Development and Interlaboratory Validation of a Simple Screening Method for Genetically Modified Maize Using a ΔΔCq-Based Multiplex Real-Time PCR Assay

2016 ◽  
Vol 88 (8) ◽  
pp. 4285-4293 ◽  
Author(s):  
Akio Noguchi ◽  
Kosuke Nakamura ◽  
Kozue Sakata ◽  
Nozomi Sato-Fukuda ◽  
Takumi Ishigaki ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Screening Method ◽  
Genetically Modified Maize ◽  
Pcr Assay ◽  
Interlaboratory Validation ◽  
Simple Screening

scholarly journals Interlaboratory validation of an improved method for detection of Cyclospora cayetanensis in produce using a real-time PCR assay

2018 ◽  
Vol 69 ◽  
pp. 170-178 ◽  
Author(s):  
Helen R. Murphy ◽  
Hediye Nese Cinar ◽  
Gopal Gopinath ◽  
Kathy E. Noe ◽  
Lacresha D. Chatman ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Improved Method ◽  
Pcr Assay ◽  
Cyclospora Cayetanensis ◽  
Interlaboratory Validation

scholarly journals Effect of Feeding Cows Genetically Modified Maize on the Bacterial Community in the Bovine Rumen

2007 ◽  
Vol 73 (24) ◽  
pp. 8012-8017 ◽  
Author(s):  
S. Wiedemann ◽  
P. Gürtler ◽  
C. Albrecht
Keyword(s):  
Bacterial Community ◽  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Bacterial Strains ◽  
Maize Silage ◽  
Bovine Rumen ◽  
Gm Maize ◽  
Effect Of Feeding

ABSTRACT Rumen-cannulated cows (n = 4) were fed successively silage made from either conventional or genetically modified (GM) maize. Results revealed no effects of GM maize on the dynamics of six ruminal bacterial strains (investigated by real-time PCR) compared to the conventional maize silage.

Statistical Evaluation of Real-Time PCR Protocols Applied to Quantify Genetically Modified Maize

2010 ◽  
Vol 3 (4) ◽  
pp. 304-312 ◽  
Author(s):  
Silvia Folloni ◽  
Gianni Bellocchi ◽  
Adelina Prospero ◽  
Maddalena Querci ◽  
William Moens ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Statistical Evaluation ◽  
Genetically Modified ◽  
Genetically Modified Maize

scholarly journals Evaluation of a Real Time PCR Assay Method for the Detection of Genetically Modified Organisms in Food Products

2020 ◽  
Vol 9 (2) ◽  
pp. 1
Author(s):  
Eleni Spanea ◽  
Theofania Tsironi ◽  
Efstathia Tsakali ◽  
Anthimia Batrinou ◽  
Valentina Stefanou ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Food Products ◽  
Assay Method ◽  
Genetically Modified Maize ◽  
Nos Terminator ◽  
Repeatability And Reproducibility ◽  
Pcr Method

The objective of the study was to determine qualitatively by validated Real Time PCR method the occurrence of genetically modified maize and soybean in commercial food products from the Greek market. 70 independent samples were collected, including products from different categories (i.e. cereal based, biscuits and snacks) which declared either corn or soybean on the labelling. The result of the study indicated that 37.1% of maize and soy products (n=70) displayed in the Greek market have detectable levels of genetically modified maize or soy. These products were identified by specific primers and included common GMΟ detection primers for 35S and NOS terminator. Adequate repeatability and reproducibility was demonstrated for the applied Real Time PCR method, as evaluated by intra- and inter-laboratory tests.

Molecular Identification of Four Genetically Modified Maize (Bt11, Bt176, Mon810 and T25) by Duplex Quantitative Real-Time PCR

2013 ◽  
Vol 7 (1) ◽  
pp. 224-233 ◽  
Author(s):  
Maher Chaouachi ◽  
Mohamed Salem Zellama ◽  
Nesrine Nabi ◽  
Ahmed Ben Hafsa ◽  
Khaled Saïd
Keyword(s):  
Real Time ◽  
Molecular Identification ◽  
Real Time Pcr ◽  
Genetically Modified ◽  
Genetically Modified Maize ◽  
Quantitative Real Time Pcr
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