Real-Time Visualization of Embryonic Apoptosis Using a Near-Infrared Fluorogenic Probe for Embryo Development Evaluation

2021 ◽  
Author(s):  
Lei He ◽  
Shuyue Ye ◽  
Jing Fang ◽  
Yuqi Zhang ◽  
Chaoxiang Cui ◽  
...  
Keyword(s):  
Real Time ◽  
Embryo Development ◽  
Near Infrared ◽  
Fluorogenic Probe ◽  
Real Time Visualization ◽  
Development Evaluation

Real-Time Visualization and Monitoring of Physiological Dynamics by Aggregation-Induced Emission Luminogens (AIEgens)

2021 ◽  
Vol 14 (1) ◽  
pp. 413-435
Author(s):  
Xuewen He ◽  
Jacky W.Y. Lam ◽  
Ryan T.K. Kwok ◽  
Ben Zhong Tang
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Molecular Design ◽  
High Sensitivity ◽  
Microwave Emission ◽  
Excellent Performance ◽  
Disease Evolution ◽  
Aggregation Induced Emission ◽  
Long Wavelength ◽  
Real Time Visualization

Physiological dynamics in living cells and tissues are crucial for maintenance and regulation of their normal activities and functionalities. Tiny fluctuations in physiological microenvironments can leverage significant influences on cell growth, metabolism, differentiation, and apoptosis as well as disease evolution. Fluorescence imaging based on aggregation-induced emission luminogens (AIEgens) exhibits superior advantages in real-time sensing and monitoring of the physiological dynamics in living systems, including its unique properties such as high sensitivity and rapid response, flexible molecular design, and versatile nano- to mesostructural fabrication. The introduction of canonic AIEgens with long-wavelength, near-infrared, or microwave emission, persistent luminescence, and diversified excitation source (e.g., chemo- or bioluminescence) offers researchers a tool to evaluate the resulting molecules with excellent performance in response to subtle fluctuations in bioactivities with broader dimensionalities and deeper hierarchies.

scholarly journals Near-infrared fluorescent cholangiography – real-time visualization of the biliary tree during elective laparoscopic cholecystectomy

HPB ◽  
2018 ◽  
Vol 20 (6) ◽  
pp. 538-545 ◽  
Author(s):  
Antonio Pesce ◽  
Saverio Latteri ◽  
Martina Barchitta ◽  
Teresa R. Portale ◽  
Biagio Di Stefano ◽  
...  
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Real Time ◽  
Near Infrared ◽  
Biliary Tree ◽  
Fluorescent Cholangiography ◽  
Real Time Visualization ◽  
Elective Laparoscopic Cholecystectomy

scholarly journals Real‐time Visualization and Quantification of Retrograde Cardioplegia Delivery using Near Infrared Fluorescent Imaging

2008 ◽  
Vol 23 (6) ◽  
pp. 701-708 ◽  
Author(s):  
Aravind T. Rangaraj ◽  
Ravi K. Ghanta ◽  
Ramanan Umakanthan ◽  
Edward G. Soltesz ◽  
Rita G. Laurence ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Retrograde Cardioplegia ◽  
Real Time Visualization

A Ratiometric Near-Infrared Fluorogen for the Real Time Visualization of Intracellular Redox Status during Apoptosis

2017 ◽  
Vol 23 (30) ◽  
pp. 7191-7195 ◽  
Author(s):  
Giridharan Saranya ◽  
Palapuravan Anees ◽  
Manu M. Joseph ◽  
Kaustabh K. Maiti ◽  
Ayyappanpillai Ajayaghosh
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Redox Status ◽  
The Real ◽  
Real Time Visualization ◽  
Intracellular Redox Status ◽  
Intracellular Redox

Real-Time Visualization of Ureters Using Indocyanine Green During Laparoscopic Surgeries: Can We Make Surgery Safer?

