Dual X-Ray Image under Neural Network Adopted in Comparison of Efficacy and Safety of Deep Hydrolyzed Protein Milk Powder and Parenteral Nutrition in Intervention of Neonatal Noninfectious Abdominal Distension

Objective. The aim of this study was to explore the application value of double X-ray image based on neural network in comparison of the efficacy and safety of deep-water proteolytic milk powder and parenteral nutrition in the intervention of neonatal noninfectious abdominal distention. Methods. Clinical data of 58 neonates diagnosed with noninfectious abdominal distention were retrospectively analyzed. 2D-3D registration was simplified into two steps by decomposing spatial rigid-body transformation parameters into two planes, including 2D-2D approximate rigid-body registration and single-parameter 2D-3D rigid-body registration. Then, the convolution neural network was used to fit the nonlinear mapping relationship between the residual of X-ray images and the corresponding attitude differences of children, and the residual regression spatial rigid-body transformation parameters of the X-DRR image pairs were obtained. Noninfectious abdominal distention was diagnosed in all neonates, of which 28 neonates were treated with deep hydrolyzed protein milk powder. Another 30 neonates who received parenteral nutrition support were set as control group. All newborns received two weeks of treatment. The total effective rate, birth weight recovery, weight growth rate, intestinal feeding recovery time, and incidence of feeding intolerance were compared between the two groups. Results. Spatial coordinate decomposition using double X-ray can simplify the mapping relationship between spatial coordinate transformation and X-DRR residual image. Compared with the gray level iterative optimization registration algorithm, the registration accuracy and speed were significantly improved. The total effective rate in the treatment group (92.86%) was significantly higher than that in the control group (9%). The recovery time of birth weight, intestinal feeding recovery time, and meconium excretion time were significantly shorter than those in the control group, and the body weight in the treatment group increased faster than that in the control group ( P < 0.05 ). In addition, the incidence of feeding intolerance was 3.57% (1/28) in the treatment group and 36.36% (8/22) in the control group, which was significantly lower than that in the treatment group ( P < 0.05 ). Conclusion. After data training, the network can complete accurate double ray registration in 0.04 s. Deep hydrolyzed protein milk powder had remarkable therapeutic effect on neonates, with no infective abdominal distention, fast recovery, and low incidence of feeding intolerance, which was safe and reliable in clinical application.

Nutritious mushroom protein crisp – healthy alternative to starchy snack

Global salty snacks category had reached USD 137 billion in sales in 2018. Due to growing health concerns and awareness, consumers are looking for healthy snack choices by avoiding ingredients such as fat, sugar, cholesterol, and sodium and selecting baked and salt free multigrain chips. A sizable number of consumers are concerned about snack nutrition and look for quality ingredients and minimally processed foods called as “Good Health Snack (GHS)”. In this work, we present the development of method of producing and testing mushrooms protein crisps (MPC), a healthy alternative to conventional starchy snacks that are rich in protein, nutraceutical compounds, minerals, vitamin, dietary fiber, and immunity inducing beta-glucans. The methods of producing MPC with different seasoning and hydrolyzed protein, calorie, nutritional and chemical composition, consumer response, shelf life after packing and market analysis are described. These systematic studies will help to market potential of this product which is a healthy alternative to other calorie rich snacks sold in the market benefiting the consumers. Graphical abstract

The Involvement of Macrophage Colony Stimulating Factor on Protein Hydrolysate Injection Mediated Hematopoietic Function Improvement

Protein hydrolysate injection (PH) is a sterile solution of hydrolyzed protein and sorbitol that contains 17 amino acids and has a molecular mass of 185.0–622.0 g/mol. This study investigated the effect of PH on hematopoietic function in K562 cells and mice with cyclophosphamide (CTX)-induced hematopoietic dysfunction. In these myelosuppressed mice, PH increased the number of hematopoietic cells in the bone marrow (BM) and regulated the concentration of several factors related to hematopoietic function. PH restored peripheral blood cell concentrations and increased the numbers of hematopoietic stem cells and progenitor cells (HSPCs), B lymphocytes, macrophages, and granulocytes in the BM of CTX-treated mice. Moreover, PH regulated the concentrations of macrophage colony stimulating factor (M-CSF), interleukin (IL)-2, and other hematopoiesis-related cytokines in the serum, spleen, femoral condyle, and sternum. In K562 cells, the PH-induced upregulation of hematopoiesis-related proteins was inhibited by transfection with M-CSF siRNA. Therefore, PH might benefit the BM hematopoietic system via the regulation of M-CSF expression, suggesting a potential role for PH in the treatment of hematopoietic dysfunction caused by cancer therapy.

