Aurone Derivative Revealing the Metabolism of Lipid Droplets and Monitoring Oxidative Stress in Living Cells

2020 ◽  
Vol 92 (9) ◽  
pp. 6631-6636 ◽  
Author(s):  
Kangnan Wang ◽  
Shuyue Ma ◽  
Yanyan Ma ◽  
Yuping Zhao ◽  
Miaomiao Xing ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Droplets ◽  
Living Cells

A Molecular Logic Gate Enables Super-Resolved Imaging of Intracellular Lipid Droplets

2019 ◽  
Author(s):  
Adam Eördögh ◽  
Carolina Paganini ◽  
Dorothea Pinotsi ◽  
Paolo Arosio ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Single Molecule ◽  
Lipid Droplets ◽  
Neutral Lipids ◽  
Logic Gate ◽  
Living Cells ◽  
Three Dimensions ◽  
Single Molecule Imaging ◽  
Molecular Logic Gate ◽  
Molecular Logic ◽  
Cellular Compartments

<div>Photoactivatable dyes enable single-molecule imaging in biology. Despite progress in the development of new fluorophores and labeling strategies, many cellular compartments remain difficult to image beyond the limit of diffraction in living cells. For example, lipid droplets, which are organelles that contain mostly neutral lipids, have eluded single-molecule imaging. To visualize these challenging subcellular targets, it is necessary to develop new fluorescent molecular devices beyond simple on/off switches. Here, we report a fluorogenic molecular logic gate that can be used to image single molecules associated with lipid droplets with excellent specificity. This probe requires the subsequent action of light, a lipophilic environment and a competent nucleophile to produce a fluorescent product. The combination of these requirements results in a probe that can be used to image the boundary of lipid droplets in three dimensions with resolutions beyond the limit of diffraction. Moreover, this probe enables single-molecule tracking of lipids within and between droplets in living cells.</div>

Highly sensitive real-time detection of intracellular oxidative stress and application in mycotoxin toxicity evaluation based on living single-cell electrochemical sensors

The Analyst ◽  
2021 ◽  
Author(s):  
Lu Gao ◽  
Jiadi Sun ◽  
Liping Wang ◽  
Qigao Fan ◽  
Gaowen Zhu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Electrochemical Sensor ◽  
Single Cell ◽  
Real Time ◽  
Electrochemical Sensors ◽  
Living Cells ◽  
Selective Detection ◽  
Toxicity Evaluation ◽  
Highly Sensitive ◽  
Intracellular Oxidative Stress

Single-cell electrochemical sensor is used in the local selective detection of living cells because of its high spatial–temporal resolution and sensitivity, as well as its ability to obtain comprehensive cellular physiological states and processes.

scholarly journals Effects of monoolein-based cubosome formulations on lipid droplets and mitochondria of HeLa cells

2015 ◽  
Vol 4 (4) ◽  
pp. 1025-1036 ◽  
Author(s):  
Angela Maria Falchi ◽  
Antonella Rosa ◽  
Angela Atzeri ◽  
Alessandra Incani ◽  
Sandrina Lampis ◽  
...  
Keyword(s):  
Hela Cells ◽  
Lipid Droplets ◽  
Living Cells

Analysis of living cells after staining with organelle-specific dyes shows that monoolein-based cubosome treatment induces accumulation of lipid droplets (green) and mitochondrial (red) hyperpolarization.

scholarly journals Visualizing Cancer Cell Metabolic Dynamics Regulated With Aromatic Amino Acids Using DO-SRS and 2PEF Microscopy

2021 ◽  
Vol 8 ◽  
Author(s):  
Pegah Bagheri ◽  
Khang Hoang ◽  
Anthony A. Fung ◽  
Sahran Hussain ◽  
Lingyan Shi
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Protein Synthesis ◽  
Spatial Distribution ◽  
Lipid Droplets ◽  
De Novo ◽  
Culture Media ◽  
Aromatic Amino Acids ◽  
Unsaturated Lipids ◽  
Metabolic Activities