2019 ◽  
Vol 26 (4) ◽  
pp. 464-468 ◽  
Author(s):  
Pranav Mandovra ◽  
Vishaka Kalikar ◽  
Roy V. Patankar
Keyword(s):  
Indocyanine Green ◽  
Real Time ◽  
Near Infrared ◽  
Ureteral Injury ◽  
Specific Method ◽  
Camera System ◽  
Ureteral Catheter ◽  
Light Mode ◽  
Real Time Visualization ◽  
Intraoperative Localization

Background. Intraoperative ureteral injury is rare, but a grave complication during laparoscopic surgery. Several methods for intraoperative localization of ureters are described with their own pitfalls. Intraoperative localization using near-infrared (NIR) fluorescence with indocyanine green (ICG) is an easier and assured method during laparoscopic pelvic surgeries. Method. From September 2017 to December 2017, patients undergoing laparoscopic pelvic surgeries were administered cystoscopic-guided intraureteral ICG immediately preoperatively with tip of a 6-Fr ureteral catheter. The fluorescence of ureters was visualized in the NIR mode of the camera system, localizing the ureters precisely and in real time. Results. This technique was used to visualize ureters in 30 surgeries. Median age of the patients was 46.7 years with median body mass index of 23.2 kg/m2. Mean duration between administration of dye and insertion of trocar was 10 minutes. Mean duration for insertion of cystoscopically guided intraureteral ICG was 7 minutes. Ureteral fluorescence was visualized in all cases with some variation in intensity of the brightness perceived depending on surrounding fat. Duration of the lengthiest surgery was 240 minutes, and fluorescence was appreciated till the end. There were no intraoperative or postoperative complications attributed to ICG administration. In 10 patients (33%), there was difficulty in identifying the ureters on conventional white light mode, in which ICG localization was extremely helpful. Conclusion. ICG-stained ureteral visualization under NIR light is a safe and feasible method that provides real-time ureteral demarcation. This easily replicable, sensitive, and specific method of ureteral visualization can make complex laparoscopic pelvic surgeries safer.

Azo-based near-infrared fluorescent theranostic probe for tracking hypoxia-activated cancer chemotherapy in vivo

2019 ◽  
Vol 55 (87) ◽  
pp. 13172-13175 ◽  
Author(s):  
Ning Ding ◽  
Zhao Li ◽  
Xinwei Tian ◽  
Jiahang Zhang ◽  
Kaili Guo ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Cancer Chemotherapy ◽  
Near Infrared ◽  
Real Time Visualization

A novel azo-based near-infrared fluorescent therabostic probe activated by hypoxia is applied to real-time visualization of drug delivery in vivo.

Infrared fluorescence lymphography in experimental and clinical practice

2018 ◽  
Vol 17 (2) ◽  
pp. 84-91 ◽  
Author(s):  
G. V. Papayan ◽  
A. L. Akopov ◽  
P. A. Antonyan ◽  
A. A. Ilin ◽  
N. N. Petrishchev
Keyword(s):  
Lymph Nodes ◽  
Near Infrared ◽  
Lymphatic System ◽  
Lymphatic Vessels ◽  
Diagnostic System ◽  
Biological Tissues ◽  
Peritumoral Injection ◽  
Intradermal Administration ◽  
Nir Fluorescence ◽  
Real Time Visualization

Introduction. Near infrared (NIR) fluorescent diagnostics is promising due to a deeper penetration into biological tissues. Material and methods. In experiments on rabbits and in clinical studies evaluation the lymphatic system with the use of the instrument complex FLUM-808 was analysed. Results. For visualization of the lymphatic vessels of the skin, the intradermal administration of ICG, dissolved in 20 % albumin in the order of 0.02 mg/ml, is optimal. Peritumoral injection of ICG allows visualizing sentinel lymph nodes in patients with lung cancer. Conclusions. The developed NIR fluorescence diagnostic system FLUM-808 allows to real time visualization of lymphatic vessels and lymph nodes.

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