 14CO2 for periods of 6–8 hr, tracer was found in essentially all amino acid species of their hydrolyzed protein Phenylalanine labeling was not detected, probably due to technical problems, and hydroxyproline labeling was not tested for The incorporation of 14CO2 into the amino acids is driven by light and, as indicated by the amount of radioactivity lost during ninhydrin decarboxylation on the chromatograms, the amino acids appear to be uniformly labeled. The amino acid labeling pattern of the isolate is similar to that found in plastids labeled with 14CO2 in vivo. The chloroplast isolate did not utilize detectable amounts of externally supplied amino acids in light or, with added adenosine triphosphate (ATP), in darkness. It is concluded that these chloroplasts are a tight cytoplasmic compartment that is independent in supplying the amino acids used for its own protein synthesis. These results are discussed in terms of the role of contaminants in the observed synthesis, the "normalcy" of Acetabularia chloroplasts, the synthetic pathways for amino acids in plastids, and the implications of these observations for cell compartmentation and chloroplast autonomy.

scholarly journals Quantitative proteomics by amino acid labeling identifies novel NHR-49 regulated proteins inC. elegans

Worm ◽  
2012 ◽  
Vol 1 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Julius Fredens ◽  
Nils J. Færgeman
Keyword(s):  
Amino Acid ◽  
Quantitative Proteomics ◽  
Amino Acid Labeling

Quantitative Proteomics by Amino Acid Labeling in Foot-and-Mouth Disease Virus (FMDV)-Infected Cells

2012 ◽  
Vol 12 (1) ◽  
pp. 363-377 ◽  
Author(s):  
Yu Ye ◽  
Guangrong Yan ◽  
Yongwen Luo ◽  
Tiezhu Tong ◽  
Xiangtao Liu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Disease Virus ◽  
Quantitative Proteomics ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Infected Cells ◽  
Amino Acid Labeling

Quantitative proteomics by amino acid labeling in C. elegans

2011 ◽  
Vol 8 (10) ◽  
pp. 845-847 ◽  
Author(s):  
Julius Fredens ◽  
Kasper Engholm-Keller ◽  
Anders Giessing ◽  
Dennis Pultz ◽  
Martin Røssel Larsen ◽  
...  
Keyword(s):  
Amino Acid ◽  
Quantitative Proteomics ◽  
C Elegans ◽  
Amino Acid Labeling

scholarly journals Noncanonical Amino Acid Labeling in Vivo to Visualize and Affinity Purify Newly Synthesized Proteins in Larval Zebrafish

2011 ◽  
Vol 3 (1) ◽  
pp. 40-49 ◽  
Author(s):  
Flora I. Hinz ◽  
Daniela C. Dieterich ◽  
David A. Tirrell ◽  
Erin M. Schuman
Keyword(s):  
Amino Acid ◽  
Larval Zebrafish ◽  
Amino Acid Labeling ◽  
Noncanonical Amino Acid

Global in vivo terminal amino acid labeling for exploring differential expressed proteins induced by dialyzed serum cultivation

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4497-4504 ◽  
Author(s):  
Li-Qi Xie ◽  
Ai-Ying Nie ◽  
Shu-Jun Yang ◽  
Chao Zhao ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Throughput ◽  
Trypsin Digestion ◽  
Metabolic Labeling ◽  
Terminal Amino Acid ◽  
Terminal Amino ◽  
Amino Acid Labeling ◽  
Dialyzed Serum

An accurate and high throughput isobaric MS2 quantification strategy based on metabolic labeling and trypsin digestion.

scholarly journals Quantitative Proteomics: Measuring Protein Synthesis Using15N Amino Acid Labeling in Pancreatic Cancer Cells

2009 ◽  
Vol 81 (2) ◽  
pp. 764-771 ◽  
Author(s):  
Yingchun Zhao ◽  
Wai-Nang Paul Lee ◽  
Shu Lim ◽  
Vay Liang Go ◽  
Jing Xiao ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Cancer Cells ◽  
Quantitative Proteomics ◽  
Pancreatic Cancer Cells ◽  
Amino Acid Labeling

Site Specific Incorporation of Amino Acid Analogues into Proteins In Vivo

2000 ◽  
Author(s):  
Anne K. Kowal ◽  
Caroline Kohrer ◽  
Uttam L. RajBhandary
Keyword(s):  
Amino Acid ◽  
Site Specific ◽  
Amino Acid Analogues
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