Target-Induced Horseradish Peroxidase Deactivation for Multicolor Colorimetric Assay of Hydrogen Sulfide in Rat Brain Microdialysis

2018 ◽  
Vol 90 (10) ◽  
pp. 6222-6228 ◽  
Author(s):  
Zhonghui Chen ◽  
Chaoqun Chen ◽  
Huawei Huang ◽  
Fang Luo ◽  
Longhua Guo ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Horseradish Peroxidase ◽  
Rat Brain ◽  
Colorimetric Assay ◽  
Brain Microdialysis

Chemiluminescent sensor for hydrogen sulfide in rat brain microdialysis based on target-induced horseradish peroxidase deactivation

2019 ◽  
Vol 11 (24) ◽  
pp. 3085-3089 ◽  
Author(s):  
Zheng Fang ◽  
Guiyin Yue ◽  
Jian Wang ◽  
Fang Luo ◽  
Longhua Guo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Sulfide ◽  
Horseradish Peroxidase ◽  
Rat Brain ◽  
Brain Microdialysis ◽  
Neuronal Transmission ◽  
The Brain

The detection of hydrogen sulfide (H2S) is important due to its role in the diagnosis of many diseases since H2S is involved in the protection of neurons from oxidative stress and neuronal transmission modulation in the brain.

Sensitive Fluorescent Sensor for Hydrogen Sulfide in Rat Brain Microdialysis via CsPbBr3 Quantum Dots

2019 ◽  
Vol 91 (24) ◽  
pp. 15915-15921 ◽  
Author(s):  
Chaoqun Chen ◽  
Qing Cai ◽  
Fang Luo ◽  
Nuo Dong ◽  
Longhua Guo ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Hydrogen Sulfide ◽  
Rat Brain ◽  
Fluorescent Sensor ◽  
Brain Microdialysis ◽  
Cspbbr3 Quantum Dots

Determination of fluoxetine and norfluoxetine in rat brain microdialysis samples following intraperitoneal fluoxetine administration

2004 ◽  
Vol 522 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Takeshi Fukushima ◽  
Emi Naka-aki ◽  
Xingjie Guo ◽  
Famei Li ◽  
Tineke Vankeirsbilck ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Microdialysis

scholarly journals In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device

Chemosensors ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Jae-Hyung Kim ◽  
Young-Ju Lee ◽  
Yong-Jin Ahn ◽  
Minyoung Kim ◽  
Gi-Ja Lee
Keyword(s):  
Prostate Cancer ◽  
Cell Culture ◽  
Hydrogen Sulfide ◽  
Colorimetric Assay ◽  
Lncap Cells ◽  
Prostate Cancer Cells ◽  
In Situ Detection ◽  
H2s Production ◽  
Analytical Device

In this study, a paper-integrated analytical device that combined a paper-based colorimetric assay with a paper-based cell culture platform was developed for the in situ detection of hydrogen sulfide (H2S) in three-dimensional (3D)-cultured, live prostate cancer cells. Two kinds of paper substrates were fabricated using a simple wax-printing methodology to form the cell culture and detection zones, respectively. LNCaP cells were seeded directly on the paper substrate and grown in the paper-integrated analytical device. The cell viability and H2S production of LNCaP cells were assessed using a simple water-soluble tetrazolium salt colorimetric assay and H2S-sensing paper, respectively. The H2S-sensing paper showed good sensitivity (sensitivity: 6.12 blue channel intensity/μM H2S, R2 = 0.994) and a limit of quantification of 1.08 μM. As a result, we successfully measured changes in endogenous H2S production in 3D-cultured, live LNCaP cells within the paper-integrated analytical device while varying the duration of incubation and substrate concentration (L-cysteine). This paper-integrated analytical device can provide a simple and effective method to investigate H2S signaling pathways and drug screening in a 3D culture model.

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