Development of Atom Transfer Radical Polymer-Modified Gold Nanoparticle-Based Enzyme-Linked Immunosorbent Assay (ELISA)

2014 ◽  
Vol 86 (20) ◽  
pp. 10021-10024 ◽  
Author(s):  
Feng Chen ◽  
Shike Hou ◽  
Qingsheng Li ◽  
Haojun Fan ◽  
Rong Fan ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Atom Transfer ◽  
Atom Transfer Radical Polymer

An enzyme-linked immunosorbent assay and a gold-nanoparticle based immunochromatographic test for amatoxins using recombinant antibody

2016 ◽  
Vol 183 (7) ◽  
pp. 2211-2219 ◽  
Author(s):  
Kuo He ◽  
Xiuyuan Zhang ◽  
Ruiping Zhao ◽  
Lixia Wang ◽  
Tingting Feng ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Recombinant Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test

Ultrasensitive immunochromatographic strip assay for the detection of diminazene

The Analyst ◽  
2021 ◽  
Author(s):  
Mengjia chao ◽  
Xin-Xin Xu ◽  
Liqiang Liu ◽  
aihong wu ◽  
Shanshan Song ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Lateral Flow ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Flow Test ◽  
Lateral Flow Test

We prepared monoclonal antibodies (mAbs) against diminazene and used them in the development of a gold nanoparticle-based lateral-flow test (GNT) strip and indirect competitive enzyme-linked immunosorbent assay for the detection...

scholarly journals Development of a Sensitive Enzyme-Linked Immunosorbent Assay and Rapid Gold Nanoparticle Immunochromatographic Strip for Detecting Citrinin in Monascus Fermented Food

Toxins ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 354 ◽  
Author(s):  
Shih-Wei Wu ◽  
Yao-An Yu ◽  
Biing-Hui Liu ◽  
Feng-Yih Yu
Keyword(s):  
Detection Limit ◽  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Keyhole Limpet Hemocyanin ◽  
Fermented Food ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fermented Foods ◽  
Close Inspection ◽  
Immunochromatographic Strip ◽  
Rapid Antibody

Antibodies against citrinin (CTN) were generated from rabbits, which were injected with CTN-keyhole limpet hemocyanin (KLH). This work involved the development of a sensitive competitive direct enzyme-linked immunosorbent assay (cdELISA) and a rapid gold nanoparticle immunochromatographic strip (immunostrip) method for analyzing CTN in Monascus-fermented food. CTN at a concentration of 5.0 ng/mL caused 50% inhibition (IC50) of CTN-horseradish peroxidase (CTN-HRP) binding to the antibodies in the cdELISA. The capable on-site detection of CTN was accomplished by a rapid antibody-gold nanoparticle immunostrip with a detection limit of 20 ng/mL and that was completed within 15 min. A close inspection of 19 Monascus-fermented foods by cdELISA confirmed that 14 were contaminated with citrinin at levels from 28.6–9454 ng/g. Further analysis with the immunostrip is consistent with those results obtained using cdELISA. Both means are sensitive enough for the rapid examination of CTN in Monascus-fermented food products.

Gold Nanoparticle-Mediated High-Performance Enzyme-Linked Immunosorbent Assay for Detection of Tuberculosis ESAT-6 Protein

2017 ◽  
Vol 8 (2) ◽  
pp. 92-98 ◽  
Author(s):  
Thangavel Lakshmipriya ◽  
Subash C.B. Gopinath ◽  
Marimuthu Citartan ◽  
Uda Hashim ◽  
Thean-Hock Tang
Keyword(s):  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
High Performance ◽  
Enzyme Linked Immunosorbent Assay
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