Fabrication of Magnetic Conjugation Clusters via Intermolecular Assembling for Ultrasensitive Surface Plasmon Resonance (SPR) Detection in a Wide Range of Concentrations

2017 ◽  
Vol 89 (24) ◽  
pp. 13472-13479 ◽  
Author(s):  
Zhichao Lou ◽  
He Han ◽  
Ming Zhou ◽  
Jinfeng Wan ◽  
Qian Sun ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Wide Range

scholarly journals Determination of Vitamin B12 in Fortified Bovine Milk- Based Infant Formula Powder, Fortified Soya-Based Infant Formula Powder, Vitamin Premix, and Dietary Supplements by Surface Plasmon Resonance: Collaborative Study

2011 ◽  
Vol 94 (4) ◽  
pp. 1217-1226 ◽  
Author(s):  
Pathik Vyas ◽  
Anthony A O'kane ◽  
E Ager ◽  
S Crooks ◽  
C Elliott ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Protein Binding ◽  
Vitamin B12 ◽  
Infant Formula ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Protein ◽  
Collaborative Study ◽  
Wide Range

Abstract A collaborative study was conducted on an inhibition-based protein-binding assay using the Biacore Q™ biosensor instrument and the Biacore Qflex™ Kit Vitamin B12 PI. The samples studied included infant formula, cereals, premixes, vitamin tablets, dietary supplements, and baby food. The collaborative study, which involved 11 laboratories, demonstrated that the assay showed an RSDr of 1.59–27.8 and HorRat values for reproducibility of 0.34–1.89 in samples with levels ranging from ppm to ppb. The assay studied is a label-free protein binding-based assay that uses the principle of surface plasmon resonance (SPR) to measure the interaction between vitamin B12 and a specifc binding protein. A Biacore Q biosensor uses this principle to detect binding directly at the surface of a sensor chip with a hydrophilic gold-dextran surface. The instrument passes a mixture of prepared sample extract and binding protein solution across a covalently immobilized vitamin B12 chip surface, and the response is given as free-binding protein as the mixture binds to the immobilized surface. This technique uses the specifcity and robustness of the protein-ligand interaction to allow minimal sample preparation and a wide range of matrixes to be analyzed rapidly. The reagents and accessories needed to perform this assay are provided as the ready-to-use format “Qflex Kit Vitamin B12 PI.” The method is intended for routine use in the quantitative determination of vitamin B12 (as cyanocobalamin) in a wide range of food products, dietary vitamin supplements, and multivitamin premixes.

Rapid surface plasmon resonance immunoassays for the determination of mycotoxins in cereals and cereal-based food products

2014 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
J.P. Meneely ◽  
C.T. Elliott
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cost Effective ◽  
Food Products ◽  
Future Research ◽  
Portable Devices ◽  
Wide Range ◽  
Food And Feed ◽  
Robust Sensing

In recent times surface plasmon resonance has demonstrated its applicability to the detection of a wide range of contaminants in food and feed including mycotoxins in cereals and cereal-based food products. Commercially available, laboratory-based systems have exploited high affinity polyclonal, monoclonal and recombinant antibodies and robust sensing surfaces to provide rapid, accurate and sensitive means of determining these toxins. In addition many custom-built, prototype devices have shown a great deal of potential for this particular application and have included the combination of surface plasmon resonance with enzyme-derivatised sensors, molecularly imprinted polymers, fluorescence spectroscopy and the use of gold nanoparticles for signal enhancement. Of note is the lack of available devices that allow the detection of multiple mycotoxins simultaneously and portable devices that could be used in the field, therefore future research and development should focus on these areas to deliver cost-effective miniaturised devices with multiplexing capabilities.

scholarly journals Topological insulator overlayer to enhance the sensitivity and detection limit of surface plasmon resonance sensor

Nanophotonics ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1941-1951
Author(s):  
Jiaqi Zhu ◽  
Yuxuan Ke ◽  
Jianfeng Dai ◽  
Qi You ◽  
Leiming Wu ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Label Free ◽  
Spr Sensor ◽  
Wide Range ◽  
Resonance Sensor ◽  
Label Free Detection ◽  
Potential Applications