Fish hydrolysate supplemented diet improved feed efficiency and growth of coral trout (Plectropomus leopardus)

Fish hydrolysate contains hydrolyzed protein which comprised of peptides and free amino acids that are easily digested and absorbed by fish. This study aimed to determine the effectiveness of fish hydrolysate in improving feed efficiency and growth of coral trout. Five experimental diets were formulated to contain 47% protein and 12% lipid with different sources and levels of fish hydrolysate. Diet-1 and diet-2 were added with crustacean hydrolysate at 2% and 4% levels, respectively. Whereas diet-3 and diet-4 were supplemented with 2% and 4% of tuna hydrolysate. Diet-5 was formulated without fish hydrolysate supplementation (control). The diets were prepared as dry pellet with 3 mm diameter. Coral trout juveniles were produced in the hatchery of Institute for Mariculture Research and Fisheries Extension (IMRAFE), Gondol-Bali with the average body weight of 7.6 ± 0.9 g. The juveniles were maintained in 15 fiber tanks, 400L in volume, with a density of 40 fish/tank. The experiment was designed with a completely randomized design (CRD) with 5 treatments (different diets) and 3 replications for each treatment. The fish were fed the experimantal diets three times every day at satiation level for 84 days of feeding experiment. Results of the experiment showed that increased supplementation of fish hydrolysate from 2% to 4% in diet tended to improve fish growth performance. However, the best growth performance, in term of weight gain (WG) and specific growth rate (SGR), was obtained in fish fed diet supplemented with 4% crustacean hydrolysate. This growth performance was significantly higher than of the other treatments (P<0.05). The best feed efficiency (FE) and protein efficiency ratio (PER) were also obtained in fish fed diet supplemented with 4% crustacean hydrolysate. The results of the present study showed that supplementation of 4% crustacean hydrolysate in diet effectively improved the growth and feed efficiency of coral trout.

Rheological Study of an Extruded Fish Diet with the Addition of Hydrolyzed Protein Flour

The extrusion of food for human and animal consumption is a unit operation that includes mixing, shearing, and force to the materials related to the rheological properties of the materials in the extruder. The present work aims to study the rheological behavior of an extruded fish diet incorporating hydrolyzed protein flour (HPF) processed by extrusion. The measurement was carried out online with a slit die rheometer, defining the rheological models and parameters that fit the process. During the extrusion process, the raw materials used were hydrolyzed protein flour, fish meal, and cassava starch. For the results, the evaluated treatments were adjusted to the Power Law, where an increase in the shear rate decreases the viscosity of the material, corresponding to a pseudoplastic behavior. The incorporation of hydrolyzed protein flour presented a significant effect on the value of n and Klp, increasing the viscosity with the increase in the percentage of inclusion of HPF. The models obtained for the prediction of the viscosity are adjusted to the system’s changes in shear rate, temperature, and humidity. The importance of the study lies in the fact that the diet developed can be applied to feed fish, and production is currently being scaled up to pilot plants for direct use by some producer communities.

Rheological Study of an Extruded Fish Diet with the Addition of Hydrolyzed Protein Meal

The extrusion of food for human and animal consumption is a unit operation that includes mixing, shearing and force to the materials related to the rheological properties of the materials in the extruder. The present work aims to study the rheological behavior of an extruded fish diet incorporating hydrolyzed protein flour processed by extrusion. The measurement was carried out online with a slit die rheometer, defining the rheological models and parameters that fit the process. During the extrusion process, the raw materials used were hydrolyzed protein meal, fish meal and cassava starch. For the results, the evaluated treatments were adjusted to the Power Law, where an increase in the shear rate decreases the viscosity of the material, corresponding to a pseudoplastic behavior. The incorporation of hydrolyzed protein flour presented a significant effect on the value of n and Klp, increasing the viscosity with the increase in the percentage of inclusion of HPH. The models obtained for the prediction of the viscosity are adjusted to the changes in shear rate, temperature and humidity of the system.

Infant Formulas: A Long Story

The ideal feeding for infants is the breast milk because it has a balanced nutritional composition, which includes all essential nutrients, in addition to many bioactive factors that contribute to the growth and development of the child, as well as to the maturation of the gastrointestinal tract. Among them are immunological factors, antimicrobials and anti-inflammatory components, digestive enzymes, various types of hormones, and growth factors. If human milk is not available, there is an indication of infant formulas that should follow the recommendations of the Codex Alimentarius of the Food and Agriculture Organization/World Health Organization (WHO). In a century of history, infant formulas have gone from a simple combination of cow milk (evaporated or condensed) and water to highly sophisticated products, elaborated by very refined technological processes to produce lactose-free, antiregurgitation, based on soy protein, hydrolyzed protein in various grades, and only amino acids formulas. The major milestones in the modification of infant formulas were the incorporation of nutrients/ingredients such as: iron, nucleotides, alpha lactalbumin, long-chain polyunsaturated fatty acids, prebiotics, probiotics, postbiotics, oligosaccharides similar to human milk, lactoferrin, and milk fat globule membrane. Many of these ingredients have shown benefits on the immunological system. Despite the technological advances, breast milk remains irreplaceable, being the gold standard for infant feeding.