A System-Wide Spatiotemporal Characterization of ErbB Receptor Complexes by Subcellular Fractionation Integrated Quantitative Mass Spectrometry

2021 ◽  
Author(s):  
Shujuan Wang ◽  
Cunjie Zhang ◽  
Mansheng Li ◽  
Chao Zhao ◽  
Yong Zheng
Keyword(s):  
Mass Spectrometry ◽  
Subcellular Fractionation ◽  
Erbb Receptor ◽  
Quantitative Mass Spectrometry ◽  
Receptor Complexes

scholarly journals Proteomic Analysis Demonstrates Activator- and Chromatin-specific Recruitment to Promoters

2012 ◽  
Vol 287 (42) ◽  
pp. 35397-35408 ◽  
Author(s):  
Timothy W. Sikorski ◽  
Yoo Jin Joo ◽  
Scott B. Ficarro ◽  
Manor Askenazi ◽  
Stephen Buratowski ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Saccharomyces Cerevisiae ◽  
Rna Polymerase Ii ◽  
Histone Acetyltransferases ◽  
Quantitative Mass Spectrometry ◽  
Dna Templates ◽  
Naked Dna ◽  
Transcription Activators ◽  
Basal Transcription Factors

In-depth characterization of RNA polymerase II preinitiation complexes remains an important and challenging goal. We used quantitative mass spectrometry to explore context-dependent Saccharomyces cerevisiae preinitiation complex formation at the HIS4 promoter reconstituted on naked and chromatinized DNA templates. The transcription activators Gal4-VP16 and Gal4-Gcn4 recruited a limited set of chromatin-related coactivator complexes, namely the chromatin remodeler Swi/Snf and histone acetyltransferases SAGA and NuA4, suggesting that transcription stimulation is mediated through these factors. Moreover, the two activators differentially recruited the coactivator complexes, consistent with specific activator-coactivator interactions. Chromatinized templates suppressed recruitment of basal transcription factors, thereby amplifying the effect of activators, compared with naked DNA templates. This system is sensitive, highly reproducible, and easily applicable to mapping the repertoire of proteins found at any promoter.

scholarly journals Characterization of the Small Intestinal Lesion in Celiac Disease by Label-Free Quantitative Mass Spectrometry

2018 ◽  
Vol 188 (7) ◽  
pp. 1563-1579 ◽  
Author(s):  
Astrid E.V. Tutturen ◽  
Siri Dørum ◽  
Trevor Clancy ◽  
Henrik M. Reims ◽  
Asbjørn Christophersen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Celiac Disease ◽  
Label Free ◽  
Quantitative Mass Spectrometry ◽  
Intestinal Lesion ◽  
Small Intestinal Lesion ◽  
Small Intestinal

scholarly journals Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane

2004 ◽  
Vol 167 (6) ◽  
pp. 1099-1112 ◽  
Author(s):  
Marcello Marelli ◽  
Jennifer J. Smith ◽  
Sunhee Jung ◽  
Eugene Yi ◽  
Alexey I. Nesvizhskii ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Large Scale ◽  
Secretory Pathway ◽  
Subcellular Fractionation ◽  
Membrane Dynamics ◽  
Small Gtpase ◽  
Quantitative Mass Spectrometry ◽  
Actin Organization ◽  
Associated Proteins ◽  
Peroxisome Membrane

We have combined classical subcellular fractionation with large-scale quantitative mass spectrometry to identify proteins that enrich specifically with peroxisomes of Saccharomyces cerevisiae. In two complementary experiments, isotope-coded affinity tags and tandem mass spectrometry were used to quantify the relative enrichment of proteins during the purification of peroxisomes. Mathematical modeling of the data from 306 quantified proteins led to a prioritized list of 70 candidates whose enrichment scores indicated a high likelihood of them being peroxisomal. Among these proteins, eight novel peroxisome-associated proteins were identified. The top novel peroxisomal candidate was the small GTPase Rho1p. Although Rho1p has been shown to be tethered to membranes of the secretory pathway, we show that it is specifically recruited to peroxisomes upon their induction in a process dependent on its interaction with the peroxisome membrane protein Pex25p. Rho1p regulates the assembly state of actin on the peroxisome membrane, thereby controlling peroxisome membrane dynamics and biogenesis.

scholarly journals One-Pot Shotgun Quantitative Mass Spectrometry Characterization of Histones

2009 ◽  
Vol 8 (11) ◽  
pp. 5367-5374 ◽  
Author(s):  
Mariana D. Plazas-Mayorca ◽  
Barry M. Zee ◽  
Nicolas L. Young ◽  
Ian M. Fingerman ◽  
Gary LeRoy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Mass Spectrometry ◽  
One Pot

scholarly journals Innovative Application of Mass Spectrometry for the Characterization of Staphylococcal Enterotoxins Involved in Food Poisoning Outbreaks

2008 ◽  
Vol 75 (3) ◽  
pp. 882-884 ◽  
Author(s):  
Jacques-Antoine Hennekinne ◽  
Virginie Brun ◽  
Marie-Laure De Buyser ◽  
Alain Dupuis ◽  
Annick Ostyn ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Food Poisoning ◽  
Quantitative Mass Spectrometry ◽  
Staphylococcal Enterotoxins ◽  
Common Food ◽  
Food Borne ◽  
First Time

ABSTRACT Staphylococcal poisoning is a common food-borne disease for which immunoassays to detect enterotoxins were developed, but these assays often lead to false diagnoses due to interferences or lack of specificity. Absolute quantitative mass spectrometry was for the first time successfully applied to an investigation of a staphylococcal outbreak due to coconut pearls.

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