Strong and Biostable Hyaluronic Acid–Calcium Phosphate Nanocomposite Hydrogel via in Situ Precipitation Process

AbstractBiocementation is commonly based on microbial-induced carbonate precipitation (MICP) or enzyme-induced carbonate precipitation (EICP), where biomineralization of $$\text {CaCO}_{3}$$ CaCO 3 in a granular medium is used to produce a sustainable, consolidated porous material. The successful implementation of biocementation in large-scale applications requires detailed knowledge about the micro-scale processes of $$\text {CaCO}_{3}$$ CaCO 3 precipitation and grain consolidation. For this purpose, we present a microscopy sample cell that enables real time and in situ observations of the precipitation of $$\text {CaCO}_{3}$$ CaCO 3 in the presence of sand grains and calcite seeds. In this study, the sample cell is used in combination with confocal laser scanning microscopy (CLSM) which allows the monitoring in situ of local pH during the reaction. The sample cell can be disassembled at the end of the experiment, so that the precipitated crystals can be characterized with Raman microspectroscopy and scanning electron microscopy (SEM) without disturbing the sample. The combination of the real time and in situ monitoring of the precipitation process with the possibility to characterize the precipitated crystals without further sample processing, offers a powerful tool for knowledge-based improvements of biocementation.

Download Full-text

Development of an Injectable Calcium Phosphate/Hyaluronic Acid Microparticles System for Platelet Lysate Sustained Delivery Aiming Bone Regeneration

Macromolecular Bioscience ◽

10.1002/mabi.201600141 ◽

2016 ◽

Vol 16 (11) ◽

pp. 1662-1677 ◽

Cited By ~ 15

Author(s):

Pedro S. Babo ◽

Vítor E. Santo ◽

Manuela E. Gomes ◽

Rui L. Reis

Keyword(s):

Hyaluronic Acid ◽

Calcium Phosphate ◽

Bone Regeneration ◽

Platelet Lysate ◽

Sustained Delivery

Download Full-text

Degradation of hyaluronic acid does not prevent looping of the mammalian heart in situ

Developmental Biology ◽

10.1016/0012-1606(89)90281-9 ◽

1989 ◽

Vol 136 (2) ◽

pp. 555-559 ◽

Cited By ~ 35

Author(s):

H.Scott Baldwin ◽

Michael Solursh

Keyword(s):

Hyaluronic Acid ◽

Mammalian Heart

Download Full-text

The effect of adding hyaluronic acid to calcium phosphate on periapical tissue healing following periradicular surgery in dogs

Tanta Dental Journal ◽

10.1016/j.tdj.2014.07.001 ◽

2014 ◽

Vol 11 (2) ◽

pp. 122-129 ◽

Cited By ~ 4

Author(s):

Wafaa Ahmed Omar Segari ◽

Dalia Abd El Khalek Radwan ◽

Mohamed Ayad Abd El Hamid

Keyword(s):

Hyaluronic Acid ◽

Calcium Phosphate ◽

Tissue Healing

Download Full-text

Primary Study of an Injectable In Situ Composite of Collagen/Calcium Phosphate Porous Scaffold for Bone Substitute

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.857 ◽

2006 ◽

Vol 309-311 ◽

pp. 857-860 ◽

Cited By ~ 1

Author(s):

Q. Yao ◽

Dong Xiao Li ◽

K.W. Liu ◽

Bo Zhang ◽

H. Li ◽

...

Keyword(s):

Compressive Strength ◽

Calcium Phosphate ◽

Ph Value ◽

Porous Scaffold ◽

Setting Time ◽

Primary Study ◽

In Situ Composite ◽

Setting Process ◽

Main Material

This study was to develop an injectable biocompatible and porous calcium phosphate collagen composite cement scaffold by in situ setting. TTCP was prepared as main material of the CPC powder, and the collagen solution was added into the phosphoric acid directly to form the liquid phase. The injectable time (tI), setting time (tS) and setting temperature (TS), along with the PH value were recorded during the setting process. The compressive strength, morphology and porosity were tested. With the increase of collagen, this novel CPC get a tI of 5mins to 8mins, tS of 20mins to 30mins, compressive strength from 1.5MPa to 4MPa, and the porosity from 40% to 60%. This study gave a possibility to form a porous scaffold of collagen/CPC composite with the nature of injectability and setting in situ.

Download Full-text