An enzyme-linked immunosorbent assay and a gold-nanoparticle based immunochromatographic test for amatoxins using recombinant antibody

2016 ◽  
Vol 183 (7) ◽  
pp. 2211-2219 ◽  
Author(s):  
Kuo He ◽  
Xiuyuan Zhang ◽  
Ruiping Zhao ◽  
Lixia Wang ◽  
Tingting Feng ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Recombinant Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test

Development of Atom Transfer Radical Polymer-Modified Gold Nanoparticle-Based Enzyme-Linked Immunosorbent Assay (ELISA)

2014 ◽  
Vol 86 (20) ◽  
pp. 10021-10024 ◽  
Author(s):  
Feng Chen ◽  
Shike Hou ◽  
Qingsheng Li ◽  
Haojun Fan ◽  
Rong Fan ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Atom Transfer ◽  
Atom Transfer Radical Polymer

scholarly journals Poor Diagnostic Accuracy of Commercial Antibody-Based Assays for the Diagnosis of Acute Chikungunya Infection

2011 ◽  
Vol 18 (10) ◽  
pp. 1773-1775 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Ampai Tanganuchitcharnchai ◽  
Richard G. Jarman ◽  
Robert V. Gibbons ◽  
Daniel H. Paris ◽  
...  
Keyword(s):  
Virus Infection ◽  
Diagnostic Accuracy ◽  
Immunosorbent Assay ◽  
Chikungunya Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sri Lankan ◽  
Immunochromatographic Test

ABSTRACTA Sri Lankan fever cohort (n= 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.

scholarly journals Development of Rapid Immunochromatographic Test with Recombinant NcSAG1 for Detection of Antibodies to Neospora caninum in Cattle

2005 ◽  
Vol 12 (7) ◽  
pp. 885-887 ◽  
Author(s):  
Min Liao ◽  
Shoufa Zhang ◽  
Xuenan Xuan ◽  
Guohong Zhang ◽  
Xiaohong Huang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Reliable Method ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Serum Samples ◽  
Immunofluorescent Antibody Test ◽  
Good Agreement

ABSTRACT An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to clearly discriminate between immunofluorescent-antibody test (IFAT)-positive bovine sera and IFAT-negative bovine sera. Serum samples collected from cattle in Yanbian, China, were examined by the ICT. Of the 96 serum samples, 23 (24.0%) were positive by the ICT, and 19 (19.8%) samples were positive by a previously developed enzyme-linked immunosorbent assay (ELISA). Eighteen of 19 ELISA-positive samples were positive according to the ICT. A good agreement was found between the results of the ICT and the ELISA. The results presented here suggest that the ICT with recombinant truncated NcSAG1 fused to glutathione S-transferase is a useful and reliable method for the detection of antibodies to N. caninum in cattle.

scholarly journals Comparison between Immunochromatographic Test and Enzyme Linked Immunosorbent Assay in Diagnosis of Helicobacter pylori Infection among Gastritis Patients in Khartoum State-Sudan

2020 ◽  
pp. 48-53
Author(s):  
Omer Abu Elgasim ◽  
Salman Taha Ahmed Elmukashfi ◽  
Rayan Abdelwahid Mohammed ◽  
Adil Elamin Faroug
Keyword(s):  
Helicobacter Pylori ◽  
Immunosorbent Assay ◽  
Endoscopic Biopsy ◽  
Helicobacter Pylori Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rapid Urease Test ◽  
Immunochromatographic Test ◽  
Serological Tests ◽  
Urease Test ◽  
Immunoglobulin Gamma

Background: The gold standard for the diagnosis of Helicobacter pylori infection requires an endoscopic biopsy of gastric mucosa for histological examination, urease test and culture; however serological tests are useful as a screening test for Helicobacter pylori infection. Objective: To compare between Immunochromatographic Test and Enzyme Linked Immunosorbent Assay techniques in detection of Helicobacter pylori immunoglobulin gamma antibodies in serum of patients suffer from gastritis. Materials and Methods: 245 patients were screened for Helicobacter pylori infections by rapid urease test. Sera from these patients were tested for anti- Helicobacter pylori immunoglobulin gamma antibodies by Enzyme Linked Immunosorbent Assay and Immunochromatographic Test techniques. Results: Of 245 patients tested, Immunochromatographic Test positive/negative 114 (46.5%)/131 (53.5%), whereas Enzyme Linked Immunosorbent Assay positive/negative were 124 (50.6%)/121 (49.4%). Sensitivity/ specificity was 67.4%/74.5% and 90.2%/89.3% for Immunochromatographic Test/Enzyme Linked Immunosorbent Assay positive/negative, respectively. The diagnostic accuracy was 71%/89.7% for Immunochromatographic Test/Enzyme Linked Immunosorbent Assay, respectively. Conclusion: The Enzyme Linked Immunosorbent Assay technique was found to be more sensitive, specific and accurate compared to the Immunochromatographic Test while The Immunochromatographic Test is commercially available, inexpensive and easy to perform compared to the Enzyme Linked Immunosorbent Assay.

scholarly journals Comparison of a New Immunochromatographic Test to Enzyme-Linked Immunosorbent Assay for Rapid Detection of Immunoglobulin M Antibodies to Hepatitis E Virus in Human Sera

2005 ◽  
Vol 12 (5) ◽  
pp. 593-598 ◽  
Author(s):  
Hsiao Ying Chen ◽  
Yang Lu ◽  
Teresa Howard ◽  
David Anderson ◽  
Priscilla Yiquan Fong ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Immunosorbent Assay ◽  
Predictive Value ◽  
Rapid Detection ◽  
Hepatitis E Virus ◽  
Rapid Test ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Acute Hepatitis E

ABSTRACT An immunochromatographic test for rapid detection of IgM antibodies in patients with acute hepatitis E infection was developed utilizing the well-characterized recombinant protein EP2.1 and monoclonal antibody 4B2. The new rapid test based on a novel reverse-flow technology was able to generate a positive result within 2 to 3 min. Our study showed that this test was able to detect anti-HEV IgM antibodies in 96.7% of the patient samples tested (n = 151) while maintaining an excellent specificity of 98.6% with samples from various patient or healthy control groups (total n = 208). Furthermore, this rapid test gave a good specificity of 90.9% when tested with rheumatoid factor (RF)-positive sera (RF value of ≤850 IU/ml; n = 11) although a higher concentration of RF in samples might cause cross-reactivity. The new test has a good agreement of 97.2% with a kappa value of 0.943 when compared with a reference enzyme-linked immunosorbent assay. The positive predictive value and the negative predictive value for the rapid test thus reached 98.0 and 97.6%, respectively. This is the first rapid, point-of-care test for hepatitis E and will be especially useful for the diagnosis of acute hepatitis E virus infection in field and emergency settings and in resource-poor countries.

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