Oxidative imbalance plays an essential role in the progression of many diseases that include cancer and neurodegenerative diseases. Aromatic amino acids (AAA) such as phenylalanine and tryptophan have the capability of escalating oxidative stress because of their involvement in the production of Reactive Oxygen Species (ROS). Here, we use D2O (heavy water) probed stimulated Raman scattering microscopy (DO-SRS) and two Photon Excitation Fluorescence (2PEF) microscopy as a multimodal imaging approach to visualize metabolic changes in HeLa cells under excess AAA such as phenylalanine or trytophan in culture media. The cellular spatial distribution of de novo lipogenesis, new protein synthesis, NADH, Flavin, unsaturated lipids, and saturated lipids were all imaged and quantified in this experiment. Our studies reveal ∼10% increase in de novo lipogenesis and the ratio of NADH to flavin, and ∼50% increase of the ratio of unsaturated lipids to saturated lipid in cells treated with excess phenylalanine or trytophan. In contrast, these cells exhibited a decrease in the protein synthesis rate by ∼10% under these AAA treatments. The cellular metabolic activities of these biomolecules are indicators of elevated oxidative stress and mitochondrial dysfunction. Furthermore, 3D reconstruction images of lipid droplets were acquired and quantified to observe their spatial distribution around cells’ nuceli under different AAA culture media. We observed a higher number of lipid droplets in excess AAA conditions. Our study showcases that DO-SRS imaging can be used to quantitatively study how excess AAA regulates metabolic activities of cells with subcellular resolution in situ.

scholarly journals Lipid droplets alleviate cadmium induced cytotoxicity in Saccharomyces cerevisiae

2017 ◽  
Vol 6 (1) ◽  
pp. 30-41 ◽  
Author(s):  
Selvaraj Rajakumar ◽  
Vasanthi Nachiappan
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Reactive Oxygen Species ◽  
Lipid Accumulation ◽  
Lipid Droplets ◽  
Reactive Oxygen ◽  
Oxygen Species

Cadmium (Cd) induces oxidative stress that generates reactive oxygen species (ROS) and increased lipid accumulation.

scholarly journals Increasing the Resistance of Living Cells against Oxidative Stress by Nonnatural Surfactants as Membrane Guards

2018 ◽  
Vol 10 (28) ◽  
pp. 23638-23646 ◽  
Author(s):  
Marius Kunkel ◽  
Stefan Schildknecht ◽  
Klaus Boldt ◽  
Lukas Zeyffert ◽  
David Schleheck ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Living Cells

New structural and functional insights from in-cell NMR

2018 ◽  
Vol 2 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Enrico Luchinat ◽  
Lucia Banci
Keyword(s):  
Oxidative Stress ◽  
Structural Biology ◽  
Structural Characterization ◽  
Living Cells ◽  
Atomic Resolution ◽  
Structural And Functional Properties ◽  
Cellular Context ◽  
Dynamical Properties ◽  
Partner Proteins ◽  
And Oxidative Stress

In recent years, it has become evident that structural characterization would gain significantly in terms of biological relevance if framed within a cellular context, while still maintaining the atomic resolution. Therefore, major efforts have been devoted to developing Cellular Structural Biology approaches. In this respect, in-cell NMR can provide and has provided relevant contributions to the field, not only to investigate the structural and dynamical properties of macromolecules in solution but, even more relevant, to understand functional processes directly in living cells and the factors that modulate them, such as exogenous molecules, partner proteins, and oxidative stress. In this commentary, we review and discuss some of the main contributions to the understanding of protein structural and functional properties achieved by in-cell NMR.

Oxidative stress and living cells

1995 ◽  
Vol 40 (2) ◽  
pp. 131-152 ◽  
Author(s):  
G. Gille ◽  
K. Sigler
Keyword(s):  
Oxidative Stress ◽  
Living Cells
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