AbstractSurface plasmon resonance (SPR) sensors have been applied in a wide range of applications for real-time and label-free detection. In this article, by covering the topological insulators nanosheets on the surface of the noble metal (Au), the sensitivity of the SPR sensor is greatly enhanced because of the strong interaction of light with Au–bismuth selenide (Bi2Se3) heterostructure. It is shown that the sensitivity of proposed SPR sensors depends on the concentration of Bi2Se3 solution or the thickness of the coated Bi2Se3 film. The optimised sensitivity (2929.1 nm/RIU) and figure of merit (33.45 RIU−1) have been obtained after three times drop-casting, and the enhancement sensitivity of proposed sensors is up to 51.97% compared to the traditional Au–SPR sensors. Meanwhile, the reflection spectrum is simulated by using the method of effective refractive index, and the reason for the increase of sensitivity is analysed theoretically. For researching the application of modified SPR sensor, heavy metal detection is employed to detect in the last part. Our proposed SPR sensors have potential applications in heavy metal detections and biosensing.

scholarly journals A RAGE-Targeted Antibody-Drug Conjugate: Surface Plasmon Resonance as a Platform for Accelerating Effective ADC Design and Development

Antibodies ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Gareth D. Healey ◽  
Asa Frostell ◽  
Tim Fagge ◽  
Deyarina Gonzalez ◽  
R. Steven Conlan
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Antibody Drug Conjugate ◽  
Antibody Drug Conjugates ◽  
Drug Conjugate ◽  
Drug Conjugates ◽  
Wide Range ◽  
Endosomal Ph ◽  
Antibody Drug

Antibodies, antibody-like molecules, and therapeutics incorporating antibodies as a targeting moiety, such as antibody-drug conjugates, offer significant potential for the development of highly efficacious drugs against a wide range of disorders. Despite some success, truly harnessing the superior targeting properties of these molecules requires a platform from which to effectively identify the best candidates for drug development. To streamline the development of antibody-drug conjugates targeting gynecological cancers within our laboratory, we incorporated surface plasmon resonance analysis (Biacore™ T200) into our development toolkit. Antibodies, selected based on positive ELISA screens as suitable for development as antibody-drug conjugates, were evaluated using surface plasmon resonance to determine a wide range of characteristics including specificity, kinetics/affinity, the effect of linker binding, the impact of the drug to antibody ratio, and the effect of endosomal pH on antibody-antigen binding. Analysis revealed important kinetics data and information regarding the effect of conjugation and endosomal pH on our antibody candidates that correlated with cell toxicity and antibody internalization data. As well as explaining observations from cell-based assays regarding antibody-drug conjugate efficacies, these data also provide important information regarding intelligent antibody selection and antibody-drug conjugate design. This study demonstrates the application of surface plasmon resonance technology as a platform, where detailed information can be obtained, supporting the requirements for rapid and high-throughput screening that will enable enhanced antibody-drug conjugate development.

scholarly journals Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 906
Author(s):  
Alessandra Camarca ◽  
Antonio Varriale ◽  
Alessandro Capo ◽  
Angela Pennacchio ◽  
Alessia Calabrese ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Fluorescence Spectroscopy ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Food Environment ◽  
Daily Life ◽  
Commercial Potential ◽  
Scientific Disciplines ◽  
Wide Range

The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

Control of localized surface plasmon resonance energy in monolayer structures of gold and silver nanoparticles

2015 ◽  
Vol 17 (40) ◽  
pp. 27077-27081 ◽  
Author(s):  
Hiroki Yokota ◽  
Taichi Taniguchi ◽  
Taichi Watanabe ◽  
DaeGwi Kim
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Ag Nanoparticles ◽  
Localized Surface Plasmon Resonance ◽  
Resonance Energy ◽  
Localized Surface Plasmon ◽  
Gold And Silver Nanoparticles ◽  
Wide Range

The LSPR energy was successfully controlled in the wide range of 2.0–3.0 eV using Au and Ag nanoparticles.

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